Alternative Names
PP2A A antibody; PP2A alpha antibody; PP2A B antibody; PP2A beta antibody; PP2A-alpha antibody; PP2A-beta antibody; PP2AA_HUMAN antibody; PP2Aalpha antibody; PP2AB_HUMAN antibody; PP2Abeta antibody; PP2Ac antibody; PP2CA antibody; PP2Calpha antibody; PP2CB antibody; PPP2CA antibody; PPP2CB antibody; Protein phosphatase 2 catalytic subunit alpha isoform antibody; Protein phosphatase 2 catalytic subunit beta isoform antibody; Protein phosphatase type 2A catalytic subunit antibody; Replication protein C antibody; RP C antibody; RP-C antibody; RPC antibody; Serine/threonine protein phosphatase 2A catalytic subunit alpha isoform antibody; Serine/threonine protein phosphatase 2A catalytic subunit beta isoform antibody; Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform antibody
Immunogen
A synthesized peptide derived from Human Phospho-PPP2CA (Y307)
Immunogen Species
Homo sapiens (Human)
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Tested Applications
ELISA, WB
Recommended Dilution
Application |
Recommended Dilution |
WB |
1:500-1:5000 |
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description
The production of the phospho-PPP2CA (Y307) recombinant monoclonal antibody involves the utilization of protein technology and DNA recombinant techniques. Initially, animals are immunized with a synthesized peptide derived from human phospho-PPP2CA (Y307), resulting in the generation of B cells. These B cells are then screened to isolate positive ones, followed by single clone identification. The light and heavy chains of the phospho-PPP2CA (Y307) antibody are amplified via PCR and integrated into a plasmid vector to construct a recombinant vector. This recombinant vector is subsequently transfected into host cells to facilitate antibody expression. The phospho-PPP2CA (Y307) recombinant monoclonal antibody is purified from the supernatant of cell culture using affinity chromatography. Stringent validation is conducted to ensure its accuracy and efficacy for ELISA and WB applications. The phospho-PPP2CA (Y307) recombinant monoclonal antibody serves as a valuable tool for detection of human phospho-PPP2CA (Y307) protein in research settings.