Code | CSB-RA618017A250phHU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
The phospho-SMAD2 (S250) recombinant monoclonal antibody is produced using advanced techniques in protein and DNA recombinant technology. Initially, animals are immunized with a synthetic peptide derived from human phospho-SMAD2 (S250), resulting in the generation of B cells. From these B cells, positive clones are carefully selected and identified. The genes encoding the phospho-SMAD2 (S250) antibody are then amplified through PCR and inserted into a plasmid vector, creating a recombinant vector. This recombinant vector is transfected into host cells to enable the expression of the phospho-SMAD2 (S250) antibody. The phospho-SMAD2 (S250) recombinant monoclonal antibody is subsequently purified from the cell culture supernatant using affinity chromatography. This phospho-SMAD2 (S250) recombinant monoclonal antibody offers a reliable means to detect human phospho-SMAD2 (S250) protein with precision and accuracy in ELISA and WB applications.
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