Chicken Interleukin 6,IL-6 ELISA Kit

Code CSB-E08549Ch
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q90YI0
Lead Time 3-5 working days
Abbreviation IL6
Protein Biological Process 1 Immunity
Target Name interleukin 6 (interferon, beta 2)
Alias BSF2, HGF, HSF, IFNB2, IL-6, B cell stimulatory factor-2|B-cell differentiation factor|CTL differentiation factor|OTTHUMP00000158544|hybridoma growth factor|interleukin 6|interleukin BSF-2
Species Gallus gallus (Chicken)
Protein Biological Process 3 Acute phase
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes a cytokine that functions in inflammation and the maturation of B cells. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis.
HGNC 1834
RGD 2327
MGI 88373
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 93  
Range % 85-97  
1:2 Average % 92  
Range % 85-99  
1:4 Average % 95  
Range % 89-100  
1:8 Average % 92  
Range % 85-100  
Recovery
The recovery of chicken IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 98 91-105  
EDTA plasma (n=4) 96 88-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1000 2.487 2.570 2.529 2.454  
500 2.204 2.230 2.217 2.142  
250 1.800 1.764 1.782 1.707  
125 1.390 1.394 1.392 1.317  
62.5 0.917 0.950 0.934 0.859  
31.2 0.584 0.568 0.576 0.501  
15.6 0.348 0.362 0.355 0.280  
0 0.074 0.075 0.075    
References
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Function Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response (By similarity).
Subcellular Location Secreted
Protein Families IL-6 superfamily
Database Links

KEGG: gga:395337

UniGene: Gga.2769

Pathway HIF-1 signaling pathway
Jak-STAT signaling pathway
PI3K-Akt signaling pathway
TNF signaling pathway
Cellular senescence
Hematopoietic cell lineage
IL-17 signaling pathway
NOD-like receptor signaling pathway
Th17 cell differentiation
Toll-like receptor signaling pathway
AGE-RAGE signaling pathway in diabetic complications
FoxO signaling pathway
Inflammatory Pain

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