Phospho-PRKAA2 (S491) Antibody

Code CSB-RA805325A491phHU
Size US$350Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • Western Blot
    Positive WB detected in 293 whole cell lysate,A549 whole cell lysate
    All lanes Phospho-PRKAA2 antibody at 1.1μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 62 KDa
    Observed band size: 62 KDa

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Product Details

Uniprot No. P54646
Target Names PRKAA2
Alternative Names 5'-AMP-activated protein kinase catalytic subunit alpha-2 antibody; AAPK2_HUMAN antibody; ACACA kinase antibody; Acetyl CoA carboxylase kinase antibody; Acetyl-CoA carboxylase kinase antibody; AMPK alpha 2 chain antibody; AMPK subunit alpha-2 antibody; AMPK2 antibody; AMPKa2 antibody; AMPKalpha2 antibody; HMGCR kinase antibody; Hydroxymethylglutaryl CoA reductase kinase antibody; Hydroxymethylglutaryl-CoA reductase kinase antibody; PRKAA antibody; PRKAA2 antibody; Protein kinase AMP activated alpha 2 catalytic subunit antibody; Protein kinase AMP activated catalytic subunit alpha 2 antibody
Species Reactivity Human
Immunogen A synthesized peptide derived from Human Phospho-PRKAA2 (S491)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Monoclonal
Isotype Rabbit IgG
Clone No. 3H10
Purification Method Affinity-chromatography
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form Liquid
Tested Applications ELISA, WB
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Data

Function Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes
Gene References into Functions
  1. PRKAA2 Polymorphisms in rs10789038 and rs2796498 are associated with the susceptibility to type 2 diabetes mellitus and diabetic nephropathy. PMID: 28322508
  2. AMPK phosphorylates DNMT1, RBBP7, and HAT1 and increases interactions of DNMT1, RBBP7, and HAT1. PMID: 28143904
  3. PGC-1alpha protein was higher after HIHVT than after SIT (p < 0.05). Moreover, the AMPKpTHR172/AMPK ratio increased at post after SIT (p < 0.05), whereas this effect was delayed after HIHVT as it increased after 3 h PMID: 28973039
  4. TNF-alpha treatment of colonic rho(0) cells augmented IL-8 expression by 9-fold (P < 0.01) via NF-kappaB compared to TNF-alpha-treated control. Moreover, reduced mitochondrial function facilitated TNF-alpha-mediated NF-kappaB luciferase promoter activity as a result of lowered inhibitory IkappaBalpha (nuclear factor of kappa light polypeptide gene enhancer in B-cell inhibitor, alpha), leading to elevated NF-kappaB. ... PMID: 28183804
  5. Results highlight the contribution of AMPKalpha2 as a mechanism for controlling bladder cancer growth by regulating proliferation through mTOR suppression and induction of p27 protein levels, thus indicating how AMPKalpha2 loss may contribute to tumorigenesis. PMID: 27638620
  6. AMPK phosphorylation of cortactin followed by SIRT1 deacetylation modulates the interaction of cortactin and cortical-actin in response to shear stress. Functionally, this AMPK/SIRT1 coregulated cortactin-F-actin dynamics is required for endothelial nitric oxide synthase subcellular translocation/activation and is atheroprotective. PMID: 27758765
  7. inactivation of AMPKalpha2, but not AMPKalpha1, abrogates the tumor attenuation caused by UBE2O loss. PMID: 28162974
  8. Our findings demonstrate that the AMPKalpha2 catalytic subunit in Kiss1 cells is dispensable for body weight and reproductive function in mice but is necessary for the reproductive adaptations to conditions of acute metabolic distress. PMID: 27732087
  9. Binding of miR-27a to the 3'-UTR of the AMPKalpha2 gene was required to increase the sensitivity of breast cancer cells to metformin. PMID: 27779715
  10. p-AMPKalpha was associated with cervical carcinogenesis, and high expression of AMPKalpha2 was correlated with better disease-free survival in patients with early-stage cervical cancer PMID: 26337566
  11. AMPK-alpha-2 binds to ER-alpha, as well as ER-beta. PMID: 26374855
  12. Data show that activated AMP-activated protein kinase (AMPK) limits retinal pigment epithelial cells (RPE) phagocytic activity by abolishing retinal photoreceptor cell outer segment (POS)-induced activation of c-mer proto-oncogene tyrosine kinase (MerTK). PMID: 26427488
  13. AMPKalpha signalling suppresses EMT and secretion of chemokines in renal tubular epithelia through interaction with CK2beta to attenuate renal injury. PMID: 26108355
  14. A subunit composition of AMPK (alpha2beta2gamma1) is preferred for colorectal cancer cell survival, at least in part, by stabilizing the tumor-specific expression of PGC1B. PMID: 26351140
  15. CaMKKbeta-AMPKalpha2 signaling contributes to mitotic Golgi fragmentation and the G2/M transition in mammalian cells. PMID: 25590814
  16. The basal level of ERK phosphorylation was suppressed in cells treated with siRNA against PRKAA2 (AMPK-knockdown cells). PMID: 25846811
  17. AMPK-alpha co-immunoprecipitates with insulin receptor(IR). The compartmentalization of AMPK-alpha between plasma membrane and Golgi/endosomes fractions in rat's liver is insulin-dependent. Insulin receptor internalization was markedly decreased after AMPK-alpha2 knockdown, and treatment with the ATIC substrate AICAR, which is an allosteric activator of AMPK, increased IR endocytosis in human cultured cells and rat liver. PMID: 25687571
  18. AMPKalpha2 is an essential mediator of nicotine-induced whole-body IR in spite of reductions in adiposity PMID: 25799226
  19. High PRKAA2 expression is associated with follicular lymphoma. PMID: 23396962
  20. AMPKalpha2 suppresses endothelial ACE expression via phosphorylation of p53 and upregulation of microRNA-143/145. PMID: 23476055
  21. WWP1 down-regulates AMPKalpha2 under high glucose culture conditions via the ubiquitin-proteasome pathway PMID: 23293026
  22. underexpression of AMPK is frequently observed in HCC, and that inactivation of AMPK promotes hepatocarcinogenesis by destabilizing p53 in a SIRT1-dependent manner. PMID: 22728651
  23. The results of this study demonistrated that the novel findings that intronic SNPs in the genes coding for the catalytic alpha2 (PRKAA2) of AMPK are associated with antipsychotic-induced weight gain in schizophrenia or schizoaffective disorder patients PMID: 22305490
  24. The AMPK coordinates nutrient status with mitosis completion, which may be critical for the organism's response to low nutrients during development, or in adult stem and cancer cells. PMID: 22137581
  25. AMP activated protein kinase-alpha2 regulates expression of estrogen-related receptor-alpha, a metabolic transcription factor related to heart failure development. PMID: 21825219
  26. IKK is a direct substrate of AMPKalpha2 PMID: 21673972
  27. The crystal structure of the phosphorylated-state mimic T172D mutant kinase domain from the human AMPK alpha2 subunit is reported in the apo form and in complex with a selective inhibitor, compound C. PMID: 21543851
  28. AMPKalpha2-by affecting Fyn phosphorylation and activity-plays a key role in platelet alphaIIbbeta3 integrin signaling, leading to clot retraction and thrombus stability PMID: 20558612
  29. AMPK activates transcription by direct association with chromatin and phosphorylation of histone H2B at ser36; results place AMPK-dependent H2B Ser36 phosphorylation in direct transcriptional and chromatin regulatory pathway leading to stress adaptation PMID: 20647423
  30. Purification and characterization of truncated human AMPK alpha 2 beta 2 gamma 3 heterotrimer from baculovirus-infected insect cells PMID: 19836452
  31. AMPKalpha2 functions as a physiological suppressor of NAD(P)H oxidase, ROS production and 26S proteasome activity in endothelial cells. PMID: 20167927
  32. AMPK functions as a physiological suppressor of endoplasmic reticulum stress by maintaining SERCA activity and intracellular Ca2+ homeostasis. PMID: 20124121
  33. Hepatic amino acid-dependent signaling is under the control of AMP-dependent protein kinase. PMID: 12067722
  34. AMPK signaling is not a key regulatory system of muscle substrate combustion during prolonged exercise and marked activation of AMPK via phosphorylation is not sufficient to maintain an elevated ACCbeta Ser(221) phosphorylation during prolonged exercise PMID: 12413941
  35. Regulation of channel gating by AMP-activated protein kinase modulates cystic fibrosis transmembrane conductance regulator activity in lung submucosal cells. PMID: 12427743
  36. Overexpression of mutant AMPK-alpha1 enhanced forskolin-stimulated I short-circuit currents, consistent with dominant-negative reduction in inhibition of CFTR by endogenous AMPK. PMID: 12519745
  37. results suggest that nuclear translocation of 5' AMP-activated protein kinase might mediate the effects of exercise on skeletal muscle gene and protein expression PMID: 12663462
  38. endothelial AMPK signaling may be a critical determinant of blood vessel recruitment to tissues that are subjected to ischemic stress. PMID: 12788940
  39. LKB1 kinase, which is associated with Peutz-Jeghers cancer-susceptibility syndrome, phosphorylates and activates AMPK in vitro. PMID: 12847291
  40. Acetyl-CoA carboxylase beta phosphorylation is especially sensitive to exercise and tightly coupled to AMPK signaling and that AMPK activation does not depend on AMPK kinase activation during exercise. PMID: 12941758
  41. This study demonstrates that protein content and basal AMPK activity in human skeletal muscle are highly susceptible to endurance exercise training. PMID: 14613924
  42. AMPK and eEF2 kinase may provide a key link between cellular energy status and the inhibition of protein synthesis, a major consumer of metabolic energy PMID: 14709557
  43. AMPK is a major regulator of skeletal muscle HSL activity that can override beta-adrenergic stimulation PMID: 15231718
  44. AMPK is a novel and critical component of HIF-1 regulation, implying its involvement in vanadate-induced prostate carcinogenesis PMID: 15297373
  45. Results indicate that AMP-activated protein kinase (AMPK)alpha negatively regulates acetyl-CoA carboxylase activity and hepatic lipid content. PMID: 15371448
  46. AMPK has a role in the phenobarbital induction of CYP2B gene expression PMID: 15572372
  47. There is a significant basal activity and phosphorylation of AMPK in LKB1-deficient cells that can be stimulated by Ca2+ ionophores, and studies using the CaMKK inhibitor STO-609 and isoform-specific siRNAs show that CaMKKbeta is required for this effect PMID: 16054095
  48. Overexpression of CaMKKbeta in mammalian cells increases AMPK activity, whereas pharmacological inhibition of CaMKK, or downregulation of CaMKKbeta using RNA interference, almost completely abolishes AMPK activation PMID: 16054096
  49. causes inappropriate AMP kinase activation, which leads to glycogen accumulation and heart conduction system disease when transfected into mice PMID: 16275868
  50. data suggest that moderate endurance exercise promotes glucose transport, GLUT4 expression, and insulin sensitivity in skeletal muscle at least partially via activation of the alpha2 isoform of AMPK PMID: 16483872

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Subcellular Location Cytoplasm, Nucleus
Protein Families Protein kinase superfamily, CAMK Ser/Thr protein kinase family, SNF1 subfamily
Database Links

HGNC: 9377

OMIM: 600497

KEGG: hsa:5563

STRING: 9606.ENSP00000360290

UniGene: Hs.437039

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