Code | CSB-RA018327A05phHU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IHC | 1:50-1:200 |
IF | 1:20-1:200 |
To produce the phospho-POLR2A (S5) recombinant monoclonal antibody, the initial step entails isolating the genes encoding the POLR2A antibody from rabbits that have been previously immunized with a synthesized peptide derived from the human POLR2A protein phosphorylated at S5. Subsequently, these antibody genes are cloned into specialized expression vectors. Following this genetic modification, the vectors are introduced into host suspension cells. Following successful transfection, positive cells are cultured to promote the expression and secretion of antibodies. The phospho-POLR2A (S5) recombinant monoclonal antibody is then meticulously purified from the cell culture supernatant using affinity chromatography. Finally, the antibody's functionality is rigorously tested through a battery of assays, including ELISA, WB, IHC, and IF, confirming its ability to effectively react with human POLR2A protein phosphorylated at S5.
Phosphorylation of POLR2A at S5 is a fundamental regulatory step in eukaryotic gene expression. It helps orchestrate the complex transcriptional processes that lead to the synthesis of functional mRNA molecules, ensuring proper gene expression and cellular function. Dysregulation of this phosphorylation event can have significant implications for gene expression and is associated with various diseases and developmental disorders.
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