Alternative Names
Drosophila, homolog of, MADR2 antibody; hMAD-2 antibody; HsMAD2 antibody; JV18 antibody; JV18-1 antibody; JV181 antibody; MAD antibody; MAD homolog 2 antibody; MAD Related Protein 2 antibody; Mad-related protein 2 antibody; MADH2 antibody; MADR2 antibody; MGC22139 antibody; MGC34440 antibody; Mother against DPP homolog 2 antibody; Mothers against decapentaplegic homolog 2 antibody; Mothers against decapentaplegic, Drosophila, homolog of, 2 antibody; Mothers against DPP homolog 2 antibody; OTTHUMP00000163489 antibody; Sma and Mad related protein 2 antibody; Sma- and Mad-related protein 2 MAD antibody; SMAD 2 antibody; SMAD family member 2 antibody; SMAD, mothers against DPP homolog 2 antibody; SMAD2 antibody; SMAD2_HUMAN antibody
Immunogen
A synthesized peptide derived from Human Phospho-SMAD2 (S250)
Immunogen Species
Homo sapiens (Human)
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Tested Applications
ELISA, WB
Recommended Dilution
Application |
Recommended Dilution |
WB |
1:500-1:5000 |
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description
The phospho-SMAD2 (S250) recombinant monoclonal antibody is produced using advanced techniques in protein and DNA recombinant technology. Initially, animals are immunized with a synthetic peptide derived from human phospho-SMAD2 (S250), resulting in the generation of B cells. From these B cells, positive clones are carefully selected and identified. The genes encoding the phospho-SMAD2 (S250) antibody are then amplified through PCR and inserted into a plasmid vector, creating a recombinant vector. This recombinant vector is transfected into host cells to enable the expression of the phospho-SMAD2 (S250) antibody. The phospho-SMAD2 (S250) recombinant monoclonal antibody is subsequently purified from the cell culture supernatant using affinity chromatography. This phospho-SMAD2 (S250) recombinant monoclonal antibody offers a reliable means to detect human phospho-SMAD2 (S250) protein with precision and accuracy in ELISA and WB applications.
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.