Code | CSB-RA018994A397phHU |
Size | US$210 |
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Application | Recommended Dilution |
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WB | 1:500-1:5000 |
CUSABIO cloned PTK2 antibody-coding genes into plasma vectors and then transfected these vector clones into mammalian cells using a lipid-based transfection reagent. Following transient expression, the recombinant antibodies against PTK2 were harvested and characterized. The recombinant PTK2 antibody was purified by affinity-chromatography from the culture medium. It can be used to detect PTK2 protein from Human in the ELISA, WB.
PTK2 encodes a cytoplasmic protein tyrosine kinase that was found to be concentrated in focal adhesions formed between cells with components of the extracellular matrix. Diseases associated with PTK2 include malignant astrocytoma and ovarian cancer. Its related pathways include the NF-kappaB pathway and cytokine signaling in the immune system. According to some studies, PTK2 may have the following features.
PTK2 promotes the cancer stem cell signature of hepatocellular carcinoma by activating Wnt/β-catenin signaling. MiR-520d-5p inhibits CC cell proliferation, invasion and migration by targeting PTK2. PTK2 and EIF3S3 genes may be amplified targets of 8q23-q24 and are associated with large hepatocellular carcinoma. Moving and resting actin filaments are involved in the spreading of PtK2 cells after mitosis.
Applications : Western blotting
Sample type: cells
Review: Western blotting of focal adhesion-related signaling proteins.As representative kinases that might stimulate the focal adhesion signal to osteogenesis-related signal, the expression and phosphorylation of FAK, MEK, and ERK were examined. When FAK, MEK, and ERK phosphorylation levels were evaluated, it was found that GO groups—especially the GO-1.7 group—had greater levels than the control group.
By Anonymous