Code | CSB-RA779218A0HU |
Size | US$210 |
Order now | |
Image |
|
Have Questions? | Leave a Message or Start an on-line Chat |
Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
CUSABIO produced the PTPN11 recombinant monoclonal antibody using protein technology and DNA recombinant technology. Initially, mice were immunized with a synthesized peptide derived from human SHP2, and after a certain period of time, their spleen was removed under aseptic conditions. The total RNA of the spleen cells was extracted, and the cDNA synthesized by RNA reverse transcription was used as a template for PCR amplification of the PTPN11 antibody gene. The obtained gene was then introduced into a vector and transfected into host cells for culture. The PTPN11 recombinant monoclonal antibody was purified from the supernatant of the cell culture using affinity chromatography. This antibody was rigorously verified and can be utilized for detecting human PTPN11 protein in ELISA and WB experiments.
The PTPN11 protein, also known as SHP2, is a signaling protein that acts as a phosphatase. It is involved in several signaling pathways in cells, including growth factor signaling and immune response. SHP2 is also involved in immune signaling by regulating the activity of immune cells. In T cells, SHP2 plays a role in regulating T cell receptor signaling, which is critical for the activation and function of T cells. SHP2 has also been shown to regulate the activity of macrophages and other immune cells. Mutations in the PTPN11 gene can lead to several diseases, including Noonan syndrome, LEOPARD syndrome, and some types of leukemia.
There are currently no reviews for this product.