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SMURF1 Recombinant Monoclonal Antibody

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Datasheet
Code CSB-RA875720MA1HU
Size $49.9
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Image
  • Overlay Peak curve showing A431 cells stained with CSB-RA875720MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific at 1:200 dilution for 35min at 4℃.Control antibody (green line) was human IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details

Uniprot No.
Q9HCE7
Target Names
SMURF1
Alternative Names
E3 ubiquitin-protein ligase SMURF1 antibody; hSMURF1 antibody; KIAA1625 antibody; Smad specific E3 ubiquitin ligase 1 antibody; SMAD specific E3 ubiquitin protein ligase 1 antibody; Smad ubiquitination regulatory factor 1 antibody; SMAD-specific E3 ubiquitin-protein ligase 1 antibody; SMUF1_HUMAN antibody; SMURF 1 antibody; smurf1 antibody
Species Reactivity
Human
Immunogen
Recombinant Human SMURF1 protein
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
hIgG1
Clone No.
1A9H4
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, FC
Recommended Dilution
Application Recommended Dilution
FC 1:50-1:200
Protocols
ELISA Protocol
Flow Cytometry (FC) Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

SMURF1 (Smad ubiquitination regulatory factor 1) functions as an E3 ubiquitin-protein ligase that plays a central role in regulating TGF-β and BMP signaling pathways through targeted degradation of Smad proteins. This regulatory activity positions SMURF1 as a critical modulator of cellular processes including bone formation, cell polarity, and epithelial-to-mesenchymal transition, making it a compelling target for researchers investigating developmental biology, cancer progression, and fibrotic diseases.

This recombinant monoclonal antibody, clone 1A9H4, offers the reproducibility and consistency that demanding research applications require. Developed using recombinant technology with a defined sequence, this antibody eliminates the lot-to-lot variability often encountered with traditional hybridoma-derived reagents. The human IgG1 isotype format, combined with affinity chromatography purification, ensures high specificity for your SMURF1 detection needs.

Validation through flow cytometry demonstrates reliable intracellular detection of SMURF1 in human A431 epidermoid carcinoma cells. Using fixed and permeabilized cells, the antibody produces clear signal separation from isotype control at dilutions of 1:50 to 1:200, confirming its utility for quantitative analysis of SMURF1 expression levels in human cell populations. The antibody has also been validated for ELISA applications, providing flexibility across different experimental workflows.

Whether you are investigating ubiquitin-mediated protein degradation, dissecting TGF-β signaling dynamics, or exploring SMURF1's emerging roles in tumorigenesis and metastasis, this recombinant antibody delivers the performance consistency essential for generating reliable, publishable data in your research program.

Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA. Promotes ubiquitination and subsequent proteasomal degradation of MAVS. Plays a role in dendrite formation by melanocytes.
Gene References into Functions
  1. Nedd8 binding to Smurf plays important roles in the regulation of cell migration and the BMP and TGFbeta signaling pathways. PMID: 28169289
  2. Expression of Smurf1 was increased with WHO grade and was consistent with poor prognosis of gliomas. PMID: 28321604
  3. Smurf1 interacts with and targets Securin, an inhibitor of sister-chromatid separation, for poly-ubiquitination and proteasomal degradation. PMID: 28658604
  4. Smurf1 overexpression decreases USP25 protein turnover, and the E3 ligase enzymatic activity of Smurf1 is required for USP25 degradation. PMID: 29518389
  5. SMURF1 can be potentially used as a clinical biomarker and target for novel treatment of human GC. PMID: 28731194
  6. Uev1A appears to be involved in the BMP signaling pathway in which it collaborates with a ubiquitin E3 ligase Smurf1 to promote Smad1 degradation in a Ubc13-independent manner. PMID: 28771228
  7. High smurf1 expression is associated with neoplasms. PMID: 27036023
  8. activation of AMPK upregulated Smad6 and Smurf1 and thereby enhanced their interactions, resulting in its proteosome-dependent degradation of ALK2. PMID: 28847510
  9. we propose that the PKA-Smurf1-PIPKIgamma pathway has an important role in pulmonary tumorigenesis and imposes substantial clinical impact on development of novel diagnostic markers and therapeutic targets for lung cancer treatment. PMID: 28581524
  10. SMURF1 is increased in patients with pulmonary arterial hypertension PMID: 27214554
  11. Data suggest that SMURF1 is required for S phase progression; SMURF1 promotes ubiquitination-dependent degradation of WEE1; these functions of SMURF1 appear to be linked and may be important in cell proliferation and tumorigenesis. (SMURF1 = SMAD specific E3 ubiquitin protein ligase 1; WEE1 = wee 1 homolog [S pombe] protein) PMID: 28294307
  12. Taken together, our study for the first time clarified that the E3 ligase Smurf1 regulates USP5 protein stability and USP5-mediated TNF-alpha production through the ubiquitin proteasome pathway. PMID: 27133717
  13. results reveal the regulatory circuit between RUNX2 and SMURF1 controls RUNX2 expression and regulates odntoblastic differentiation in hDPSCs. PMID: 25260729
  14. EGF/Smurf1 inhibits Wnt/beta-catenin-induced osteogenic differentiation and that Smurf1 downregulates Wnt/b-catenin signaling by enhancing proteasomal degradation of beta-catenin. PMID: 26015066
  15. a model that CD166 regulates MCAM through a signaling flow from activation of PI3K/AKT and c-Raf/MEK/ERK signaling to the inhibition of potential MCAM ubiquitin E3 ligases, betaTrCP and Smurf1. PMID: 26004137
  16. SND1 promoted expression of the E3 ubiquitin ligase Smurf1, leading to RhoA ubiquitination and degradation. PMID: 25596283
  17. NF-kappaB bind to the -411 to -420 region of the SMURF1 promoter and plays an essential role in the expression of SMURF1. PMID: 26252093
  18. Smurf1 determines cell apoptosis rates downstream of DNA damage-induced ATR/Chk1 signalling by promoting degradation of RhoB. PMID: 25249323
  19. The role of SMURF1 and inhibition of BMP signaling in maintaining a CSC-like population was confirmed by the loss of a CD44(high) expressing. PMID: 25471937
  20. Results suggest that elevated transcription and expression levels of ubiquitin ligase E3s WWP1, Smurf1 and Smurf2 genes may be the mechanisms of occurrence, development and metastasis of prostate cancer. PMID: 25051198
  21. Fbxo3 promotes the proteasomal degradation of Smurf1. Fbxo3 promotes the poly-ubiquitination of Smurf1. PMID: 25721664
  22. TRIB2 associated-ubiquitin E3 ligases beta-transducin repeat-containing E3 ubiquitin protein ligase (beta-TrCP), COP1 and Smad ubiquitination regulatory factor 1 (Smurf1) reduced TCF4/beta-Catenin expression, and these effects could be enhanced by TRIB2. PMID: 25311538
  23. association of cortactin with Pfn-1 is regulated by c-Abl-mediated cortactin phosphorylation PMID: 24700460
  24. Smurf1 is localized within the Hirano bodies of Alzheimer's disease brains. PMID: 24238996
  25. CKIP-1 controlled Smurf1 expression in colon cancer PMID: 23995790
  26. Inhibiting Smurf1 mediated ubiquitination of Smad1/5. PMID: 24828823
  27. Results suggest that EGF-induced SMURF1 plays a role in breast cancer cell migration and invasion through the downregulation of RhoA. PMID: 24241683
  28. impaired phosphorylation and ubiquitination by p70S6K and Smurf1 increase the protein stability of TRIB2 in liver cancer PMID: 24089522
  29. Data indicate that Smad ubiquitin regulatory factor Smurf1 regulates cell migration through ubiquitination of tumor necrosis factor receptor-associated factor 4 (TRAF4). PMID: 23760265
  30. Resistance exercise resulted in a significant downregulation of MSTN and FBXO32 mRNA expression and a significant upregulation in FSTL3 and SMURF1 mRNA expression, and carbohydrate and protein feeding have little influence on the these markers expression. PMID: 22476926
  31. Smurf1 turnover is mediated by Ckip-1 and Rpt6. PMID: 23032291
  32. The expression of SMURF1 is enhanced in hepatocellular carcinoma, which may have played a role in the disease through affecting apoptosis and proliferation of hepatic cancer cells. PMID: 23042388
  33. USP9X is an important regulatory protein of SMURF1. PMID: 23184937
  34. Ndfip1 negatively regulates RIG-I-dependent immune signaling by enhancing E3 ligase Smurf1-mediated MAVS degradation. PMID: 23087404
  35. A Cdh1-APC/Smurf1/RhoA pathway that mediates axonal growth suppression in the developing mammalian brain. PMID: 22949615
  36. Smurf1 is a negative feedback regulator for IFN-gamma signaling by targeting STAT1 for ubiquitination and proteasomal degradation. PMID: 22474288
  37. Inflammatory cytokines led to direct activation of Smurf1 and downregulation of miR-17, thereby increasing degradation of Smurf1-mediated osteoblast-specific factors. PMID: 21898695
  38. studies uncover a cell-cycle-independent function of Cdh1, establishing Cdh1 as an upstream component that governs Smurf1 activity PMID: 22152476
  39. SMURF1 is an amplified oncogene driving multiple tumorigenic phenotypes in pancreatic cancer PMID: 21887346
  40. Data report that the C2 domain of Smurf1 is necessary and sufficient for binding RhoA, and therefore is crucial for targeting RhoA for ubiquitination. PMID: 21708152
  41. ER stress induces Smurf1 degradation and WFS1 up-regulation PMID: 21454619
  42. the Smurf1 C2 domain has a role in substrate selection and cellular localization PMID: 21402695
  43. This study provided the first evidence that Smurf1 functions as an E3 ligase to promote the ubiquitination and proteasomal degradation of KLF2. PMID: 21382345
  44. These results suggest a new role of Smurf1 in inflammation and immunity through controlling the degradation of TRAFs. PMID: 19937093
  45. plasma membrane localization of Smad7 by Smurf1 requires the C2 domain of Smurf1 and is essential for the inhibitory effect of Smad7 in the transforming growth factor-beta signaling pathway PMID: 12151385
  46. CRM1-dependent nuclear export of Smurf1 is essential for the negative regulation of TGF-beta signaling by Smad7. PMID: 12519765
  47. Smurf1 links the Cdc42/Rac1-PAR6 polarity complex to degradation of RhoA in lamellipodia and filopodia to prevent RhoA signaling during dynamic membrane movements PMID: 14657501
  48. Smad7 and Smurf1 have roles in regulation of TGF-beta signaling in scleroderma fibroblasts PMID: 14722617
  49. rhoA GTP-Binding Protein is targeted for ubiquitination and degradation via Smurf1 PMID: 15710384
  50. Smad6 interacts with Runx2 and mediates Smad ubiquitin regulatory factor 1-induced Runx2 degradation PMID: 16299379

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Subcellular Location
Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side.
Tissue Specificity
Expressed in melanocytes.
Database Links

HGNC: 16807

OMIM: 605568

KEGG: hsa:57154

STRING: 9606.ENSP00000354621

UniGene: Hs.189329

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