Code | CSB-RA843206A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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WB | 1:500-1:5000 |
B cells were inducibly generated by a synthesized peptide obtained from human TMEM173, and subsequently fused with myeloma cells to create hybridomas. The variable light (VL) and variable heavy (VH) domains of TMEM173 antibody-producing hybridomas' cDNA were sequenced and used as a model for recombinant vector construction. The next step is to transfect the TMEM173 monoclonal antibody gene-containing vector into cells for culturing. The TMEM173 recombinant monoclonal antibody was then obtained and purified from the cell culture supernatant through affinity chromatography. The purified antibody was tested for specificity in ELISA and WB applications and was found to detect only human TMEM173 protein.
The TMEM173 protein, also known as STING, plays an important role in the innate immune system by activating the production of interferons and other cytokines in response to the detection of microbial DNA in the cytosol of infected cells. The TMEM173 pathway is important for the detection of viral infections and the clearance of intracellular bacteria, and dysregulation of this pathway has been implicated in various autoimmune and inflammatory diseases.
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