Code | CSB-RA618017A255phHU |
Size | US$210 |
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Application | Recommended Dilution |
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IF | 1:20-1:200 |
CUSABIO developed the phospho-SMAD2 (S255) recombinant monoclonal antibody using protein technology and DNA recombinant techniques. The first step is to obtain the phospho-SMAD2 (S255) antibody gene. Immunizing an animal with a synthesized peptide derived from human phospho-SMAD2 (S255) and isolating B cells. These B cells are then screened to isolate positive ones, followed by single clone identification. Next, the light and heavy chains of the phospho-SMAD2 (S255) antibody are amplified via PCR and integrated into a plasmid vector to construct a recombinant vector. This recombinant vector is subsequently transfected into host cells to facilitate antibody expression. The phospho-SMAD2 (S255) recombinant monoclonal antibody is purified from the supernatant of cell culture using affinity chromatography. Finally, stringent validation is conducted to ensure its accuracy and efficacy for the detection of human SMAD2 protein phosphorylated at S255 residue in ELISA and IF applications.
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