Alternative Names
Drosophila, homolog of, MADR2 antibody; hMAD-2 antibody; HsMAD2 antibody; JV18 antibody; JV18-1 antibody; JV181 antibody; MAD antibody; MAD homolog 2 antibody; MAD Related Protein 2 antibody; Mad-related protein 2 antibody; MADH2 antibody; MADR2 antibody; MGC22139 antibody; MGC34440 antibody; Mother against DPP homolog 2 antibody; Mothers against decapentaplegic homolog 2 antibody; Mothers against decapentaplegic, Drosophila, homolog of, 2 antibody; Mothers against DPP homolog 2 antibody; OTTHUMP00000163489 antibody; Sma and Mad related protein 2 antibody; Sma- and Mad-related protein 2 MAD antibody; SMAD 2 antibody; SMAD family member 2 antibody; SMAD, mothers against DPP homolog 2 antibody; SMAD2 antibody; SMAD2_HUMAN antibody
Immunogen
A synthesized peptide derived from Human Phospho-SMAD2 (S255)
Immunogen Species
Homo sapiens (Human)
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Tested Applications
ELISA, IF
Recommended Dilution
Application |
Recommended Dilution |
IF |
1:20-1:200 |
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description
CUSABIO developed the phospho-SMAD2 (S255) recombinant monoclonal antibody using protein technology and DNA recombinant techniques. The first step is to obtain the phospho-SMAD2 (S255) antibody gene. Immunizing an animal with a synthesized peptide derived from human phospho-SMAD2 (S255) and isolating B cells. These B cells are then screened to isolate positive ones, followed by single clone identification. Next, the light and heavy chains of the phospho-SMAD2 (S255) antibody are amplified via PCR and integrated into a plasmid vector to construct a recombinant vector. This recombinant vector is subsequently transfected into host cells to facilitate antibody expression. The phospho-SMAD2 (S255) recombinant monoclonal antibody is purified from the supernatant of cell culture using affinity chromatography. Finally, stringent validation is conducted to ensure its accuracy and efficacy for the detection of human SMAD2 protein phosphorylated at S255 residue in ELISA and IF applications.
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.