Code | CSB-RA149196A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IHC | 1:50-1:200 |
The SMC1A recombinant monoclonal antibody was produced using protein technology and DNA recombinant technology. Initially, mice were immunized with a synthetic peptide derived from human SMC1A, following which the spleen of mice was extracted aseptically. The total RNA of spleen cells was isolated, and cDNA synthesized by RNA reverse transcription was used as the template for PCR amplification of the SMC1A antibody gene. Subsequently, the obtained gene was introduced into a vector and then transfected into host cells for culture. The SMC1A recombinant monoclonal antibody was purified from the supernatant of the cell culture using affinity chromatography. It was subjected to rigorous validation and can be employed for detecting human SMC1A protein in ELISA, WB, and IHC experiments.
The SMC1A protein is a component of the cohesin complex and plays a role in chromosome organization and DNA repair. SMC1A forms a ring-like structure around the DNA, which helps to stabilize the interaction between the sister chromatids. SMC1A functions in DNA repair by facilitating the rejoining of DNA double-strand breaks. SMC1A has been implicated in the regulation of gene expression. It has been shown to interact with transcription factors and other chromatin-associated proteins to control the transcription of specific genes.
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