Product Details

Uniprot No.

Q9H3M7

Target Names

TXNIP

Alternative Names

Thioredoxin-interacting protein (Thioredoxin-binding protein 2) (Vitamin D3 up-regulated protein 1), TXNIP, VDUP1

Species Reactivity

Human

Immunogen

A synthesized peptide from human TXNIP protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

5D2

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Thioredoxin-interacting protein serves as a critical regulator of cellular redox homeostasis and metabolic signaling, functioning as an endogenous inhibitor of thioredoxin. This multifaceted protein, also known as vitamin D3 up-regulated protein 1, has emerged as a key player in glucose metabolism, oxidative stress responses, and inflammasome activation, making it a compelling target for researchers investigating metabolic disorders, inflammatory diseases, and cancer biology.

This recombinant monoclonal antibody, generated from clone 5D2, offers the reproducibility and consistency that demanding experimental workflows require. Because recombinant antibodies are produced from defined sequences rather than hybridoma-derived sources, researchers benefit from lot-to-lot uniformity that supports reliable data across extended studies and multi-site collaborations.

Validation across multiple detection platforms demonstrates this antibody's versatility in your experimental toolkit. Western blot analysis in THP-1 and K562 cell lysates reveals a band at approximately 53 kDa, which runs higher than the predicted 44 kDa molecular weight—a shift commonly attributed to post-translational modifications such as glycosylation or phosphorylation that are characteristic of TXNIP in cellular contexts. Immunohistochemical staining has been validated in paraffin-embedded human cervical cancer tissue, providing clear visualization of TXNIP expression patterns in clinical specimens. Flow cytometry applications have been confirmed using K562 cells, enabling quantitative analysis of intracellular TXNIP levels at the single-cell level.

Whether investigating TXNIP's role in NLRP3 inflammasome activation, exploring its function as a tumor suppressor, or examining its involvement in diabetic complications, this antibody provides a dependable tool for advancing your understanding of redox-sensitive signaling pathways.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

May act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability. Interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm. Functions as a transcriptional repressor, possibly by acting as a bridge molecule between transcription factors and corepressor complexes, and over-expression will induce G0/G1 cell cycle arrest. Required for the maturation of natural killer cells. Acts as a suppressor of tumor cell growth. Inhibits the proteasomal degradation of DDIT4, and thereby contributes to the inhibition of the mammalian target of rapamycin complex 1 (mTORC1).

Gene References into Functions

Using oxygen-glucose deprivation and reoxygenation (OGD/R) to create a cell model of hepatic I/R injury, we found that the mRNA and protein expression levels of TXNIP were upregulated in HL7702cells exposed to OGD/R. PMID: 29852169
Expression of TXNIP2 isoform, not TXNIP1, is upregulated in leukocytes of patients with acute myocardial infarction. PMID: 30034557
Thioredoxin-interacting protein (TXNIP) is highly induced in retinal vascular endothelial cells under diabetic conditions. Data (including data from studies using knockout mice) suggest that TXNIP in retinal vascular endothelial cells plays role in diabetic retinal angiogenesis via VEGF/VEGFR2 and Akt/mTOR signaling. (VEGFR2 = vascular endothelial growth factor receptor-2) PMID: 29203232
miR-20a could negatively regulate TLR4 and NLRP3 signaling to protect human aortic endothelial cells from inflammatory injuries. PMID: 29653364
Results found the mRNA level of TRX-1 was significantly decreased (p<0.005), while the mRNA levels of TBP-2, COX-2, and TNF-alpha were significantly increased in the placentas in preeclampsia when compared to the normal group. PMID: 29999276
Consistent with its enhanced expression in Laron syndrome, we provide evidence that TXNIP gene expression is negatively regulated by IGF1. PMID: 29339473
TXNIP contributes to the dysregulation of tubular autophagy and mitophagy in diabetic nephropathy through activation of the mTOR signaling pathway. PMID: 27381856
This study thus characterizes ERK-mediated suppression of TXNIP as a presently unreported mechanism by which ap junctions regulate cell behaviors. PMID: 28694028
Results indicate an internal ribosome entry sites (IRESes) within the thioredoxin-interacting protein (TXNIP) protein; 5' untranslated region (5'UTR), and regulatory IRES trans-acting factors. PMID: 29378331
The data of this study suggested that TXNIP blocked autophagic flux and induced alpha-synuclein accumulation through inhibition of ATP13A2. PMID: 28755477
Compared with normal tissues, TXNIP expression was significantly decreased in human breast cancer tissues and canine mammary tumors, along with tumor progression. PMID: 29524408
This finding suggested statistically significant role of interaction of TXNIP and AF1q polymorphisms (TXNIP-rs2236566, TXNIP-rs7211, and AF1q-rs2769605) in schizophrenia susceptibility. PMID: 27510506
summarize the current state of our understanding of TXNIP biology, highlight its role in metabolic regulation and raise critical questions that could help future research to exploit TXNIP as a therapeutic target PMID: 28137209
intracellular TXNIP protein is a critical regulator of activation of the fructose-induced NLRP3 inflammasome PMID: 28326454
This study provides insight into the molecular mechanisms of TXNIP overexpression in liver cancer cell survival and apoptosis and indicated that TXNIP may be a novel promising agent for liver cancer treatment. PMID: 28440491
Study showed that the expression of TXNIP was significantly increased in RNF2 knockdown prostate tumor cells and that TXNIP is an important downstream target of RNF2. PMID: 28029659
Depletion of glycolytic intermediates led to a consistent decrease in TXNIP expression in response to 1,25(OH)2D3, an effect that coincided with the activation of AMPK signaling and a reduction in c-MYC expression. PMID: 28651973
We found no evidence of decreased TXNIP DNA methylation or increased gene expression in metabolic target tissues of offspring exposed to maternal diabetes. Further studies are needed to confirm and understand the paradoxical SAT TXNIP DNA methylation and gene expression changes in O-GDM subjects PMID: 29077742
the association of TXNIP (thioredoxin-interacting protein) with NLRP3 induced by ROS promoted NLRP3 inflammasome activation in senescent HUVEC endothelial cells PMID: 28064010
histone acetylation serves as a key regulator of glucose-induced increase in TXNIP gene expression PMID: 27989964
thioredoxin-interacting protein deficiency alleviates diabetic renal lipid accumulation through regulation of Akt/mTOR pathway PMID: 27497988
High expression of TXNIP indicates a lower pathological grade of meningnioma, and is also associated with longer recurrence-free time. PMID: 28243945
All-trans retinoic acid plays a key role in inhibition of hepatic stellate cell activation via suppressing TXNIP expression, which reduces oxidative stress levels. PMID: 28322443
Study shows that TXNIP expression is down-regulated in new Multiple Sclerosis (MS) patients compared to controls and might be implicated in pathogenesis of the disease. PMID: 28844667
TXNIP Single nucleotide polymorphisms may individually and cumulatively affect CAD risk through a possible mechanism for regulating TXNIP expression and gene-environment interactions. PMID: 27470124
Taken together, our results firstly reveal that TMAO induces inflammation and endothelial dysfunction via activating ROS-TXNIP-NLRP3 inflammasome, suggest a likely mechanism for TMAO-dependent enhancement in atherosclerosis and cardiovascular risks. PMID: 27833015
results suggest that TXNIP is required early in the apoptotic-inducing pathway resulting from r-Moj-DM binding to the alphav integrin subunit PMID: 27567705
Hypoxia suppresses thioredoxin binding protein-2 gene expression, which may ultimately alter placental development. PMID: 27762463
Heme oxygenase rs2071749 polymorphism was positively associated with obesity in Mexicans. PMID: 27274779
These data identify novel Txnip protein interactions. PMID: 27437069
Glucose exerts strong stimulatory effects on activation histone marks while having inhibitory effects on repression marks in the promoter of the TXNIP gene, and this was associated with a marked increase in expression of the proinflammatory gene in kidney. PMID: 26806835
No evidence that SNPS in TXNIP has effect, but the rs4485648 polymorphism of the TrxR2 gene might exert an independent effect on the development of Diabetic retinopathy. PMID: 26763822
An association has been found between the TXNIP gene methylation pattern and type 2 diabetes mellitus in ischemic stroke patients through epigenetic mechanisms, related to sustained hyperglycemia levels with HbA1c >/= 7%. PMID: 26643952
These findings thereby provide new mechanistic insight into the regulation of TXNIP and beta-cell biology and reveal novel links between proinflammatory cytokines, carbohydrate response element binding protein-mediated transcription, and microRNA signaling. PMID: 26858253
The crystal structure of the complex between a phosphorylated PPxY motif of TXNIP and the SH2 domain of Vav2 reveals a conserved recognition mechanism. PMID: 26919541
Activation of the miR-373-TXNIP-HIF1alpha-TWIST signaling axis is correlated with a worse outcome in patients with breast cancer. PMID: 26196741
Metformin down-regulates high-glucose-induced TXNIP transcription by inactivating ChREBP and FOXO1 in endothelial cells, partially through AMP-activated protein kinase activation PMID: 26147751
Foam cell-released 4-hydroxnonenal activates PPARdelta in Vascular endothelial cells, leading to increased TXNIP expression and consequently to senescence. PMID: 25754218
our data support the hypothesis that TXNIP is an effective target for the treatment of breast cancer. PMID: 25812606
Expression of TXNIP was up-regulated in all three NSCLC cell lines. PMID: 25854388
Data identify the metastasis suppressor TXNIP as new target of miR-224/miR-452 that induces feedback inhibition of E2F1 and show that miR-224/452-mediated downregulation of TXNIP is essential for E2F1-induced EMT and invasion PMID: 25341426
HG-induced NADPH oxidase activation is driven by TXNIP which subsequently triggers NALP3 inflammasome activation in podocytes and ultimately led to podocyte injury PMID: 25834832
Suggest that TXNIP plays a critical role in anti-Her-1/Her-2 treatment and may be a potential prognostic marker in breast cancer. PMID: 25605021
The expression of TXNIP was significantly higher in normal-weight type- 2 diabetic patients than in obese ones. PMID: 25726203
The inhibition of TRX by TXNIP is mediated by an intermolecular disulphide interaction resulting from a novel disulphide bond-switching mechanism. This bond dissociates in the presence of high concentrations of reactive oxygen species. PMID: 24389582
The protein expression level of TXNIP was negatively correlated with the level of miR-373 in MCF-7 cells and in breast cancer tissue of various migration and invasion abilities. TXNIP was regulated by miR-373. PMID: 26122224
data suggest that loss of the p53 tumor suppressor cooperates with Mychigh/TXNIPlow-driven metabolic dysregulation to drive the aggressive clinical behavior of TNBC. PMID: 25870263
diabetes was associated with a >30-fold increase in TxnIP gene expression and a 17 % increase in TxnIP protein expression. PMID: 24925443
TXNIP has been discovered to control pancreatic B-cell microRNA expression, B-cell function, and insulin production. [Review] PMID: 24911120
regulatory relationship between mTOR and the MondoA-TXNIP axis that we propose contributes to glucose homeostasis PMID: 25332233

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Subcellular Location

Cytoplasm.

Protein Families

Arrestin family

Database Links

HGNC: 16952

OMIM: 606599

KEGG: hsa:10628

STRING: 9606.ENSP00000358323

UniGene: Hs.533977

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Code	CSB-RA949793A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: THP-1 whole cell lysate(30µg), K562 whole cell lysate(30µg) All lanes: TXNIP antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 44 kDa kDa Observed band size: 53 kDa kDa Exposure time：2min IHC image of CSB-RA949793A0HU diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing K562 cells stained with CSB-RA949793A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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TXNIP Recombinant Monoclonal Antibody

Product Details

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Target Background