Chicken Interleukin 6,IL-6 ELISA Kit

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Chicken Interleukin 6,IL-6 ELISA Kit

Citations (21) Protocol

Code	CSB-E08549Ch
Size	96T,5×96T,10×96T How to order?
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Product Details
Citations (21)
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Description

This Chicken IL6 ELISA Kit was designed for the quantitative measurement of Chicken IL6 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL .

Target Name

interleukin 6 (interferon, beta 2)

Alternative Names

IL6 ELISA Kit; Interleukin-6 ELISA Kit; ChIL-6 ELISA Kit; IL-6 ELISA Kit

Abbreviation

IL6

Uniprot No.

Q90YI0

Species

Gallus gallus (Chicken)

Sample Types

serum, plasma, tissue homogenates

Detection Range

15.6 pg/mL-1000 pg/mL

Sensitivity

3.9 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	85-97
1:2	Average %	92
	Range %	85-99
1:4	Average %	95
	Range %	89-100
1:8	Average %	92
	Range %	85-100

Recovery

The recovery of chicken IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	91-105
EDTA plasma (n=4)	96	88-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.487	2.570	2.529	2.454
500	2.204	2.230	2.217	2.142
250	1.800	1.764	1.782	1.707
125	1.390	1.394	1.392	1.317
62.5	0.917	0.950	0.934	0.859
31.2	0.584	0.568	0.576	0.501
15.6	0.348	0.362	0.355	0.280
0	0.074	0.075	0.075

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Synergistic Immunosuppression of Avian Leukosis Virus Subgroup J and Infectious Bursal Disease Virus Is Responsible for Enhanced Pathogenicity W Chen,Viruses,2022
Dual-Drug Loaded Separable Microneedles for Efficient Rheumatoid Arthritis Therapy M An,Pharmaceutics,2022
Hydroxy-Selenomethionine Improves the Selenium Status and Helps to Maintain Broiler Performances under a High Stocking Density and Heat Stress Conditions through a Better Redox and Immune Response H Sun,Antioxidants,2021
Comparative evaluation of therapeutic efficacy of sulfadiazine-trimethoprim, oxytetracycline, enrofloxacin and florfenicol on Staphylococcus aureus-induced arthritis in broilers AM BOROOJENI,Randomized Controlled Trial,2021
Clostridium butyricum alone or combined with 1, 25‐dihydroxyvitamin D3 improved early‐stage broiler health by modulating intestinal flora Y Yu,Journal of Applied Microbiology,2021
Influence of Dietary Supplementation of Kigelia pinnata and Plukenetia conophora Leaves on Cytokine Expression, Immunoglobulins, Blood Chemistry, Caecal Microbiota and Meat Quality in Broiler Chickens KD Adeyemi,Poultry Science Journal,2021
Dietary encapsulated Bacillus subtilis and essential oil supplementation improves reproductive performance and hormone concentrations of broiler breeders during the late laying period Y.B. Chen,Livestock Science,2021
Effects of flaxseed supplementation on omega-6 to omega-3 fatty acid balance, lipid mediator profile, proinflammatory cytokines and stress indices in laying hens SM Le,researchsquare,2020
Enhanced cytokine expression and upregulation of inflammatory signaling pathways in broiler chickens affected by wooden breast myopathy T Xing,J. Sci. Food Agric,2020
Egg White/Egg Shell based Biomimetic Hybrid Hydrogels for Bone Regeneration Keqing Huang, et al,Acs Biomaterials-Science & Engineering,2019
Insulin resistance and metabonomics analysis of fatty liver haemorrhagic syndrome in laying hens induced by a high-energy low-protein diet Zhuang Y, et al,Scientific Reports,2019
Selenium Deficiency Aggravates Aflatoxin B1-Induced Immunotoxicity in Chick Spleen by Regulating 6 Selenoprotein Genes and Redox/Inflammation/Apoptotic Signaling Zhao L,The Journal of Nutrition,2019
Grape Seed Proanthocyanidin Extract Alleviates AflatoxinB1-Induced Immunotoxicity and Oxidative Stress via Modulation of NF-κB and Nrf2 Signaling Pathways in Broilers Shahid Ali Rajput, et al,Toxins,2019
Effects of pre-hatch thermal manipulation and post-hatch acute heat stress on the mRNA expression of interleukin-6 and genes involved in its induction pathways in 2 broiler chicken breeds Al-Zghoul MB, et al,Poultry Science,2018
Dietary Supplementation of Purified Amino Acid Derived from Animal Blood on Immune Response and Growth Performance of Broiler Chicken T. G. Wandita.et al,Tropical Animal Science Journal,2018
Immunization with avian metapneumovirus harboring chicken Fc induces higher immune responses. Paudel S.et al,Virus Res.,2016
Comparative evaluation of therapeutic efficacy of sulfadiazine-trimethoprim, oxytetracycline, enrofloxacin and florfenicol on Staphylococcus aureus-induced arthritis in broilers N. Mosleh.et al,Publishing models and article dates explained,2016
Comparative evaluation of therapeutic efficacy of sulfadiazine-trimethoprim, oxytetracycline, enrofloxacin and florfenicol on Staphylococcus aureus - induced arthritis in broilers N. Mosleh .et al,British Poultry Science,2016
Visfatin and ghrelin: can they be forthcoming biomarkers or new drug targets for asthma? Toru Uuml. et al,Int J Clin Exp Med.,2015
The antimicrobial peptide sublancin ameliorates necrotic enteritis induced by in broilers S Wang. et al,Journal of Animal Science,2015
Immune responses to oral vaccination with Salmonella-delivered avian pathogenic Escherichia coli antigens and protective efficacy against colibacillosis. Lee JH.et al,Can J Vet Res.,2015

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Function (From Uniprot)	Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway. The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells.
Gene References into Functions	Avian leukosis virus subgroup J induces VEGF expression via NF-kappaB/PI3K-dependent IL-6 production to promoter tumorigenesis. PMID: 27852059 The effects of daily corticosterone injections on acute phase proteins (APP), IL-6, and HSP70 are reported; APP and HSP70 may be of value as indicators of stress in poultry. PMID: 25306460 Chicken IL-6 is a heat-shock gene PMID: 24055475 Nampt/visfatin/PBEF affects expression of myogenic regulatory factors and is regulated by interleukin-6 in chicken skeletal muscle cells PMID: 21545843 IL-6 is involved in the progression of chicken arthritis induced by Staph. aureus, and it contributes to disease incidence and mortality PMID: 17495100 Show More Hide All
Subcellular Location	Secreted.
Protein Families	IL-6 superfamily
Database Links	KEGG: gga:395337 UniGene: Gga.2769

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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