Phospho-SMAD2 (S255) Antibody

Datasheet
Code CSB-RA618017A255phHU
Size US$350
Uniprot No. Q15796
Image
  • Immunofluorescence staining of A549 cells with CSB-RA618017A255phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Immunogen A synthesized peptide derived from Human Phospho-SMAD2 (S255)
Species Reactivity Human
Tested Applications ELISA, IF; Recommended dilution: IF:1:20-1:200
Relevance Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal
Alias Mothers against decapentaplegic homolog 2, MAD homolog 2, Mothers against DPP homolog 2, JV18-1, Mad-related protein 2, hMAD-2, SMAD family member 2, SMAD 2, Smad2, hSMAD2, SMAD2, MADH2, MADR2
Immunogen Species Homo sapiens (Human)
Protocols ELISA Protocol
Immunofluorescence (IF) Protocol
Research Area Signal Transduction
Gene Names SMAD2
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Subcellular Location Cytoplasm, Nucleus
Protein Families Dwarfin/SMAD family
Tissue Specificity Expressed at high levels in skeletal muscle, endothelial cells, heart and placenta.
Database Links

HGNC: 6768

OMIM: 601366

KEGG: hsa:4087

STRING: 9606.ENSP00000262160

UniGene: Hs.12253

Pathway Hippo signaling pathway
TGF-beta signaling pathway
Adherens junction
Cell cycle
Cellular senescence
Signaling pathways regulating pluripotency of stem cells
Th17 cell differentiation
AGE-RAGE signaling pathway in diabetic complications
Apelin signaling pathway
FoxO signaling pathway
Endocytosis

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