Product Details

Purity

≥ 95% as determined by SEC-HPLC.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Human CD37 at 10 μg/mL can bind Anti-CD37 recombinant antibody(CSB-RA004928MA1HU). The EC50 is 7.458-9.930 ng/mL.The VLPs (CSB-MP3838) is negative control.

Target Names

CD37

Uniprot No.

P11049

Alternative Names

Leukocyte antigen CD37; Tetraspanin-26 (Tspan-26); CD37; TSPAN26

Species

Homo sapiens (Human)

Source

Mammalian cell

Expression Region

1-281aa

Target Protein Sequence

MSAQESCLSLIKYFLFVFNLFFFVLGSLIFCFGIWILIDKTSFVSFVGLAFVPLQIWSKVLAISGIFTMGIALLGCVGALKELRCLLGLYFGMLLLLFATQITLGILISTQRAQLERSLRDVVEKTIQKYGTNPEETAAEESWDYVQFQLRCCGWHYPQDWFQVLILRGNGSEAHRVPCSCYNLSATNDSTILDKVILPQLSRLGHLARSRHSADICAVPAESHIYREGCAQGLQKWLHNNLISIVGICLGVGLLELGFMTLSIFLCRNLDHVYNRLARYR

Mol. Weight

33.1 kDa

Protein Length

Full Length

Tag Info

C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions)

Form

Lyophilized powder
Note: We will default ship it in lyophilized form with normal bule ice packs. However, if you request to ship in liquid form, it needs to be shipped with dry ice, please communicate with us in advance and extra fees for dry ice and dry ice box will be charged.

Buffer

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

The VLPs are expressed from human 293 cells (HEK293).Mix the sample gently by repeatedly pipetting it up and down. Do not vortex.Repeated freezing and thawing is not recommended.Store the protein at -20°C/-80°C upon receiving it, and ensure to avoid repeated freezing and thawing, otherwise, it will affect the protein activity. The immunization strategy should be optimized (antigen dose, regimen and adjuvant).

Datasheet & COA

Please contact us to get it.

Description

CD37, a tetraspanin enriched on B cells and implicated in lymphoma pathogenesis, forms multimeric complexes that regulate immune cell signaling and migration, making native quaternary structure essential for functional studies. This full-length protein (aa 1–281) is presented as virus-like particles that preserve the native membrane topology and tetramer assembly characteristic of tetraspanins, enabling physiologically relevant binding interactions. Functional ELISA validation demonstrates specific antibody recognition with an EC50 of 7.458–9.930 ng/mL against an anti-CD37 recombinant antibody, confirming proper epitope presentation and supporting use in antibody development campaigns, immune cell trafficking studies, and tumor metastasis models where CD37-mediated cell–cell interactions drive disease progression. Endotoxin levels below 1.0 EU/μg align with standards expected in cell-based migration and invasion assays where inflammatory contamination would confound readouts of CD37's role in B-cell malignancies.

Target Background

Gene References into Functions

CD37 is a critical determinant of rituximab plus cyclophosphamide, doxorubicin, vincristine, and prednisone outcome in diffuse large B-cell lymphoma. PMID: 27760757
Data indicate that cell differentiation antigen 37 (CD37) is well expressed and a potential drug target in acute myeloid leukemia (AML). PMID: 25934707
The CD37-targeted antibody-drug conjugate IMGN529 is highly active against human CLL and in a novel CD37 transgenic murine leukemia model. PMID: 24445867
Data indicate that enhanced antibody-dependent cell cytotoxicity (ADCC) is observed against chronic lymphocytic leukemia cells and is sustained at concentrations of SMIP-016(GV) as low at 5E(-6) microg/mL on cells expressing minimal CD37 antigen. PMID: 23883821
Identify two tyrosine residues in tetraspanin CD37 directly mediating transduction of survival and apoptotic signals. PMID: 22624718
In the absence of both CD37 and Tssc6, immune function is further altered when compared with CD37- or Tssc6-deficient transgenic mice, demonstrating a complementary role for these two molecules in cellular immunity. PMID: 20709950
CD37 cross-linking on human T cells transduces signals that lead to complete inhibition of CD3-induced T cell proliferation. PMID: 14978098
CD37 is important for dectin-1 stabilization in APC membranes and controls dectin-1-mediated IL-6 production. PMID: 17182550
provide strong justification for CD37 as a therapeutic target and introduce small modular immunopharmaceuticals as a novel class of targeted therapies for B-cell malignancies PMID: 17440052

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Subcellular Location

Membrane; Multi-pass membrane protein.

Protein Families

Tetraspanin (TM4SF) family

Tissue Specificity

B-lymphocytes.

Database Links

HGNC: 1666

OMIM: 151523

KEGG: hsa:951

STRING: 9606.ENSP00000325708

UniGene: Hs.166556

Code	CSB-MP004928HU
Abbreviation	Recombinant Human CD37 protein-VLPs (Active)
MSDS	MSDS Datasheet
Size	$558
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Image	CSB-MP004928HU is detected by Mouse anti-10*His monoclonal antibody. Activity Measured by its binding ability in a functional ELISA. Immobilized Human CD37 at 10 μg/ml can bind Anti-CD37 recombinant antibody(CSB-RA004928MA1HU). The EC₅₀ is 7.458-9.930 ng/mL.The VLPs (CSB-MP3838) is negative control. Biological Activity Assay The purity of VLPs was greater than 95% as determined by SEC-HPLC
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Recombinant Human Leukocyte antigen CD37 (CD37)-VLPs (Active)

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