RBM3 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA253160A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: Jurkat whole cell lysate(30µg) All lanes:RBM3 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 17 kDa Observed band size: 17 kDa Exposure time：2min IHC image of CSB-RA253160A0HU diluted at 1:100 and staining in paraffin-embedded human thyroid tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of Hela cell with CSB-RA253160A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing jurkat cells stained with CSB-RA253160A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Target Background

Product Details

Uniprot No.

P98179

Target Names

RBM3

Alternative Names

RNA-binding protein 3 (RNA-binding motif protein 3) (RNPL), RBM3, RNPL

Species Reactivity

Human

Immunogen

A synthesized peptide from human RBM3 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

1D4

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

RBM3 (RNA-binding motif protein 3) is a cold-inducible RNA-binding protein that plays a critical role in cellular stress responses, particularly under conditions of hypothermia and hypoxia. This protein has garnered significant research interest for its neuroprotective properties and its involvement in regulating mRNA stability and translation during cellular stress. RBM3 expression patterns have also emerged as relevant markers in cancer biology, where altered levels may influence tumor progression and patient outcomes.

This recombinant monoclonal antibody, clone 1D4, offers researchers the reproducibility and consistency that comes with sequence-defined production. Unlike traditional hybridoma-derived antibodies, recombinant technology ensures that every lot performs identically, eliminating the variability that can compromise longitudinal studies or multi-site collaborations. The rabbit IgG format provides excellent signal-to-noise characteristics across diverse experimental platforms.

Validation testing demonstrates reliable performance across multiple applications, giving researchers flexibility in their experimental design. Western blot analysis in Jurkat whole cell lysate detects a clean band at the predicted 17 kDa molecular weight, confirming target specificity at dilutions between 1:500 and 1:2000. Immunohistochemistry staining in paraffin-embedded human thyroid tissue reveals clear localization patterns, while immunofluorescence studies in HeLa cells enable subcellular visualization of RBM3 distribution. Flow cytometry validation in Jurkat cells further expands utility for quantitative single-cell analysis.

The antibody is supplied in a glycerol-containing buffer optimized for long-term stability at -20°C or -80°C. This RBM3 antibody serves researchers investigating stress response mechanisms, hypothermia-induced neuroprotection, and cancer biomarker studies where consistent, high-quality detection is essential for meaningful results.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Cold-inducible mRNA binding protein that enhances global protein synthesis at both physiological and mild hypothermic temperatures. Reduces the relative abundance of microRNAs, when overexpressed. Enhances phosphorylation of translation initiation factors and active polysome formation.

Gene References into Functions

Results showed that high RBM3 expression in gastric cancer is mainly found in intestinal-type of Lauren grade and is associated with longer overall survival time. PMID: 29263314
Overexpression of RBM3 rescued SH-SY5Y cells from UV-induced apoptosis. PMID: 28831692
RBM3 is overexpressed in bipolar disorder patients responding to lithium treatment compared to non-responders. PMID: 28616776
Loss of RBM3 expression is an unfavourable prognostic marker in colorectal cancers (CRCs), and is linked to right-sided tumour localization. PMID: 25922889
High RBM3 expression is an independent predictor of prolonged survival in metastatic colorectal cancer patients. PMID: 28800641
Results show that RBM3 overexpression results in increased stemness in colon cancer cells, and inactivation of GSK3 through phosphorylation, thereby enhancing b-catenin signaling activity the colorectal cancer cells. PMID: 26331352
Low RBM3 expression is associated with colon Cancer. PMID: 28373441
Studies showed RBM3 as one of the important cold shock protein with critical roles in rapid cell adaption to the alterations of the environmental stress. Also, RBM3 plays an important role in neuroprotection, anti-apoptotic functions, cell proliferation as well as its function as proto-oncogene.[review] PMID: 27364162
The results indicate the existence of a negative feedback loop that regulates fever via reduced RBM3 levels and increased expression of miR-142-5p and miR-143. PMID: 26825461
Low RBM3 immunoexpression is associated with urothelial carcinoma of the bladder. PMID: 26577765
RBM3 may be a potential biomarker for treatment stratification in patients with metastatic non-seminomatous germ cell tumours. PMID: 25811459
High RBM3 expression is an independent prognostic marker in prostate cancer. PMID: 24380696
The data suggest that the overexpression of RBM3 may serve as an important molecular mechanism underlying astrocytic carcinogenesis. PMID: 23673116
A novel role of RBM3 in linking stress-regulated RNA splicing to tumorigenesis. PMID: 23667174
Loss of RBM3 expression is associated with more aggressive tumors and poorer prognosis of urothelial bladder cancer. Findings may indicate that loss of RBM3 expression results in a phenotype more prone to metastatic spread than local aggressiveness. PMID: 23565664
the inverse association and prognostic impact of MCM3 and RBM3 expression indicate a possible interaction of these proteins in melanoma progression PMID: 22805320
high tumor-specific nuclear expression of RBM3 is an independent predictor of good prognosis in colorectal cancer PMID: 21956899
high nuclear expression of RBM3 in prostate cancer is associated with a prolonged time to disease progression PMID: 21955582
RBM3 is down-regulated in metastatic melanoma and high nuclear RBM3 expression in the primary tumour is an independent marker of a prolonged overal survival. PMID: 21777469
RBM3 may be a useful prognostic and treatment predictive marker in epithelial ovarian cancer. PMID: 20727170
RBM3 is a critical factor providing cellular survival advantages in an adverse microenvironment presumably by restoring translation efficacy PMID: 19770690
Nuclear RBM3 is an independent favorable prognostic factor in breast cancer, and seems to have a specific role in estrogen receptor-positive tumors. PMID: 19734850
RBM3 and CIRP are adaptatively expressed in response to hypoxia by a mechanism that involves neither HIF-1 nor mitochondria PMID: 15075239
From these results, it seems that the X-chromosome, through its RBM genes, plays a formerly unknown role in the regulation of programmed cell death (apoptosis) in breast cancer. PMID: 16552754
the RNA stabilizing and translation regulatory protein RBM3 is a novel proto-oncogene that induces transformation when overexpressed and is essential for cells to progress through mitosis. PMID: 18427544
pharmacological modulation of RBM3 and CIRBP may represent novel therapeutic approaches for prostate cancer. PMID: 19277990

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Subcellular Location

Nucleus. Cytoplasm. Cell projection, dendrite.

Database Links

HGNC: 9900

OMIM: 300027

KEGG: hsa:5935

STRING: 9606.ENSP00000365946

UniGene: Hs.301404

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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