Target Name
thymus activation regulated chemokine(TARC/CCL17)
Alternative Names
CCL17 ELISA Kit; TARCC-C motif chemokine 17 ELISA Kit; CC chemokine TARC ELISA Kit; Small-inducible cytokine A17 ELISA Kit; Thymus and activation-regulated chemokine ELISA Kit
Species
Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types
serum, plasma, tissue homogenates
Detection Range
23.5 pg/mL-1500 pg/mL
Detection Wavelength
450 nm
Assay Principle
quantitative
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of canine TARC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 87 | |
| Range % | 84-90 | |
| 1:2 | Average % | 102 | |
| Range % | 97-107 | |
| 1:4 | Average % | 91 | |
| Range % | 85-97 | |
| 1:8 | Average % | 95 | |
| Range % | 91-99 | |
Recovery
| The recovery of canine TARC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 96 | 92-99 | |
| EDTA plasma (n=4) | 100 | 97-103 | |
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Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | pg/ml | OD1 | OD2 | Average | Corrected | | | 1500 | 1.908 | 2.005 | 1.957 | 1.801 | | | 750 | 1.179 | 1.205 | 1.192 | 1.036 | | | 375 | 0.734 | 0.738 | 0.736 | 0.580 | | | 187.5 | 0.415 | 0.436 | 0.426 | 0.270 | | | 94 | 0.311 | 0.328 | 0.320 | 0.164 | | | 47 | 0.231 | 0.245 | 0.238 | 0.082 | | | 23.5 | 0.198 | 0.189 | 0.194 | 0.038 | | | 0 | 0.155 | 0.157 | 0.156 | | |
|
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description
This Dog TARC/CCL17 ELISA Kit was designed for the quantitative measurement of Dog TARC/CCL17 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.86 pg/mL.
