Code | CSB-RA805325A491phHU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
In the process of creating the phospho-PRKAA2 (S491) recombinant monoclonal antibody, the initial step involves the isolation of genes responsible for encoding the PRKAA2 antibody from rabbits that have been immunized with a synthesized peptide derived from the human PRKAA2 protein phosphorylated at S491. Subsequently, these antibody genes are cloned into expression vectors. Following this genetic modification, the modified vectors are carefully transfected into host suspension cells. Once transfection is successful, positive cells are cultivated to facilitate the expression and secretion of antibodies. The phospho-PRKAA2 (S491) recombinant monoclonal antibody is then purified from the cell culture supernatant using affinity chromatography. Finally, the antibody's activity is rigorously assessed through ELISA and WB tests, confirming its capability to interact effectively with the human PRKAA2 protein phosphorylated at S491.
Phosphorylation of PRKAA2 at S491 is a crucial regulatory event in the AMPK signaling pathway, allowing cells to adapt to energy fluctuations and maintain energy homeostasis. Dysregulation of this phosphorylation event can have significant implications for cellular metabolism and is implicated in various metabolic disorders and cancers.
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