CLYBL Antibody

Datasheet

Code	CSB-PA850772LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, THP-1 whole cell lysate, HepG2 whole cell lysate, NIH/3T3 whole cell lysate All lanes: CLYBL antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 38, 34 KDa Observed band size: 38 KDa Exposure time：5min IHC image of CSB-PA850772LA01HU diluted at 1:50 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37℃ Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) CLYBL Polyclonal antibody

Uniprot No.

Q8N0X4

Target Names

CLYBL

Alternative Names

CLYBL antibody; CLB antibody; Citramalyl-CoA lyase antibody; mitochondrial antibody; EC 4.1.3.25; antibody; 3S)-malyl-CoA thioesterase antibody; EC 3.1.2.30 antibody; Beta-methylmalate synthase antibody; EC 2.3.3.- antibody; Citrate lyase subunit beta-like protein antibody; Citrate lyase beta-like antibody; Malate synthase antibody; EC 2.3.3.9 antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human Citrate lyase subunit beta-like protein, mitochondrial protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The CLYBL Antibody (Product code: CSB-PA850772LA01HU) is Non-conjugated. For CLYBL Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA850772LB01HU	CLYBL Antibody, HRP conjugated	ELISA
FITC	CSB-PA850772LC01HU	CLYBL Antibody, FITC conjugated
Biotin	CSB-PA850772LD01HU	CLYBL Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

Affinity purification

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Mitochondrial citramalyl-CoA lyase indirectly involved in the vitamin B12 metabolism. Converts citramalyl-CoA into acetyl-CoA and pyruvate in the C5-dicarboxylate catabolism pathway. The C5-dicarboxylate catabolism pathway is required to detoxify itaconate, a vitamin B12-poisoning metabolite. Also acts as a malate synthase in vitro, converting glyoxylate and acetyl-CoA to malate. Also displays malyl-CoA thioesterase activity. Also acts as a beta-methylmalate synthase in vitro, by mediating conversion of glyoxylate and propionyl-CoA to beta-methylmalate. Also has very weak citramalate synthase activity in vitro.

Gene References into Functions

Study reports that CLYBL loss leads to a cell-autonomous defect in the mitochondrial B12 metabolism and that itaconyl-CoA is a cofactor-inactivating, substrate-analog inhibitor of the mitochondrial B12-dependent methylmalonyl-CoA mutase PMID: 29056341

Subcellular Location

Mitochondrion.

Protein Families

HpcH/HpaI aldolase family, Citrate lyase beta subunit-like subfamily

Database Links

HGNC: 18355

OMIM: 609686

KEGG: hsa:171425

STRING: 9606.ENSP00000342991

UniGene: Hs.655642

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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