Code | CSB-RA289762A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IHC | 1:50-1:200 |
The PTPN11 recombinant monoclonal antibody is produced using protein and DNA recombinant technology. Initially, a synthetic peptide derived from human PTPN11 was used to immunize mice. After a certain period, the spleen of mice was removed under aseptic conditions, and total RNA from spleen cells was extracted. cDNA synthesized from RNA reverse transcription was used as a template to amplify the PTPN11 antibody gene by PCR. The obtained PTPN11 antibody gene was inserted into a vector and transfected into host cells for culture. The PTPN11 recombinant monoclonal antibody was purified from the cell culture supernatant using affinity chromatography and rigorously verified for human PTPN11 protein detection in ELISA, WB, and IHC experiments.
The PTPN11 protein mainly regulates cell signaling pathways by controlling the phosphorylation status of specific tyrosine residues in target proteins. PTPN11 acts as a phosphatase enzyme, removing phosphate groups from tyrosine residues in target proteins, which in turn modulates their activity and function. PTPN11 is particularly involved in the regulation of several important signaling pathways, including those mediated by growth factors, cytokines, and hormones. By dephosphorylating target proteins, PTPN11 plays a crucial role in controlling cellular processes such as cell growth, differentiation, and survival. Mutations in the PTPN11 gene have been associated with various human diseases, including developmental disorders and certain types of cancer.
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