Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

TXNIP

Uniprot No.

Q9H3M7

Research Area

others

Alternative Names

TXNIP; VDUP1; Thioredoxin-interacting protein; Thioredoxin-binding protein 2; Vitamin D3 up-regulated protein 1

Species

Homo sapiens (Human)

Source

in vitro E.coli expression system

Expression Region

1-391aa

Target Protein Sequence

MVMFKKIKSFEVVFNDPEKVYGSGEKVAGRVIVEVCEVTRVKAVRILACGVAKVLWMQGSQQCKQTSEYLRYEDTLLLEDQPTGENEMVIMRPGNKYEYKFGFELPQGPLGTSFKGKYGCVDYWVKAFLDRPSQPTQETKKNFEVVDLVDVNTPDLMAPVSAKKEKKVSCMFIPDGRVSVSARIDRKGFCEGDEISIHADFENTCSRIVVPKAAIVARHTYLANGQTKVLTQKLSSVRGNHIISGTCASWRGKSLRVQKIRPSILGCNILRVEYSLLIYVSVPGSKKVILDLPLVIGSRSGLSSRTSSMASRTSSEMSWVDLNIPDTPEAPPCYMDVIPEDHRLESPTTPLLDDMDGSQDSPIFMYAPEFKFMPPPTYTEVDPCILNNNVQ
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

57.7kDa

Protein Length

Full Length

Tag Info

N-terminal 6xHis-B2M-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The expression region of this recombinant Human TXNIP covers amino acids 1-391. The expected molecular weight for the TXNIP protein is calculated to be 57.7 kDa. This TXNIP recombinant protein is manufactured in in vitro e.coli expression system. The N-terminal 6xHis-B2M tag was fused into the coding gene segment of TXNIP, making it easier to detect and purify the TXNIP recombinant protein in the later stages of expression and purification.

Thioredoxin-interacting protein (TXNIP) is a protein primarily associated with cellular oxidative stress, glucose metabolism, and disease development. In the field of oxidative stress, TXNIP regulates the redox balance within cells by interacting with thioredoxin, playing a crucial role in maintaining cellular homeostasis. Regarding glucose metabolism, TXNIP is a key regulatory factor in glucose metabolism, participating in insulin secretion and the modulation of cellular sensitivity to glucose. Besides, the association of TXNIP with various diseases, including diabetes, cardiovascular diseases, and cancer, has garnered widespread attention.

Target Background

Function

May act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability. Interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm. Functions as a transcriptional repressor, possibly by acting as a bridge molecule between transcription factors and corepressor complexes, and over-expression will induce G0/G1 cell cycle arrest. Required for the maturation of natural killer cells. Acts as a suppressor of tumor cell growth. Inhibits the proteasomal degradation of DDIT4, and thereby contributes to the inhibition of the mammalian target of rapamycin complex 1 (mTORC1).

Gene References into Functions

Using oxygen-glucose deprivation and reoxygenation (OGD/R) to create a cell model of hepatic I/R injury, we found that the mRNA and protein expression levels of TXNIP were upregulated in HL7702cells exposed to OGD/R. PMID: 29852169
Expression of TXNIP2 isoform, not TXNIP1, is upregulated in leukocytes of patients with acute myocardial infarction. PMID: 30034557
Thioredoxin-interacting protein (TXNIP) is highly induced in retinal vascular endothelial cells under diabetic conditions. Data (including data from studies using knockout mice) suggest that TXNIP in retinal vascular endothelial cells plays role in diabetic retinal angiogenesis via VEGF/VEGFR2 and Akt/mTOR signaling. (VEGFR2 = vascular endothelial growth factor receptor-2) PMID: 29203232
miR-20a could negatively regulate TLR4 and NLRP3 signaling to protect human aortic endothelial cells from inflammatory injuries. PMID: 29653364
Results found the mRNA level of TRX-1 was significantly decreased (p<0.005), while the mRNA levels of TBP-2, COX-2, and TNF-alpha were significantly increased in the placentas in preeclampsia when compared to the normal group. PMID: 29999276
Consistent with its enhanced expression in Laron syndrome, we provide evidence that TXNIP gene expression is negatively regulated by IGF1. PMID: 29339473
TXNIP contributes to the dysregulation of tubular autophagy and mitophagy in diabetic nephropathy through activation of the mTOR signaling pathway. PMID: 27381856
This study thus characterizes ERK-mediated suppression of TXNIP as a presently unreported mechanism by which ap junctions regulate cell behaviors. PMID: 28694028
Results indicate an internal ribosome entry sites (IRESes) within the thioredoxin-interacting protein (TXNIP) protein; 5' untranslated region (5'UTR), and regulatory IRES trans-acting factors. PMID: 29378331
The data of this study suggested that TXNIP blocked autophagic flux and induced alpha-synuclein accumulation through inhibition of ATP13A2. PMID: 28755477
Compared with normal tissues, TXNIP expression was significantly decreased in human breast cancer tissues and canine mammary tumors, along with tumor progression. PMID: 29524408
This finding suggested statistically significant role of interaction of TXNIP and AF1q polymorphisms (TXNIP-rs2236566, TXNIP-rs7211, and AF1q-rs2769605) in schizophrenia susceptibility. PMID: 27510506
summarize the current state of our understanding of TXNIP biology, highlight its role in metabolic regulation and raise critical questions that could help future research to exploit TXNIP as a therapeutic target PMID: 28137209
intracellular TXNIP protein is a critical regulator of activation of the fructose-induced NLRP3 inflammasome PMID: 28326454
This study provides insight into the molecular mechanisms of TXNIP overexpression in liver cancer cell survival and apoptosis and indicated that TXNIP may be a novel promising agent for liver cancer treatment. PMID: 28440491
Study showed that the expression of TXNIP was significantly increased in RNF2 knockdown prostate tumor cells and that TXNIP is an important downstream target of RNF2. PMID: 28029659
Depletion of glycolytic intermediates led to a consistent decrease in TXNIP expression in response to 1,25(OH)2D3, an effect that coincided with the activation of AMPK signaling and a reduction in c-MYC expression. PMID: 28651973
We found no evidence of decreased TXNIP DNA methylation or increased gene expression in metabolic target tissues of offspring exposed to maternal diabetes. Further studies are needed to confirm and understand the paradoxical SAT TXNIP DNA methylation and gene expression changes in O-GDM subjects PMID: 29077742
the association of TXNIP (thioredoxin-interacting protein) with NLRP3 induced by ROS promoted NLRP3 inflammasome activation in senescent HUVEC endothelial cells PMID: 28064010
histone acetylation serves as a key regulator of glucose-induced increase in TXNIP gene expression PMID: 27989964
thioredoxin-interacting protein deficiency alleviates diabetic renal lipid accumulation through regulation of Akt/mTOR pathway PMID: 27497988
High expression of TXNIP indicates a lower pathological grade of meningnioma, and is also associated with longer recurrence-free time. PMID: 28243945
All-trans retinoic acid plays a key role in inhibition of hepatic stellate cell activation via suppressing TXNIP expression, which reduces oxidative stress levels. PMID: 28322443
Study shows that TXNIP expression is down-regulated in new Multiple Sclerosis (MS) patients compared to controls and might be implicated in pathogenesis of the disease. PMID: 28844667
TXNIP Single nucleotide polymorphisms may individually and cumulatively affect CAD risk through a possible mechanism for regulating TXNIP expression and gene-environment interactions. PMID: 27470124
Taken together, our results firstly reveal that TMAO induces inflammation and endothelial dysfunction via activating ROS-TXNIP-NLRP3 inflammasome, suggest a likely mechanism for TMAO-dependent enhancement in atherosclerosis and cardiovascular risks. PMID: 27833015
results suggest that TXNIP is required early in the apoptotic-inducing pathway resulting from r-Moj-DM binding to the alphav integrin subunit PMID: 27567705
Hypoxia suppresses thioredoxin binding protein-2 gene expression, which may ultimately alter placental development. PMID: 27762463
Heme oxygenase rs2071749 polymorphism was positively associated with obesity in Mexicans. PMID: 27274779
These data identify novel Txnip protein interactions. PMID: 27437069
Glucose exerts strong stimulatory effects on activation histone marks while having inhibitory effects on repression marks in the promoter of the TXNIP gene, and this was associated with a marked increase in expression of the proinflammatory gene in kidney. PMID: 26806835
No evidence that SNPS in TXNIP has effect, but the rs4485648 polymorphism of the TrxR2 gene might exert an independent effect on the development of Diabetic retinopathy. PMID: 26763822
An association has been found between the TXNIP gene methylation pattern and type 2 diabetes mellitus in ischemic stroke patients through epigenetic mechanisms, related to sustained hyperglycemia levels with HbA1c >/= 7%. PMID: 26643952
These findings thereby provide new mechanistic insight into the regulation of TXNIP and beta-cell biology and reveal novel links between proinflammatory cytokines, carbohydrate response element binding protein-mediated transcription, and microRNA signaling. PMID: 26858253
The crystal structure of the complex between a phosphorylated PPxY motif of TXNIP and the SH2 domain of Vav2 reveals a conserved recognition mechanism. PMID: 26919541
Activation of the miR-373-TXNIP-HIF1alpha-TWIST signaling axis is correlated with a worse outcome in patients with breast cancer. PMID: 26196741
Metformin down-regulates high-glucose-induced TXNIP transcription by inactivating ChREBP and FOXO1 in endothelial cells, partially through AMP-activated protein kinase activation PMID: 26147751
Foam cell-released 4-hydroxnonenal activates PPARdelta in Vascular endothelial cells, leading to increased TXNIP expression and consequently to senescence. PMID: 25754218
our data support the hypothesis that TXNIP is an effective target for the treatment of breast cancer. PMID: 25812606
Expression of TXNIP was up-regulated in all three NSCLC cell lines. PMID: 25854388
Data identify the metastasis suppressor TXNIP as new target of miR-224/miR-452 that induces feedback inhibition of E2F1 and show that miR-224/452-mediated downregulation of TXNIP is essential for E2F1-induced EMT and invasion PMID: 25341426
HG-induced NADPH oxidase activation is driven by TXNIP which subsequently triggers NALP3 inflammasome activation in podocytes and ultimately led to podocyte injury PMID: 25834832
Suggest that TXNIP plays a critical role in anti-Her-1/Her-2 treatment and may be a potential prognostic marker in breast cancer. PMID: 25605021
The expression of TXNIP was significantly higher in normal-weight type- 2 diabetic patients than in obese ones. PMID: 25726203
The inhibition of TRX by TXNIP is mediated by an intermolecular disulphide interaction resulting from a novel disulphide bond-switching mechanism. This bond dissociates in the presence of high concentrations of reactive oxygen species. PMID: 24389582
The protein expression level of TXNIP was negatively correlated with the level of miR-373 in MCF-7 cells and in breast cancer tissue of various migration and invasion abilities. TXNIP was regulated by miR-373. PMID: 26122224
data suggest that loss of the p53 tumor suppressor cooperates with Mychigh/TXNIPlow-driven metabolic dysregulation to drive the aggressive clinical behavior of TNBC. PMID: 25870263
diabetes was associated with a >30-fold increase in TxnIP gene expression and a 17 % increase in TxnIP protein expression. PMID: 24925443
TXNIP has been discovered to control pancreatic B-cell microRNA expression, B-cell function, and insulin production. [Review] PMID: 24911120
regulatory relationship between mTOR and the MondoA-TXNIP axis that we propose contributes to glucose homeostasis PMID: 25332233

Hide All

Subcellular Location

Cytoplasm.

Protein Families

Arrestin family

Database Links

HGNC: 16952

OMIM: 606599

KEGG: hsa:10628

STRING: 9606.ENSP00000358323

UniGene: Hs.533977

Code	CSB-CF880966HUa6
Abbreviation	Recombinant Human TXNIP protein
MSDS	MSDS Datasheet
Size	$878
	Order now
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Have Questions?	Leave a Message or Start an on-line Chat

Recombinant Thioredoxin-interacting protein (TXNIP)

Product Details

Related Products

Customer Reviews and Q&A

Target Background

×CloseMessage