Phospho-PTPN11 (Y542) Antibody

Datasheet
Code CSB-RA019025A542phHU
Size US$350
Uniprot No. Q06124
Image
  • Western Blot
    Positive WB detected in:Hela whole cell lysate,293 whole cell lysate,A549 whole cell lysate(treated with Pervanadate or not)
    All lanes:Phospho-PTPN11 antibody at 0.65μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 68 KDa
    Observed band size: 68 KDa

  • Immunoprecipitating Phospho-PTPN11 in Hela whole cell lysate treated with Pervanadate
    Lane 1: Rabbit control IgG(1μg)instead of CSB-RA019025A542phHU in Hela whole cell lysate treated with Pervanadate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
    Lane 2: CSB-RA019025A542phHU(3μg)+ Hela whole cell lysate treated with Pervanadate(1mg)
    Lane 3: Hela whole cell lysate treated with Pervanadate(20μg)

Immunogen A synthesized peptide derived from Human Phospho-PTPN11 (Y542)
Species Reactivity Human
Tested Applications ELISA, WB, IP; Recommended dilution: WB:1:500-1:5000, IP:1:200-1:1000
Relevance Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Positively regulates MAPK signal transduction pathway (PubMed:28074573). Dephosphorylates GAB1, ARHGAP35 and EGFR (PubMed:28074573). Dephosphorylates ROCK2 at 'Tyr-722' resulting in stimulatation of its RhoA binding activity. Dephosphorylates CDC73 (PubMed:26742426).
Form Liquid
Conjugate Non-conjugated
Storage Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal
Alias Tyrosine-protein phosphatase non-receptor type 11, Protein-tyrosine phosphatase 1D, PTP-1D, Protein-tyrosine phosphatase 2C, PTP-2C, SH-PTP2, SHP-2, Shp2, SH-PTP3, PTPN11, PTP2C, SHPTP2
Immunogen Species Homo sapiens (Human)
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunoprecipitation (IP) Protocol
Research Area Signal Transduction
Gene Names PTPN11
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Positively regulates MAPK signal transduction pathway
Involvement in disease LEOPARD syndrome 1 (LPRD1); Noonan syndrome 1 (NS1); Leukemia, juvenile myelomonocytic (JMML); Metachondromatosis (MC)
Subcellular Location Cytoplasm, Nucleus
Protein Families Protein-tyrosine phosphatase family, Non-receptor class 2 subfamily
Tissue Specificity Widely expressed, with highest levels in heart, brain, and skeletal muscle.
Database Links

HGNC: 9644

OMIM: 151100

KEGG: hsa:5781

STRING: 9606.ENSP00000340944

UniGene: Hs.506852

Pathway Jak-STAT signaling pathway
Ras signaling pathway
Leukocyte transendothelial migration
Natural killer cell mediated cytotoxicity
Adipocytokine signaling pathway
Phospholipase D signaling pathway
Neurotrophin signaling pathway

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