Recombinant Bovine Interferon beta-1(IFNB1)

Code CSB-YP011048BO
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Source Yeast
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Code CSB-EP011048BO
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Source E.coli
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Code CSB-EP011048BO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP011048BO
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Source Baculovirus
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Code CSB-MP011048BO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names IFNB1
Uniprot No. P01578
Alternative Names IFNB1Interferon beta-1
Species Bos taurus (Bovine)
Expression Region 22-186
Target Protein Sequence RSYSLLRFQ QRQSLKECQK LLGQLPSTSQ HCLEARMDFQ MPEEMKQEQQ FQKEDAILVM YEVLQHIFGI LTRDFSSTGW SETIIEDLLK ELYWQMNRLQ PIQKEIMQKQ NSTTEDTIVP HLGKYYFNLM QYLESKEYDR CAWTVVQVQI LTNVSFLMRL TGYVRD
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
(From Uniprot)
Has antiviral, antibacterial and anticancer activities.
Gene References into Functions
  1. BVDV2-E significantly increased IFN-beta activity compared to BVDV2-wt. PMID: 28432927
  2. The data confirm the involvement of EHMT2 in the epigenetic regulation of IFN-b and demonstrate the activation of a general antiviral state after EHMT2 inhibition. PMID: 26418342
  3. The authors provide evidence that ICP27 protein encoded by bovine herpesvirus type 1, a viral early protein that shuttles between the nucleus and cytoplasm inhibits transcriptional activity of two bovine IFN-beta gene promoters (IFN-beta1 and IFN-beta3). PMID: 22902481
  4. The authors demonstrate that bovine herpesvirus 1 bICP0 effectively inhibits bovine IFN-beta promoter activity and induces IRF3 degradation. PMID: 21689696
  5. These studies provide evidence that virus infection differentially stimulates expression of the three bovine IFN-beta genes. PMID: 21316405
  6. recombinant bovine respiratory syncytial virus lacking the NS proteins, and those lacking NS2 in particular, are strong inducers of IFN-alpha/beta in bovine nasal fibroblasts and bronchoalveolar macrophages. PMID: 12857912

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Subcellular Location Secreted.
Protein Families Alpha/beta interferon family
Database Links

KEGG: bta:281845

STRING: 9913.ENSBTAP00000055343

UniGene: Bt.45040

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