Chicken Interleukin 6,IL-6 ELISA Kit

Code CSB-E08549Ch
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Chicken IL6 ELISA Kit was designed for the quantitative measurement of Chicken IL6 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL .

Target Name interleukin 6 (interferon, beta 2)
Alternative Names IL6 ELISA Kit; Interleukin-6 ELISA Kit; ChIL-6 ELISA Kit; IL-6 ELISA Kit
Abbreviation IL6
Uniprot No. Q90YI0
Species Gallus gallus (Chicken)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 93  
Range % 85-97  
1:2 Average % 92  
Range % 85-99  
1:4 Average % 95  
Range % 89-100  
1:8 Average % 92  
Range % 85-100  
Recovery
The recovery of chicken IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 98 91-105  
EDTA plasma (n=4) 96 88-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1000 2.487 2.570 2.529 2.454  
500 2.204 2.230 2.217 2.142  
250 1.800 1.764 1.782 1.707  
125 1.390 1.394 1.392 1.317  
62.5 0.917 0.950 0.934 0.859  
31.2 0.584 0.568 0.576 0.501  
15.6 0.348 0.362 0.355 0.280  
0 0.074 0.075 0.075    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

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Target Background

Function
(From Uniprot)
Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway. The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells.
Gene References into Functions
  1. Avian leukosis virus subgroup J induces VEGF expression via NF-kappaB/PI3K-dependent IL-6 production to promoter tumorigenesis. PMID: 27852059
  2. The effects of daily corticosterone injections on acute phase proteins (APP), IL-6, and HSP70 are reported; APP and HSP70 may be of value as indicators of stress in poultry. PMID: 25306460
  3. Chicken IL-6 is a heat-shock gene PMID: 24055475
  4. Nampt/visfatin/PBEF affects expression of myogenic regulatory factors and is regulated by interleukin-6 in chicken skeletal muscle cells PMID: 21545843
  5. IL-6 is involved in the progression of chicken arthritis induced by Staph. aureus, and it contributes to disease incidence and mortality PMID: 17495100

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Subcellular Location Secreted.
Protein Families IL-6 superfamily
Database Links

KEGG: gga:395337

UniGene: Gga.2769

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