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CUSABIO meticulously developed the SSTR2 recombinant monoclonal antibody through a carefully executed procedure. Initially, B cells were isolated from the spleen of an animal immunized with the recombinant human SSTR2 protein. Subsequently, RNA was extracted from the B cells and converted into cDNA through reverse transcription. Using the cDNA as a template, the gene encoding the SSTR2 antibody was amplified with a degenerate primer and inserted into a vector. The recombinant vector was then transfected into host cells, facilitating the production of the SSTR2 recombinant monoclonal antibodies. Following expression, these antibodies were harvested from the cell culture supernatant and purified using affinity chromatography. This antibody can detect human SSTR2 protein in ELISA and FC applications.
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