Product Details

Purity

>97% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood T-lymphocytes is in a concentration range of 10- 100 ng/ml.

Target Names

CXCL9

Uniprot No.

Q07325

Research Area

Immunology

Alternative Names

C-X-C motif chemokine 9; chemokine (C-X-C motif) ligand 9; CMK; crg-10; CXCL9; CXCL9_HUMAN; gamma interferon induced monokine; Gamma-interferon-induced monokine; HuMIG; MIG; monokine induced by gamma interferon; monokine induced by interferon gamma; Monokine induced by interferon-gamma; SCYB9; Small inducible cytokine B9; small inducible cytokine subfamily B member 9; Small-inducible cytokine B9

Species

Homo sapiens (Human)

Source

E.Coli

Expression Region

23-125aa

Complete Sequence

TPVVRKGRCS CISTNQGTIH LQSLKDLKQF APSPSCEKIE IIATLKNGVQ TCLNPDSADV KELIKKWEKQ VSQKKKQKNG KKHQKKKVLK VRKSQRSRQK KTT

Mol. Weight

11.7 kDa

Protein Length

Full Length of Mature Protein

Tag Info

Tag-Free

Form

Liquid or Lyophilized powder

Buffer

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 50 mM NaCl.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

5-10 business days

Datasheet & COA

Please contact us to get it.

Description

The Recombinant Human CXCL9 protein is an indispensable research tool for scientists working in the field of immunology. This C-X-C motif chemokine 9, known by its aliases CXCL9, CMK, MIG, and SCYB9, is expressed in E. coli and spans the 23-125aa region, covering the full length of the mature protein. Supplied as a tag-free, lyophilized powder, this protein can be easily reconstituted with sterile water or an appropriate buffer to suit a wide range of experimental requirements.

Our Recombinant Human CXCL9 protein demonstrates a high purity of over 97%, as established by SDS-PAGE and HPLC analyses. Endotoxin levels are meticulously controlled, ensuring that they remain below 1.0 EU/µg as verified by the LAL method. The protein is fully biologically active, as demonstrated by its efficacy in a chemotaxis bioassay using human peripheral blood T-lymphocytes, with a functional concentration range of 10-100 ng/ml.

Several studies have emphasized the importance of CXCL9 in immunology research. For example, Luster et al. (1988)^[1] identified the interferon-γ-induced protein 10, later known as CXCL9, and described its role as a chemoattractant for monocytes and T cells. Moreover, Groom and Luster (2011)^[2] highlighted the diverse roles of CXCL9 in regulating immune responses and controlling infections. Most recently, Hirahara et al. (2020)^[3] discussed the potential of CXCL9 as a biomarker and therapeutic target in autoimmune diseases. These studies underscore the significance of CXCL9 in the immune system and hint at its possible application in the treatment of immune-related diseases.

References:
1. Luster AD, Unkeless JC, Ravetch JV. γ-Interferon transcriptionally regulates an early-response gene containing homology to platelet proteins. Nature. 1988;334(6179):265-8.
2. Groom JR, Luster AD. CXCR3 in T cell function. Exp Cell Res. 2011;317(5):620-31.
3. Hirahara K, et al. Development of novel immunotherapies targeting type 1 cytokines and CXCR3. Ann Rheum Dis. 2020;79(2):157-8.

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Target Background

Function

Cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. Chemotactic for activated T-cells. Binds to CXCR3.

Gene References into Functions

CXC chemokine ligand 9 promotes the progression of diffuse large B-cell lymphoma in a beta-catenin-dependent manner. PMID: 30130730
CXCL9 level was significantly higher in alopecia areata patients compared with controls and with vitiligo patients PMID: 27863059
CXCL9 may promote prostate cancer progression via inhibition of cytokines from T cells. PMID: 29901197
High CXCL9 expression is associated with invasion and metastasis in tongue squamous cell carcinoma. PMID: 29286143
MIG low in the tumor microenvironment can be used as potential indicators for the progression of non-small cell lung cancer PMID: 28375674
the results of the current study revealed increased serum levels of MIG as a novel independent marker of poor prognosis in newly diagnosed patients with MM. PMID: 26999330
High CXCL9 expression is associated with clear-cell renal cell carcinoma. PMID: 26910919
Circulating CXCL9 serum levels are high in chronic Q fever patients and is a promising biomarker for the diagnosis of chronic Q fever. PMID: 28793883
High urine CXCL9 level is associated with acute rejection in Histologically Stable Kidney transplant Recipients. PMID: 26694099
CXCL9 expression in lesional macrophages implicates the skin as the source of circulating cytokines. CXCL9 is a promising biomarker of disease activity in morphea. PMID: 28450066
significant correlation between serum levels and the severity of coronary artery disease, quantified by the Gensini score PMID: 28100872
High CXCL9 plasma levels favour response to pegIFN alpha 2a and ribavirin in hepatitis C virus infected patients regardless of DPP4 activity. PMID: 26344576
CXCL9 is a useful predictor of better clinical outcome in colorectal cancer patients. PMID: 26898419
The mRNA expression and serum levels of CXCL9 were both increased. PMID: 26828996
Data show that asymptomatic patients with unstable plaques exhibited higher levels of endothelial microparticles (EMPs), CXCL9 chemokine and stem cell growth factor; lymphocyte secreted C-type lectin (SCGF-beta) compared to those with stable plaques. PMID: 26564003
congruent with the concept that inflammation plays a key role in the pathogenesis of LV dysfunction, MIG, IP10 and I-TAC add diagnostic accuracy over and beyond NT-pro BNP. PMID: 26506526
Suggest that CD44 and CXCL9 may serve as predictive biomarkers to identify liver allograft recipients at risk for clinically significant acute graft rejection. PMID: 25950774
Knock-down of CD44 resulted in an upregulation of mRNA expression of the chemokines CXCL9 and CXCL12, as well as their receptors CXCR3 and CXCR4. PMID: 24614402
There were no significant differences in distribution of CXCL9 genotypes and alleles between rheumatoid arthritis patients and control group. PMID: 25702175
This work confirms that OPN, CCL5 and CXCL9 plasma levels are higher in psoriasis patients and provides evidence that their higher levels are not a consequence of obesity. PMID: 25972108
This inhibitory action of resveratrol was also observed for the cytokines-induced expression of chemokines CXCL9, CCL2 and CCL5. PMID: 25890876
This paper suggests a role for Epithelial to mesenchymal cell transition in the pathogenesis of idiopathic pulmonary fibrosis and provides a novel mechanism for the inhibitory effects of CXCL9 on TGF-beta1-induced Epithelial to mesenchymal cell transition. PMID: 26268659
The expression of TNF-alpha and CXCL9 in blood samples stimulated with a bacterial antigen distinguishes active tuberculosis patients from latent disease carriers and healthy controls. PMID: 25528189
CXCL9 inhibits the proliferation of epithelial cells via phosphorylation of p70S6K, resulting in the excretion of TGF-beta as downstream mediator. CXCL9/CXCR3 interaction can exacerbate antineoplastic agent-induced intestinal damage. PMID: 25398650
CXCL9 is involved in the invasion ability of hepatocellular carcinoma cells possibly through up-regulation of its potential effector PREX2. PMID: 25151370
both bactericidal and cell-recruiting activities of MIG/CXCL9 are corrupted by P. aeruginosa through release of elastase, and this may contribute to impaired airway host defense in CF. PMID: 25115612
Elevated IL-2R, IL-1RA, and CXCL9 are associated with shorter event-free survival in newly diagnosed follicular lymphoma, treated with chemoimmunotherapy. PMID: 25422100
CXCL9 expression was low in control skin, mild in perilesional skin from slowly progressive vitiligo and high in progressive vitiligo. Such expression was found in the epidermal as well as the dermal infiltrate. PMID: 24438589
study demonstrated that CXCL9 is associated with tumor burden and aggressiveness of NPC tumors and the serum level of this ligand may be useful as a prognostic indicator PMID: 24278236
Higher circulating levels of CXCL-9 and -11 have been shown in non-pregnant autoimmune thyroiditis patients with a history of recurrent abortion as compared to both control groups PMID: 24351073
In this study of human cryoglobulinemic glomerulonephritis we showed a striking expression of cytokine CXCL9, a classical macrophage activation marker, by the macrophages and possibly other cell types within the glomeruli. PMID: 24084359
High urinary CXCL9 is associated with kidney transplant injury. PMID: 23968332
CXCL9 associated with sustained virological response in chronic hepatitis B patients receiving peginterferon alfa-2a therapy. PMID: 24124595
Plasma concentrations of CXCL9 are elevated at the onset of chronic graft-versus-host disease diagnosis, but not in patients with chronic graft-versus-host disease for more than 3 months. PMID: 24363401
Collectively, our results show that a diverse milieu of chemokines is expressed in myocardium and valvular tissue lesions and emphasize the role of CXCL9/Mig in mediating T cell recruitment to the site of inflammation in the heart. PMID: 23417848
Our study demonstrated that CXCL9 is associated with tumor burden and aggressiveness of OSCC tumors and serum level of this ligand may be useful as a prognostic indicator PMID: 23769451
CXCL9 was increased in lung transplant lavage fluid during diffuse alveolar damage, acute rejection, and lymphocytic bronchiolitis. Elevated CXCL9 was associated with increased risk of chronic allograft dysfunction. PMID: 24063316
Our study demonstrates in mixed cryoglobulinemia and hepatitis C vs controls: (i)high serum CXCL9 and CXCL11, significantly associated with the presence of active vasculitis; (ii) a strong relationship between circulating CXCL9 and CXCL11 PMID: 23527708
The secretion of CXCL9 by activated T cells is also increasingly downregulated with increasing concentrations of bortezomib. PMID: 23179902
Report high serum levels of CXCL9/TNF-alpha/interferon-gamma in patients with mixed cryoglobulinaemia and chronic hepatitis C. PMID: 22766105
Intragraft CXCL9 mRNA has a functionally important role in T-cell activation in liver allograft and serves as biomarker for acute cellular rejection. PMID: 22889476
CXCL9 is localized predominantly in the cytoplasm of breast cancer cells and prostaglandin E2 inhibits cytokine induced CXCL9 secretion. PMID: 22333315
Malaria-infected primigravidae with placental CXCL9 levels in the lowest tertile gave birth to babies who weighed 610 g more than babies born to mothers with high CXCL9 levels. PMID: 22689822
Elevated plasma levels of MIG are associated with inflammatory bowel disease with JCV infection. PMID: 22467521
HSV-2 induces CXCL9 expression in human cervical epithelial cells by activation of p38-C/EBP-beta pathway PMID: 22586042
Autophagy may be a crucial intracellular mechanism of Mig induction in response to HBV infection. PMID: 22580676
Immunohistochemically, the lining synovium of Rheumatoid Aarthritis clearly expressed CXCL9 PMID: 22401175
Elevated plasma levels of interferon-gamma and Cys-X-Cys chemokine receptor 3-binding chemokine CXCL9 are present in patients with thoracic aortic aneurysms. PMID: 21962843
We found that urine CXCL9 and CXCL10 were markedly elevated in adults and children experiencing either AR or BKI (p = 0.0002), but not in stable allograft recipients PMID: 21812928
We first show that circulating CXCL9 and CXCL11 are increased in patients with thyroiditis and hypothyroidism and are related to each other. PMID: 21470996