Rat macrophage inflammatory protein 2,MIP-2 ELISA kit

Code CSB-E07419r
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

The rat MIP-2 ELISA Kit is intended to measure rat MIP-2 in serum, plasma, or tissue homogenates. This assay uses the bi-antibody sandwich technique and enzyme-substrate chromogenic reaction to quantify rat MIP-2 concentration. The intensity of the colored product is directly proportional to the concentration of MIP-2 present in the sample. This rat MIP-2 ELISA kit has high specificity and excellent sensitivity.

MIP-2, also called CXCL2, is a peptide derived from monocytes/ macrophages that attracts and recruits neutrophils to inflamed sites through the p38 MAPK-dependent signaling pathway, by binding to CXCR1 and CXCR2. CXCL2-recruited and activated neutrophils can accelerate liver inflammation by releasing various inflammatory mediators. CXCL2 expression is increased during hepatic I/R injury. Some studies have demonstrated that CXCL2 is overexpressed in breast, colon, prostate, and bladder cancers. While CXCL2 is down-regulated in HCC cell lines, inhibiting hepatocellular carcinoma (HCC) cell proliferation in vitro.

Target Name chemokine (C-X-C motif) ligand 2
Alternative Names Cxcl2 ELISA kit; Cinc3 ELISA kit; Mip-2 ELISA kit; Mip2 ELISA kit; Scyb2C-X-C motif chemokine 2 ELISA kit; Cytokine-induced neutrophil chemoattractant 3 ELISA kit; CINC-3 ELISA kit; Macrophage inflammatory protein 2 ELISA kit; MIP2 ELISA kit
Abbreviation CXCL2
Uniprot No. P30348
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 78 pg/mL-5000 pg/mL
Sensitivity 19.5 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Linearity
Recovery
Typical Data
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

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Target Background

Function
(From Uniprot)
Chemotactic for human polymorphonuclear leukocytes but does not induce chemokinesis or an oxidative burst. Contributes to neutrophil activation during inflammation.
Gene References into Functions
  1. this study shows elevated expression of CINC-3 at the site of inflammation as well as their significant reflection in the circulation, thereby suggesting their frontline role in carrageenan-induced acute inflammation PMID: 27967265
  2. rutin is a potential protective agent in spinal cord injury enhancing the neurotrophic effect by inhibiting the expression of MIP-2 and activation of MMP-9, and downregulating the expression of p-Akt. PMID: 26502930
  3. The rat spiral ligament fibrocytes were found to release CXCL2 in response to nontypeable H. influenzae via activation of c-Jun, leading to the recruitment of polymorphonuclear cells to the cochlea. PMID: 22379036
  4. CsA has no significant effect on serum levels of MCP-1 and MIP-2 following renal transplantation in rats. PMID: 20034917
  5. Results identify FQHPSFI peptide may be used for the modulation of lipopolysaccharide-stimulated MIP-2 production in alveolar macrophagess. PMID: 20186460
  6. chemokine production by rat alveolar macrophages is inhibited by taurine chloramine PMID: 11716962
  7. Intratracheal LPS induced a significant increase in MIP-2 in bronchoalveolar lavage (BAL) fluid with no detectable MIP-2 in the plasma. PMID: 11837784
  8. demonstrated that the beta-glucan component of the Pneumocystis carinii cell wall is able to stimulate alveolar epithelial cells to produce MIP-2 PMID: 11906040
  9. role for the nitric oxide pathway in the overproduction of pro-inflammatory mediators IL-6 and MIP-2 during GBS-induced lung inflammation. PMID: 12661899
  10. junctional epithelium cells produced MIP-2 and CINC-2 in response to LPS stimulation, suggesting that MIP-2 and CINC-2 may be responsible for PMN migration toward the periodontal pathogen and may play an important role in the initiation of inflammation PMID: 15045510
  11. macrophage inflammatory protein 2 mediates platelet-activating factor-induced intestinal injury PMID: 15319184
  12. no significant differences were detected for MIP-2 levels in diabetic rats when compared with non-diabetic rats after hypoxia PMID: 16132691
  13. Following cecal ligation and puncture Cxcl2 production increases in the lung, setting the stage for neutrophil accumulation in lung during sepsis. PMID: 16818791
  14. MIP-2/CXCL2 and MCP-1/CCL2 are increased after injury, and neurons appear to be the source of this expression. Chemokine expression was selectively inhibited by dexamethasone. PMID: 19210118

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Subcellular Location Secreted.
Protein Families Intercrine alpha (chemokine CxC) family
Tissue Specificity At least expressed in the lung and trachea.
Database Links

KEGG: rno:114105

STRING: 10116.ENSRNOP00000003745

UniGene: Rn.10230

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