Canine thymus activation regulated chemokine(TARC/CCL17)ELISA Kit

Instructions
Code CSB-E17792c
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name thymus activation regulated chemokine(TARC/CCL17)
Alternative Names CCL17 ELISA Kit; TARCC-C motif chemokine 17 ELISA Kit; CC chemokine TARC ELISA Kit; Small-inducible cytokine A17 ELISA Kit; Thymus and activation-regulated chemokine ELISA Kit
Abbreviation TARC/CCL17
Uniprot No. Q95N01
Species Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types serum, plasma, tissue homogenates
Detection Range 23.5 pg/mL-1500 pg/mL
Sensitivity 5.86 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of canine TARC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %87
Range %84-90
1:2Average %102
Range %97-107
1:4Average %91
Range %85-97
1:8Average %95
Range %91-99
Recovery
The recovery of canine TARC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9692-99
EDTA plasma (n=4)10097-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
15001.908 2.005 1.957 1.801
7501.179 1.205 1.192 1.036
3750.734 0.738 0.736 0.580
187.50.415 0.436 0.426 0.270
940.311 0.328 0.320 0.164
470.231 0.245 0.238 0.082
23.50.198 0.189 0.194 0.038
00.155 0.157 0.156
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Chemotactic factor for t lymphocytes but not monocytes or granulocytes. May play a role in T-cell development in thymus and in trafficking and activation of mature T-cells. Binds to CCR4 and CCR8 (By similarity).
Gene References into Functions
  1. The present results suggest that TNF-alpha-induced CCL17 mRNA transcription in canine keratinocytes is positively regulated by p38 but negatively controlled by ERK. PMID: 20837364
  2. This study suggests that interferon-gamma may suppress granulocyte-macrophage colony stimulating factor production from canine keratinocytes, although further studies are required to confirm this. PMID: 20860556
  3. Inflammatory cytokines are important inducing factors for the production of CCL17 in the lesional skin of dogs with atopic dermatitis. atopic dermatitis. PMID: 20022349
Subcellular Location Secreted
Protein Families Intercrine beta (chemokine CC) family
Tissue Specificity Expressed in thymus, spleen, lymph node, lung and heart.
Database Links

KEGG: cfa:403586

STRING: 9615.ENSCAFP00000012872

UniGene: Cfa.3450

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