Product Details

Uniprot No.

Target Names

Alternative Names

Homo sapiens interferon alpha induced transcriptional activator antibody; interferon alpha induced transcriptional activator antibody; ISGF 3 antibody; ISGF3 antibody; MGC59816 antibody; P113 antibody; signal transducer and activator of transcription 2 113kD antibody; Signal transducer and activator of transcription 2 antibody; STAT113 antibody; Stat2 antibody; STAT2_HUMAN antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human STAT2 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

4B3

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

STAT2 serves as a critical component of the interferon-stimulated gene factor 3 complex, functioning as an essential mediator of type I interferon signaling. When cells encounter viral infection or other immune challenges, STAT2 becomes phosphorylated and partners with STAT1 and IRF9 to drive the transcription of interferon-stimulated genes. This central role in antiviral defense and immune regulation makes STAT2 a valuable target for researchers investigating innate immunity, viral pathogenesis, and interferon-related disorders.

This recombinant monoclonal antibody, clone 4B3, offers the reproducibility that demanding signal transduction studies require. Because the antibody sequence is defined and produced recombinantly in rabbit host cells, you can expect consistent performance across experiments and between lot numbers, eliminating the variability that can complicate long-term research projects or multi-site collaborations.

Validation across multiple detection platforms demonstrates the flexibility of this reagent in your experimental workflows. Immunohistochemistry studies in paraffin-embedded human tonsil tissue using a Leica Bond system with citrate buffer antigen retrieval show clear target detection at 1:100 dilution. Immunofluorescence analysis in HeLa cells reveals distinct staining patterns when counterstained with DAPI, while flow cytometry experiments in 786-O renal carcinoma cells demonstrate a clear positive shift compared to isotype control. These validated applications at dilutions ranging from 1:50 to 1:200 provide starting points you can optimize for your specific experimental conditions.

Whether you are characterizing interferon responses in tissue sections, examining STAT2 localization in cultured cells, or quantifying expression levels by flow cytometry, this antibody supports diverse approaches to understanding signal transduction pathways in human samples.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

Signal transducer and activator of transcription that mediates signaling by type I interferons (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In addition, has also a negative feedback regulatory role in the type I interferon signaling by recruiting USP18 to the type I IFN receptor subunit IFNAR2 thereby mitigating the response to type I IFNs. Acts as a regulator of mitochondrial fission by modulating the phosphorylation of DNM1L at 'Ser-616' and 'Ser-637' which activate and inactivate the GTPase activity of DNM1L respectively.

Gene References into Functions

Priming cells with IFNbeta synergistically enhances IL6 induction in response to treatments that activate NF-kappaB, in a process that depends upon the recruitment of STAT2, IRF9. PMID: 29581268
Surface features in the interacting domains of IRF9 and STAT2 have diverged to enable specific interaction between these family members and to enable the antiviral response. PMID: 29317535
mRNA expression of survivin was positively correlated with STAT2 mRNA in psoriasis vulgaris lesion tissues PMID: 29089085
Based on the proposition that NS5 utilizes SIAH2-mediated proteasomal degradation of STAT2, an in-silico study was carried out to characterize the protein-protein interactions between NS5, SIAH2 and STAT2 proteins. PMID: 28365387
The pathways related to tumorigenicity and tumor progression, STAT2 and AdipoR1/AMPK/SIRT1 could be restrained by miR-3908. In conclusion, restoration of miR-3908 expression induced suppression of cancer progression and glioblastoma tumorigenicity. PMID: 28440504
highlight the existence of a STAT1-independent IFN-I signaling pathway, where STAT2/IRF9 can potentially substitute for the role of ISGF3 and offer a back-up response against viral infection. PMID: 27053489
Decreased phosphorylated STAT2 expression was accompanied by increased replication of hepatitis C virus and hepatitis E virus. PMID: 28442624
data suggest that STAT2 plays a role in the psoriasis pathogenesis by regulating the expression of CXCL11 and CCL5, and thereby attracting IFNgamma-producing immune cells to the skin PMID: 28472186
Interferon-alpha-enhanced IL-10 expression in human CD4 T cells is regulated by STAT3, STAT2, and BATF transcription factors. PMID: 28242623
the rate-limiting transition state for binding between the TAZ1 domain of CREB binding protein and the intrinsically disordered transactivation domain of STAT2 (TAD-STAT2) by site-directed mutagenesis and kinetic experiments (Phi-value analysis) and found that the native protein-protein binding interface is not formed at the transition state for binding. PMID: 28707474
these studies identify phosphorylation of S734-STAT2 as a new regulatory mechanism that negatively controls the type I IFN-antiviral response. PMID: 27802159
study demonstrates that overexpression of Porcine deltacoronavirus (PDCoV) nsp5 also antagonizes IFN signaling by cleaving STAT2, an essential component of transcription factor complex ISGF3, and that PDCoV infection reduces the levels of STAT2, which may affect the innate immune response PMID: 28250121
PKV VP3 associated with STAT2 and IRF9, and interfered with the formation of the STAT2-IRF9 and STAT2-STAT2 complex. PMID: 28441586
The authors have discovered a novel phosphorylation of STAT2 on T387 that negatively regulates signal from the type I interferon receptor to the genome by transcription factor ISGF3. PMID: 27852626
STAT2 recruits USP18 to the type I IFN receptor subunit IFNAR2 via its constitutive membrane-distal STAT2-binding site. PMID: 28165510
These findings indicate that overexpression of IFITM1 enhances the aggressive phenotype of triple-negative SUM149 IBC cells and that this effect is dependent on STAT2/BRG1 interaction. PMID: 26897526
IFN-beta exhibited significant cytotoxicity in HepG2 cells mainly through phosphorylation of STAT2. PMID: 25773664
STAT2 rs2066807 polymorphism is not associated with cervical cancer. PMID: 25921106
Respiratory syncytial virus NS1 upregulates SOCS1 expression in a RIG-I- and TLR3-independent pathway, to inhibit STAT2 phosphorylation. PMID: 24480984
Data indicate that the RVB1/2 chromatin-remodeling complex is required for efficient Pol II recruitment and initiation at IFN-alpha-stimulated genes (ISGs) promoters and is recruited through interaction with the STAT2 transactivation domain. PMID: 23878400
Dengue virus co-opts UBR4 to degrade STAT2 and antagonize type I interferon signaling. PMID: 23555265
Sequencing of genomic DNA and RNA revealed a homozygous mutation in intron 4 of STAT2 that prevented correct splicing in patient cells PMID: 23391734
In systemic lupus erythematosus but not in healthy control leukocytes, Jak1 and STAT2 were constitutively phosphorylated, even in the absence of disease activity. PMID: 22859983
STAT2 serine 287 is a novel regulatory phosphorylation site in type I interferon-induced cellular responses PMID: 23139419
These results indicate the importance that herpes simplex virus 2 has assigned to STAT2, investing significant genomic currency throughout its replicative lifecycle for continuous targeted destruction and inhibition of this protein. PMID: 22634037
A haplotype at STAT2 introgressed from neanderthals serves as a candidate of positive selection in Papua New Guinea. PMID: 22883142
STAT2 is functionally involved in the immunosuppressive activity of hcMSCs as a novel regulator under inflammatory conditions. PMID: 22523757
Co-immunoprecipitation/immunoblot analyses showed that STAT2 enhanced RCAN1 ubiquitination through the ubiquitin E3 ligase FBW7.. PMID: 22426484
Data show that, in response to IFNalpha, STAT2 is recruited onto the endogenous P2p73 promoter together with the polycomb group protein Ezh2. PMID: 21399658
Chromatin dynamics of gene activation and repression in response to interferon alpha (IFN(alpha)) reveal new roles for phosphorylated and unphosphorylated forms of the transcription factor STAT2. PMID: 21498520
STAT2 may interact with IRF-9 in a STAT1-independent manner. The complex STAT2/IRF-9 is the key factor mediating the expression of RIG-G gene regulated by IFN-alpha. PMID: 20403236
Signals ensued by IFN-alpha and IL-4 induce cytoplasmic sequestration of IL-4-activated STAT6 and IFN-alpha-activated STAT2:p48 in B cells through the formation of pY-STAT6:pY-STAT2:p48 complex. PMID: 21268015
Dengue virus 2 NS5 is able to bind and degrade human STAT2. PMID: 21075352
STAT2 and IRF-1 comppete at binding interferon-stimulated response elements (ISREs) located on the retinoic acid-induced gene G (RIG-G) promoter in RIG-G. PMID: 20533260
Defects in the expression or nuclear localization of STAT2 could lessen the efficacy of type I IFN immunotherapy. PMID: 20068068
In this study we have characterized the Stat2-IFNaR2 interaction and examined its role in IFNalpha signaling PMID: 11786546
IFNtau did not affect IRF-1 expression in Stat2-deficient cells PMID: 11804954
acts as a host range determinant for species-specific paramyxovirus interferon antagonism and simian virus 5 replication PMID: 12050355
Stat2 binds more avidly to subunit 2 of the interferon alpha receptor (IRNaR2) than to phosphorylated IFNaR1. PMID: 12220192
Nipah virus V protein evades alpha and gamma interferons by preventing STAT2 activation and nuclear accumulation PMID: 12388709
results suggest that the STAT2 activation process is a crucial target for the blockade of IFN-alpha signaling PMID: 12610111
Measles virus V protein blocks INF-alpha/beta by inhibiting STAT2 phosphorylation. PMID: 12804771
Hendra virus V protein inhibits cellular responses to IFN through binding and cytoplasmic sequestration of STAT2. PMID: 14557668
there is a critical motif in Stat2 required for its transcriptional activity, and resistance to type one IFNs can be mediated by mutations in Stat2 PMID: 14722125
expression of STAT-2 induced by Epstein-Barr virus (EBV) LMP-1 (latent membrane protein) and may be part of the viral programming that regulates viral latency and cellular transformation PMID: 15165826
The regulation of STAT2 nuclear trafficking is distinct from the previously characterized STAT1 factor. PMID: 15175343
IFNalpha-induced nuclear accumulation of STAT2 was partially blocked in cell lines expressing high levels of HCV core protein. Subsequently PMID: 15221897
STAT2 activation and interferon signaling blocked by West Nile Virus nonstructural proteins PMID: 15650219
the conserved DNA-binding domain of STAT2 has a role specific to the activity of ISGF3-independent STAT2-containing complexes PMID: 15668228
IFNaR2 intracellular domain transcriptional modulation is dependent upon the carboxyl-terminal transactivation domain of Stat2. PMID: 15717316

Hide All

Involvement in disease

Immunodeficiency 44 (IMD44)

Subcellular Location

Cytoplasm. Nucleus.

Protein Families

Transcription factor STAT family

Database Links

HGNC: 11363

OMIM: 600556

KEGG: hsa:6773

STRING: 9606.ENSP00000315768

UniGene: Hs.530595

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Code	CSB-RA944215A0HU
Size	US$210
	Order now
Image	IHC image of CSB-RA944215A0HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of Hela cell with CSB-RA944215A0HU at 1:50 , counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing 786-O cells stained with CSB-RA944215A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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STAT2 Recombinant Monoclonal Antibody

Product Details

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Target Background