DDB2 Antibody

Code CSB-PA740641XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) DDB2 Polyclonal antibody
Uniprot No.
Target Names
DDB2
Alternative Names
DDB2 antibody; At5g58760 antibody; MZN1.20Protein DAMAGED DNA-BINDING 2 antibody; UV-damaged DNA-binding protein 2 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana DDB2 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
May function as the substrate recognition module for a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex including DDB1A and CUL4. Required for DNA repair. Binds to DDB1A to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. Involved in UV-B tolerance and genome integrity. In association with ATCSA-1, is necessary for repair of UV-B-induced DNA lesions.
Gene References into Functions
  1. DDB2 role in the repair of UV-induced DNA lesions PMID: 28325872
  2. DDB2 but not ATCSA-1 expression is upregulated upon UV treatment. PMID: 20128879
  3. AtUV-DDB2 functions in DNA repair and has a direct or indirect influence on cell proliferation in the plant meristem. [UV-DDB2] PMID: 16786303
Subcellular Location
Nucleus. Note=Broadly distributed over chromatin.
Protein Families
WD repeat DDB2/WDR76 family
Tissue Specificity
Expressed in roots and flowers. Expressed at low levels in stems.
Database Links

KEGG: ath:AT5G58760

STRING: 3702.AT5G58760.1

UniGene: At.29266

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