Bovine Interferon β,IFN-β/IFNB ELISA Kit

Code CSB-E13699B
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Alternative Names
IFNB1Interferon beta-1 ELISA Kit
Abbreviation
IFNB1
Uniprot No.
Species
Bos taurus (Bovine)
Sample Types
Detection Range
Sensitivity
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine IFNB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 92  
Range % 83-107  
1:2 Average % 89  
Range % 86-98  
1:4 Average % 94  
Range % 83-102  
1:8 Average % 93  
Range % 82-105  
Recovery
The recovery of bovine IFNB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 89-100  
EDTA plasma (n=4) 95 86-104  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
200 0.134 0.138 0.136    
80 0.241 0.225 0.233    
48 0.324 0.305 0.315    
20 0.586 0.618 0.602    
8 1.253 1.234 1.244    
0 2.598 2.671 2.635    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
(From Uniprot)
Has antiviral, antibacterial and anticancer activities.
Gene References into Functions
  1. BVDV2-E significantly increased IFN-beta activity compared to BVDV2-wt. PMID: 28432927
  2. The data confirm the involvement of EHMT2 in the epigenetic regulation of IFN-b and demonstrate the activation of a general antiviral state after EHMT2 inhibition. PMID: 26418342
  3. The authors provide evidence that ICP27 protein encoded by bovine herpesvirus type 1, a viral early protein that shuttles between the nucleus and cytoplasm inhibits transcriptional activity of two bovine IFN-beta gene promoters (IFN-beta1 and IFN-beta3). PMID: 22902481
  4. The authors demonstrate that bovine herpesvirus 1 bICP0 effectively inhibits bovine IFN-beta promoter activity and induces IRF3 degradation. PMID: 21689696
  5. These studies provide evidence that virus infection differentially stimulates expression of the three bovine IFN-beta genes. PMID: 21316405
  6. recombinant bovine respiratory syncytial virus lacking the NS proteins, and those lacking NS2 in particular, are strong inducers of IFN-alpha/beta in bovine nasal fibroblasts and bronchoalveolar macrophages. PMID: 12857912

Show More

Hide All

Subcellular Location
Secreted.
Protein Families
Alpha/beta interferon family
Database Links
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1