Canine thymus activation regulated chemokine(TARC/CCL17)ELISA Kit

Code CSB-E17792c
Size 96T,5×96T,10×96T
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Product Details

Target Name
thymus activation regulated chemokine(TARC/CCL17)
Alternative Names
CCL17 ELISA Kit; TARCC-C motif chemokine 17 ELISA Kit; CC chemokine TARC ELISA Kit; Small-inducible cytokine A17 ELISA Kit; Thymus and activation-regulated chemokine ELISA Kit
Abbreviation
TARC/CCL17
Uniprot No.
Species
Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types
serum, plasma, tissue homogenates
Detection Range
23.5 pg/mL-1500 pg/mL
Sensitivity
5.86 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of canine TARC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %87
Range %84-90
1:2Average %102
Range %97-107
1:4Average %91
Range %85-97
1:8Average %95
Range %91-99
Recovery
The recovery of canine TARC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9692-99
EDTA plasma (n=4)10097-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
15001.908 2.005 1.957 1.801
7501.179 1.205 1.192 1.036
3750.734 0.738 0.736 0.580
187.50.415 0.436 0.426 0.270
940.311 0.328 0.320 0.164
470.231 0.245 0.238 0.082
23.50.198 0.189 0.194 0.038
00.155 0.157 0.156
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Dog TARC/CCL17 ELISA Kit was designed for the quantitative measurement of Dog TARC/CCL17 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.86 pg/mL.

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Target Background

Function
(From Uniprot)
Chemotactic factor for t lymphocytes but not monocytes or granulocytes. May play a role in T-cell development in thymus and in trafficking and activation of mature T-cells. Binds to CCR4 and CCR8.
Gene References into Functions
  1. The present results suggest that TNF-alpha-induced CCL17 mRNA transcription in canine keratinocytes is positively regulated by p38 but negatively controlled by ERK. PMID: 20837364
  2. This study suggests that interferon-gamma may suppress granulocyte-macrophage colony stimulating factor production from canine keratinocytes, although further studies are required to confirm this. PMID: 20860556
  3. Inflammatory cytokines are important inducing factors for the production of CCL17 in the lesional skin of dogs with atopic dermatitis. atopic dermatitis. PMID: 20022349
Subcellular Location
Secreted.
Protein Families
Intercrine beta (chemokine CC) family
Tissue Specificity
Expressed in thymus, spleen, lymph node, lung and heart.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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