TUBB1 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA867148MA1HU
Size	US$210
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Image	Western Blot Positive WB detected in: K562 whole cell lysate All lanes: TUBB1 antibody at 1:1000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 50 kDa Observed band size: 50 kDa IHC image of CSB-RA867148MA1HU diluted at 1:300 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA867148MA1HU diluted at 1:300 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing NIH/3T3 cells stained with CSB-RA867148MA1HU (red line) at 1:200. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details
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Target Background

Product Details

Uniprot No.

Q9H4B7

Target Names

TUBB1

Alternative Names

2810484G07Rik antibody; beta 1 tubulin antibody; Beta tubulin 1, class VI antibody; Beta-tubulin antibody; Class VI beta tubulin antibody; dJ543J19.4 antibody; M(beta)1 antibody; TBB1_HUMAN antibody; TUBB1 antibody; Tubulin beta 1 class VI antibody; Tubulin beta-1 chain antibody; Tubulin, beta 1 antibody; tubulin, beta1 antibody

Species Reactivity

Human

Immunogen

Recombinant Human TUBB1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Mouse IgG

Clone No.

20B1

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:20-1:200
FC	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

To create the TUBB1 recombinant monoclonal antibody, the process begins with the acquisition of the TUBB1 antibody genes. These genes are then introduced into suitable host cells, where they serve as the basis for synthesizing TUBB1 antibodies utilizing a cell-based expression and translation system. This method offers several advantages, including significantly enhancing the purity and stability of the resulting TUBB1 recombinant monoclonal antibodies, as well as boosting their affinity and specificity. Following synthesis, the TUBB1 recombinant monoclonal antibody undergoes a purification step utilizing affinity chromatography. Subsequently, it undergoes thorough testing through various assays, including ELISA, IHC, and FC. This antibody exclusively recognizes the human TUBB1 protein.

TUBB1 is a critical component of microtubules, and its primary role is to contribute to the structural integrity of the cytoskeleton and participate in various cellular processes, including intracellular transport, cell division, cell motility, and intracellular organization. Dysfunction in microtubule dynamics can have significant implications for cell function and may contribute to diseases such as cancer and neurological disorders.

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Target Background

Function

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Gene References into Functions

Neonatal platelets exhibit low levels of the Stx11-Munc18b complex (essential component of the SNARE machinery) and of beta1-tubulin. These developmental deficiencies are associated with defects in platelet adhesion, spreading and secretion. PMID: 29044293
novel DCX mutation (p.D90G, NP_000546.2) appeared to be a major causative variant, whereas the novel mutation of TUBB1 (p.R62fsX, NP_110400.1) was found only in patients with more-severe intellectual disability in Familial pachygyria PMID: 26743950
Analysis of the TUBB1 gene revealed three known missense variants in heterozygous state which in combination might explain the beta1-tubulin defect. PMID: 26540125
TUBB1 R307H SNP is significantly associated with the degree of thrombocytopenia in congenital and acquired platelet disorders, and may affect platelets by altering microtubule behavior. PMID: 25529050
Data indicate that ABCB1 protein, beta tubulin I and III (betaI, and betaIII tubulin) might contribute to the multidrug resistance (MDR) of MCF7/DOC and be potential therapeutic targets for overcoming MDR of breast cancer. PMID: 24894670
TUBB1 mutation disrupting microtubule assembly impairs proplatelet formation and results in congenital macrothrombocytopenia. PMID: 24344610
our findings define beta-tubulin VI as a hematologic isotype with significant genetic variation in humans that may affect the myelosuppresive action of microtubule-binding drugs PMID: 22805305
homozygous status of P43 genetic polymorphism causes alterations in platelet ultrastructure PMID: 21384078
Studies show that BFBTS bound and modified beta-tubulin at residue Cys12, forming beta-tubulin-SS-fluorobenzyl. PMID: 19996274
SLPI localizes in part along the megakaaryocyte and platelet cytoskeleton by virtue of specific interactions with beta1 tubulin. PMID: 15315966
the platelet Q43P beta1-tubulin substitution is frequent in the healthy population and may protect men against arterial thrombosis PMID: 15956286
The TUBB1 Q43P polymorphism, by causing a lower reactivity in platelets carrying the variant form of b1-tubulin, protects against thrombotic disorders but increases the risk of intracerebral hemorrhage in men. PMID: 17488662
biophysical analysis of carboxy-terminal tail conformation of human beta-tubulin isotypes PMID: 17993481
Mutation of the beta1-tubulin gene associated with congenital macrothrombocytopenia affecting microtubule assembly. PMID: 18849486
TUBB1 Q43P polymorphism does not protect against acute coronary syndrome and premature myocardial infarction. PMID: 19132255

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Involvement in disease

Macrothrombocytopenia, autosomal dominant, TUBB1-related (MAD-TUBB1)

Subcellular Location

Cytoplasm, cytoskeleton.

Protein Families

Tubulin family

Tissue Specificity

Hematopoietic cell-specific. Major isotype in leukocytes, where it represents 50% of all beta-tubulins.

Database Links

HGNC: 16257

OMIM: 612901

KEGG: hsa:81027

STRING: 9606.ENSP00000217133

UniGene: Hs.303023

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Protein Service

Gene Synthesis Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Gap junction Target Therapy

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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