Product Details

Uniprot No.

Target Names

Alternative Names

Structural maintenance of chromosomes protein 1A (SMC protein 1A) (SMC-1-alpha) (SMC-1A) (Sb1.8), SMC1A, DXS423E KIAA0178 SB1.8 SMC1 SMC1L1

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human SMC1

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

8C11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

SMC1A serves as a core component of the cohesin complex, playing essential roles in chromosome cohesion during cell division, DNA repair pathways, and genome stability maintenance. This protein's involvement in sister chromatid cohesion and its participation in the DNA damage response make it a critical target for researchers investigating cell cycle regulation, chromosomal dynamics, and cancer biology.

This recombinant monoclonal antibody, generated from clone 8C11, offers the reproducibility and consistency that demanding experimental workflows require. Because the antibody sequence is defined and produced recombinantly in rabbit host cells, researchers can expect reliable performance across experiments and between lots, eliminating the variability often encountered with traditional hybridoma-derived antibodies. The affinity-purified preparation ensures high specificity for your target.

Validation studies demonstrate robust performance across multiple applications. In western blot analysis, this antibody detects SMC1A at the expected molecular weight of 144 kDa across a diverse panel of human cell lines including HeLa, K562, 293T, Jurkat, A549, HL60, and MCF-7, with recommended dilutions ranging from 1:500 to 1:5000. This broad detection across different cellular contexts confirms reliable recognition of endogenous SMC1A protein. For tissue-based studies, immunohistochemistry validation in paraffin-embedded human ovarian cancer and glioma samples shows clear nuclear staining patterns consistent with SMC1A's known localization, using dilutions between 1:50 and 1:200.

Whether you're exploring cohesin complex dynamics, investigating chromosomal abnormalities in cancer models, or studying DNA damage response mechanisms, this antibody provides a dependable tool for epigenetics, nuclear signaling, and cell biology research applications.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.

Gene References into Functions

Both the SMC1A and SMC3 gene mutation tests were negative in all Chinese patients with Cornelia de Lange syndrome. PMID: 29452578
maintenance of the cancer cell state is dependent on recruitment of Mediator and Cohesin through FOXA and master transcription factors PMID: 27739523
We conclude that SMC1A variants can result in a phenotype resembling CdLS and a phenotype resembling Rett syndrome. Resemblances between the SMC1A group and the NIPBL group suggest that a disturbed cohesin function contributes to the phenotype, but differences between these groups may also be explained by other underlying mechanisms such as moonlighting of the cohesin genes. PMID: 28548707
All the nine probands with syndromic craniosynostosis were found to carry the possibly causative variants, among which three variants including two missense mutations in IFT122 gene, in SMC1A gene and a frameshift mutation in TWIST1 gene have never been reported in patients before. PMID: 29037998
Based on these findings, LVNC cardiomyopathy and cleft lip should be considered features of SMC1A-associated CdLS. All patients should receive echocardiogram and undergo thorough ophthalmologic evaluation as part of routine CdLS care. PMID: 28102598
Elevated expression of SMC1A in colorectal cancer cells promoted liver metastasis by recruiting the circulating tumor-associated fibroblasts. PMID: 27826041
This study demonstrated that Truncation mutations in SMC1A cause a severe epilepsy phenotype with cluster seizures in females. PMID: 28166369
Our data show the existence of a novel phenotypic entity - distinct from Cornelia de Lange syndrome - and caused by de novo SMC1A loss-of-function mutations PMID: 26752331
We identified a large number of mutations in the CC region of both Smc1 and Smc3... we introduced them to the yeast Smc1 and Smc3 CC domains and characterized the effect of these mutant alleles on cohesin's function. We identified a missense mutation in the region of the kink domain of Smc3, which was previously identified in kidney carcinoma. PMID: 27307603
High SMC1A expression is associated with prostate cancer. PMID: 27667360
Results showed that the high expression of SMC1 often promoted epithelial-mesenchymal transition, accompanied by the enhanced expression of Brachyury in triple-negative breast cancer cells. PMID: 26781859
SMC1A plays an oncogenic role in colorectal cancer. PMID: 26637483
Loss-of-function mutations of SMC1A may be associated with early-onset encephalopathy with epilepsy. PMID: 26358754
numerous dysregulated genes occupied by cohesin by combining the transcriptome of CdLS cell lines carrying mutations in SMC1A gene, were identified. PMID: 26581180
two novel de novo heterozygous frameshift mutations in the SMC1A gene were identified in two patients with developmental delay and epilepsy. PMID: 26386245
Our findings identify both SMC1 and CTCF as critical regulators of the differentiation-dependent life cycle of high-risk human papillomaviruses PMID: 25875106
same down-regulation of cohesin targets is observed in SMC1A-mutated patient fibroblasts PMID: 26206533
Results show that SMC1A is overexpressed in colorectal cancer tissues and correlated with poor prognosis for late stage disease. PMID: 25884313
The SMC1a mutation leads to chromosomal instability and tumorigenesis in early colorectal adenomas. PMID: 25080505
A dominant negative effect is considered the pathogenic mechanism in SMC1A-defective female patients, the level of allelic preferential expression might be one of the factors contributing to the wide phenotypic variability observed in these patients. PMID: 24756084
Clinical comparison between our patient with a previously reported individual with a SMC1A duplication and four male carriers of similar sSMC reported in databases, suggest that they all share clinical features related to cohesinopathies PMID: 23683030
The mutation c.1731G>A/p.E577E in our patient expands the mutational spectrum of SMC1A to splice site mutations and also represents the first exonic synonymous splice site mutation observed in any human cohesinopathy PMID: 23863341
Our clinical and molecular findings expand the total number of characterized SMC1A-mutated patients (from 44 to 52) and the restricted repertoire of SMC1A mutations (from 29 to 34), contributing to the molecular and clinical signature of SMC1A-based CdLS. PMID: 24124034
Inhibiting SMC1A expression efficiently (P < 0.001) resulted in inhibiting the proliferation and colony formation of U251 and U87MG glioblastoma cells. PMID: 23754617
Human SMC3 knock-down rendered SMC1 instable without cytoplasmic accumulation. PMID: 23776448
studies report for the first time that SMC1 is overexpressed in TNBC cells where it plays a role in cell migration and drug sensitivity, and thus provides a potential therapeutic target for this highly invasive breast cancer subtype PMID: 23717600
These results suggest that SMC1A upregulation is involved in the pathogenesis of glioma. PMID: 23638217
c-MYC down-regulation caused by cohesin mutations in SMC1A and SMC3 genes may be an early/primary event in the pathogenesis of Cornelia de Lange syndrome. PMID: 23106691
NIPBL, SMC1A, and SMC3 mutation-positive patients were equally likely to have congenital heart diseases in Cornelia de lange syndrome. PMID: 22965847
phosphorylation of Rad50 plays a key regulatory role as an adaptor for specific ATM-dependent downstream signaling through SMC1 for DNA repair and cell cycle checkpoint control in the maintenance of genome integrity. PMID: 21757780
phosphorylation of SMC1 is required for an increased mobility after DNA damage in G2-phase cells, suggesting that ATM-dependent phosphorylation facilitates mobilization of the cohesin complex after DNA damage PMID: 21056556
SMC1A missense mutation is associated with Cornelia de Lange syndrome. PMID: 20635401
Low SMC1A expression predicts poor survival in acute myeloid leukemia. PMID: 20514443
The identification of 14 additional mutations of the cohesin complex genes NIPBL and SMC1A in a cohort of 30 unrelated patients with Cornelia de Lange syndrome, is reported. PMID: 20358602
Interaction between Rae1 and cohesin subunit SMC1 is required for proper spindle formation. PMID: 20016259
results suggest that mechanistically SMC1A-related Cornelia de Lange Syndrome is not due to altered levels of the SMC1A transcript, but rather that the mutant proteins maintain a residual function in males and enact a dominant negative effect in females. PMID: 19701948
This protein is localized at the kinetochores and is involved in cell division. PMID: 12199140
Inhibition of SMC1 is associated with chromosomal aberrations PMID: 15640246
RPGR-ORF15, which is mutated in retinitis pigmentosa, associates with SMC1. PMID: 16043481
Replication timing of FRA3B in G2 was studied by bromodeoxyuridine (BrdU) labeling by a fluorescence in situ hybridization (FISH)-based approach through the analysis of clones spanning the FRA3B region. PMID: 16242161
mutations in SMC1L1 (also known as SMC1), which encodes a different subunit of the cohesin complex, are responsible for Cornelia de Lange syndrome in three male members of an affected family and in one sporadic case PMID: 16604071
SMC1 binding represses OARE [OA (okadaic acid) response element] activity and its dissociation allows the recruitment of CAR(constitutive active/androstane receptor) to the OARE, synergizing the expression of the CYP2B6 gene. PMID: 16623664
nuclear exclusion is important to prevent cohesin cleavage during interphase in the absence of securin and the phosphorylation inhibition PMID: 17102637
So far, two genes (NIPBL and SMC1L1) have been identified causing Cornelia de Lange syndrome (CdLS) or CdLS-like phenotypes. PMID: 17106445
Mutations in SMC1A cause a mild variant of cornelia de Lange syndrome with predominant mental retardation PMID: 17273969
The S-phase checkpoint, regulated by the ATM-p95/NBS1-SMC1 pathway, was also triggered in hypoxia/reoxygenation-exposed lymphocytes. PMID: 17544403
identified as one of five genes containing 11 somatic mutations in a panel that included 132 colorectal cancers, then demonstrated that down-regulation of such homologs resulted in chromosomal instability and chromatid cohesion defects in human cells PMID: 18299561
ATM plays a fundamental role in promoting the radiation-induced interaction of NBS1 with SMC1 in the presence of BRCA1, leading to the maintenance of chromosomal integrity. PMID: 18763866
SMC1 is recruited to microtubule-bound RNA export factor 1 (Rae1) at the mitotic spindle pole. PMID: 18832153
Cornelia de Lange syndrome mutations in SMC1A or SMC3 bind to DNA with higher affinity and display genomic instability. PMID: 18996922

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Involvement in disease

Cornelia de Lange syndrome 2 (CDLS2)

Subcellular Location

Nucleus. Chromosome. Chromosome, centromere, kinetochore. Note=Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesin complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex. The phosphorylated form on Ser-957 and Ser-966 associates with chromatin during G1/S/G2 phases but not during M phase, suggesting that phosphorylation does not regulate cohesin function. Integral component of the functional centromere-kinetochore complex at the kinetochore region during mitosis.

Protein Families

SMC family, SMC1 subfamily

Database Links

HGNC: 11111

OMIM: 300040

KEGG: hsa:8243

STRING: 9606.ENSP00000323421

UniGene: Hs.211602

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Code	CSB-RA149196A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, 293T whole cell lysate, Jurkat whole cell lysate, A549 whole cell lysate, HL60 whole cell lysate, MCF-7 whole cell lysate All lanes: SMC1A antibody at 1:1500 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 144 kDa Observed band size: 144 kDa IHC image of CSB-RA149196A0HU diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA149196A0HU diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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SMC1A Recombinant Monoclonal Antibody

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