Recombinant Rat C-X-C motif chemokine 2 protein (Cxcl2), partial (Active)

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Code CSB-AP001401RA
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Size $142
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Product Details

Purity
>98% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using rat neutrophils is in a concentration range of 10-100 ng/ml.
Target Names
Cxcl2
Uniprot No.
Research Area
Immunology
Alternative Names
Cxcl2; Cinc3; Mip-2; Mip2; Scyb2C-X-C motif chemokine 2; Cytokine-induced neutrophil chemoattractant 3; CINC-3; Macrophage inflammatory protein 2; MIP2
Species
Rattus norvegicus (Rat)
Source
E.coli
Expression Region
28-100aa
Complete Sequence
VVVASELRCQ CLTTLPRVDF KNIQSLTVTP PGPHCAQTEV IATLKDGHEV CLNPEAPLVQ RIVQKILNKG KAN
Mol. Weight
7.9 kDa
Protein Length
Partial
Tag Info
Tag-Free
Form
Lyophilized powder
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
5-10 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
Chemotactic for human polymorphonuclear leukocytes but does not induce chemokinesis or an oxidative burst. Contributes to neutrophil activation during inflammation.
Gene References into Functions
  1. this study shows elevated expression of CINC-3 at the site of inflammation as well as their significant reflection in the circulation, thereby suggesting their frontline role in carrageenan-induced acute inflammation PMID: 27967265
  2. rutin is a potential protective agent in spinal cord injury enhancing the neurotrophic effect by inhibiting the expression of MIP-2 and activation of MMP-9, and downregulating the expression of p-Akt. PMID: 26502930
  3. The rat spiral ligament fibrocytes were found to release CXCL2 in response to nontypeable H. influenzae via activation of c-Jun, leading to the recruitment of polymorphonuclear cells to the cochlea. PMID: 22379036
  4. CsA has no significant effect on serum levels of MCP-1 and MIP-2 following renal transplantation in rats. PMID: 20034917
  5. Results identify FQHPSFI peptide may be used for the modulation of lipopolysaccharide-stimulated MIP-2 production in alveolar macrophagess. PMID: 20186460
  6. chemokine production by rat alveolar macrophages is inhibited by taurine chloramine PMID: 11716962
  7. Intratracheal LPS induced a significant increase in MIP-2 in bronchoalveolar lavage (BAL) fluid with no detectable MIP-2 in the plasma. PMID: 11837784
  8. demonstrated that the beta-glucan component of the Pneumocystis carinii cell wall is able to stimulate alveolar epithelial cells to produce MIP-2 PMID: 11906040
  9. role for the nitric oxide pathway in the overproduction of pro-inflammatory mediators IL-6 and MIP-2 during GBS-induced lung inflammation. PMID: 12661899
  10. junctional epithelium cells produced MIP-2 and CINC-2 in response to LPS stimulation, suggesting that MIP-2 and CINC-2 may be responsible for PMN migration toward the periodontal pathogen and may play an important role in the initiation of inflammation PMID: 15045510
  11. macrophage inflammatory protein 2 mediates platelet-activating factor-induced intestinal injury PMID: 15319184
  12. no significant differences were detected for MIP-2 levels in diabetic rats when compared with non-diabetic rats after hypoxia PMID: 16132691
  13. Following cecal ligation and puncture Cxcl2 production increases in the lung, setting the stage for neutrophil accumulation in lung during sepsis. PMID: 16818791
  14. MIP-2/CXCL2 and MCP-1/CCL2 are increased after injury, and neurons appear to be the source of this expression. Chemokine expression was selectively inhibited by dexamethasone. PMID: 19210118

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Subcellular Location
Secreted.
Protein Families
Intercrine alpha (chemokine CxC) family
Tissue Specificity
At least expressed in the lung and trachea.
Database Links
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