Bovine Interleukin 1β (IL- 1β) ELISA kit

Bovine Interleukin 1β (IL- 1β) ELISA kit

Product Details

Citations

Customer Reviews and Q&A

Target Background

Product Details

Citations

Related Products

Customer Reviews and Q&A

Target Background

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Bovine Interleukin 1β (IL- 1β) ELISA kit

Citations (23) Protocol MSDS

Code	CSB-E12986B
Size	96T,5×96T,10×96T
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Product Details
Citations (23)
Related Products
Customer Reviews and Q&A
Target Background

Target Name

interleukin 1, beta

Alternative Names

IL1B ELISA Kit; Interleukin-1 beta ELISA Kit; IL-1 beta ELISA Kit

Abbreviation

IL1B

Uniprot No.

P09428

Species

Bos taurus (Bovine)

Sample Types

serum, plasma, tissue homogenates

Detection Range

62.5 pg/mL-4000 pg/mL

Sensitivity

15.6 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	99
	Range %	95-108
1:2	Average %	94
	Range %	91-98
1:4	Average %	92
	Range %	88-95
1:8	Average %	87
	Range %	81-93

Recovery

The recovery of bovine IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-102
EDTA plasma (n=4)	92	87-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	2.876	2.862	2.869	2.751
2000	2.444	2.439	2.442	2.324
1000	1.903	1.856	1.880	1.762
500	1.412	1.403	1.408	1.290
250	1.164	1.185	1.175	1.057
125	0.809	0.799	0.804	0.686
62.5	0.471	0.479	0.475	0.357
0	0.117	0.118	0.118

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Bovine IL1B ELISA Kit was designed for the quantitative measurement of Bovine IL1B protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL .

Digital dermatitis in the dairy cow H Pirkkalainen,/,/
Immunological response enhancement in cows with subclinical mastitis fed diet supplemented with Macleaya cordata MSA Khattab, L Haiqiang, T Shaoxun,Frontiers in Veterinary Science,2025
Dynamics of oxidative stress and immune responses in neonatal calves during diarrhea ZL Fu,Journal of dairy science,2024
Concentrations of serum amyloid A, haptoglobin and some cytokines in calves with Cryptosporidiosis in the pre-and post-treatment stage K Mustafa ,Journal of Advances in VetBio Science and Techniques,2023
Ovarian activity, hormone profile, pro‐inflammatory cytokines and reproductive performance of buffalo cows fed diets with different estrogenicity NM Hashem,Journal of animal physiology and animal nutrition,2023
Selenium attenuates S. aureus-induced inflammation by regulation TLR2 signaling pathway and NLRP3 inflammasome in RAW 264.7 macrophages MJ Wei,Biological trace element research,2023
Overexpressed SIRT6 attenuates cisplatin-induced acute kidney injury by inhibiting ERK1/2 signaling Li Z,Kidney international,2023
Local and Systemic Inflammation in Finnish Dairy Cows with Digital Dermatitis YS Wang,Animals,2024
Comparative Study of Mycobacterium bovis and Mycobacterium avium subsp. paratuberculosis In Vitro Infection in Bovine Bone Marrow Derived Macrophages: Preliminary Results H Hu,Microorganisms,2024
The relationship between serum cytokine profile and vitamin D in calves with neonatal diarrhea M Caliskan,Cytokine,2023
Dimethyl itaconic acid improves viability and steroidogenesis and suppresses cytokine production in LPS-treated bovine ovarian granulosa cells by regulating TLR4/nfkβ, NLRP3, JNK signaling pathways MR Tabandeh,Research in veterinary science,2022
lncRNA MALAT1 Promotes Diabetic Nephropathy Progression via miR-15b-5p/TLR4 Signaling Axis Z Yang,Journal of Immunology Research,2022
Faecal microbiota in two-week-old female dairy calves during acute cryptosporidiosis outbreak–Association with systemic inflammatory response E Dorbek-Kolin,Research in Veterinary Science,2022
Determining Serum Haptoglobin and Proinflammatory Cytokines Concentration in the Calves Clinically Diagnosed with Pneumonitis, Pneumoenteritis and Enteritis M. KABU,amazonaws.com,2022
Ginsenoside Compound K Through TGR5/YAP Signal To Increase Glucagon-Like Peptide-1 Release and L-Cell Number in db/db Mice Fengyuan Tian,SSRN,2022
Effect of colostrum on the acute-phase response in neonatal dairy calves K Peetsalu,Journal of dairy science,2022
Caffeic Acid Prevented LPS-Induced Injury of Primary Bovine Mammary Epithelial Cells through Inhibiting NF-κB and MAPK Activation Mingjiang Liu,Mediators of Inflammation,2019
Effects of continuous low dose infusion of lipopolysaccharide on inflammatory responses, milk production and milk quality in dairy cows Ning LT.et al,J Anim Physiol Anim Nutr ,2017
Concentrations of serum amyloid A, haptoglobin, tumour necrosis factor and interleukin-1 and -6 in Anatolian buffaloes naturally infected with dermatophytosis M. Kabu.et al,Veterinarni Medicina,2016
Concentrations of serum amyloid A, haptoglobin,tumour necrosis factor and interleukin-1 and -6 in Anatolian buffaloes naturally infected with dermatophytosis Kabu M,.et al,Veterinarni Medicina,2016
Alterations in innate immunity reactants and carbohydrate and lipid metabolism precede occurrence of metritis in transition dairy cows Elda Dervishi. et al,Research in Veterinary Science,2016
Alterations of Innate Immunity Reactants in Transition Dairy Cows before Clinical Signs of Lameness Guanshi Zhang. et al,Animals,2015
Kinetics of pro-inflammatory cytokines, interleukin-10, and virus neutralising antibodies during acute ephemeral fever virus infections in Brahman cattle Barigye R. et al,Vet Immunol Immunopathol,2015

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Function
(From Uniprot)

Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6.

Gene References into Functions

Mycobacterium bovis infected animals have a higher frequency of IFN-gamma producing CD4(+) T cells and elevated plasma IL-1beta levels. PMID: 28610788
The presence of embryos increased endometrial IL1B protein locally, while no differences regarding IL1R1 protein and IL1B and IL1R1 mRNA were detected. PMID: 25955718
For the inflammasome-dependent IL-1beta release, bovine monocytes require ATP in addition to a primary stimulus. This IL-1beta release depends on potassium efflux, but, in contrast to human and murine monocytes, does not require calcium influx or generation of reaction oxygen and is independent of the P2X7 receptor. PMID: 22728096
Role of TGF-beta1 and TNF-alpha in IL-1beta mediated activation of proMMP-9 in pulmonary artery smooth muscle cells: involvement of an aprotinin sensitive protease. PMID: 21722622
Testicular IL-1 alpha and IL-1 beta concentrations were highest in the early post-natal period; however, IL-1 bioactivity and IL-6 concentrations were greatest in the immediate pre-pubertal period. PMID: 19144032
Data describe the expression of IL-8, IL-1beta and their respective receptors, CXCR1 and IL-1R1 in bovine theca cells, and suggest that VEGF is associated with the IL system in theca cells in ovary. PMID: 20156197
Genes for IL-1alpha and IL-1beta are expressed and a functional IL-1R is present in bovine corpora lutea throughout luteal phase. IL-1alpha and IL-1beta may have different roles as regulating PGF(2alpha) and PGE(2) production during luteal phase. PMID: 15253926
non-metalloproteinase mechanisms participate in IL-1-induced matrix degradation and loss of tissue material properties PMID: 17174540
Lipopolysaccharide stimulation up-regulates the secretion of cytokines by bovine polymorphonuclear neutrophil leukocytes. PMID: 17727806
dynamic compression stimulates cell proliferation and proteoglycan synthesis in the presence of IL-1beta and/or inhibitors of the MAPKs and NFkappaB and AP-1 signalling pathways PMID: 18545934
These results indicate that activation of the intrinsic antistaphylococcal response in bovine endothelial cells (BEC), enhanced by TNF-alpha and IL-1beta, is effective to eliminate S. aureus and S. epidermidis. PMID: 18625014
the low friction of articular cartilage can be modified by TGF-beta1 and IL-1beta treatment and that the friction coefficient depends on multiple factors, including superficial zone protein localization and surface roughness PMID: 18683879
These results suggest that IL-1alpha and IL-1beta are produced by the stromal cells, that IL-1beta is produced by the epithelial cells, and that IL-1alpha is a more potent stimulator of prostaglandin and plasminogen activator in bovine endometrial cells. PMID: 18930361
Data suggest an important regulatory mechanism of interleukin-1beta production, associated with microglial migration and proliferation, driven by ECM degradation and/or synthesis in an injured brain. PMID: 19250967

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Subcellular Location

Cytoplasm, cytosol. Lysosome. Secreted, extracellular exosome. Secreted.

Protein Families

IL-1 family

Database Links

KEGG: bta:281251

STRING: 9913.ENSBTAP00000001745

UniGene: Bt.4856

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Related pathways

MAPK signaling pathway Lipids and Inflammation in Atherogenesis NF-kappa B signaling pathway TNF signaling pathway Necroptosis Hematopoietic cell lineage IL-17 signaling pathway Inflammasome Signaling NOD-like receptor signaling pathway Osteoclast differentiation Th17 cell differentiation Toll-like receptor signaling pathway AGE-RAGE signaling pathway in diabetic complications Excitatory synapse pathway

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.