TOP1 Antibody

1. Studies demonstrate opposing roles of Top1 activity in either promoting or destabilizing genome integrity. Resolution of transcription-associated negative supercoils, for example, prevents pairing of the nascent RNA with the DNA template (R-loops) as well as DNA secondary structure formation. Reduced Top1 levels thus enhance CAG repeat contraction, somatic hypermutation, and class switch recombination. PMID: 29177731
2. Our findings reveal a functional interplay between Mms21 and Top1 in maintenance of longer chromosomes, and suggest that lack of sumoylation of Mms21 targets coupled with Top1 deficiency is a crucial requirement for accurate inheritance of longer chromosomes. PMID: 27872982
3. Too much, as well as too little of Top1 is detrimental to eukaryotic genomes, and that CPT has destabilizing effects that extend beyond those associated with DSB formation. PMID: 28961461
4. The authors demonstrate biochemically that irreversible double-strand breaks are generated by subsequent Top1 cleavage on the opposite strand from the Top1-induced DNA nicks at ribonucleotide sites. PMID: 27932446
5. The role of topoisomerase I in suppressing genome instability associated with a highly transcribed guanine-rich sequence is not restricted to preventing RNA:DNA hybrid accumulation. PMID: 26527723
6. top1 mutants had greatly elevated levels of instability within the tandemly-repeated ribosomal RNA genes. PMID: 25830313
7. Top1 is a critical factor in suppression of instability associated with potential G4 DNA-forming sequences. PMID: 25473964
8. analysis of how human and yeast DNA topoisomerase I domain interactions impact enzyme activity and sensitivity to camptothecin PMID: 25795777
9. Top1 reversibly cleaves genomic ribonucleotides with formation of a ribonucleotide 2',3'-cyclic phosphate.Top1 cleaves two nucleotides upstream of the ribonucleotide 2',3'-cyclic phosphate to yield a covalent Top1-DNA complex. PMID: 25777529
10. Top1-dependent deletions are initiated either by Top1 cleavage complex trapping or by Top1 incision at ribonucleoside monophosphates in DNA. PMID: 23305949
11. study demonstrates that rNMP (ribonucleoside monophosphates)-associated deletion formation requires the activity of Top1; studies extend the role of Top1 to include the processing of rNMPs in genomic DNA into irreversible single-strand breaks PMID: 21700875
12. analysis of unavoidable conflicts between Top1p and the transcriptional machinery and their potential impact on genome stability PMID: 21177431
13. Loss of Top1 enhances inherent R-loop formation, particularly over the 5' region of the rDNA, imposing persistent transcription blocks when RNase H is limiting. PMID: 20634320
14. independent alterations in DNA cleavage and religation contribute to the lethal phenotype of DNA topoisomerase I active site mutations PMID: 15489506
15. Data suggest that topoisomerase II is the main modulator of DNA topology in chromatin fibers, and that topoisomerase I then assists DNA relaxation where chromatin structure impairs DNA juxtaposition but allows twist diffusion. PMID: 16710299
16. Changes in the flexibility or orientation of the linker alter the geometry of the active site and thereby the kinetics of DNA cleavage/religation. PMID: 17276985
17. data are consistent with a coordinated action of Top1 and Top2 in counteracting the accumulation of torsional stress and sister chromatid entanglement at replication forks PMID: 17671091
18. Top1p can optimize transcript levels for cell growth in exponentially growing cells under a synthetic medium with glucose PMID: 18272174
19. Disulfide cross-links reveal conserved features of DNA topoisomerase I architecture and a role for the N terminus in clamp closure PMID: 18693244

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KEGG: sce:YOL006C

STRING: 4932.YOL006C