Chicken Interleukin 6,IL-6 ELISA Kit

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Chicken Interleukin 6,IL-6 ELISA Kit

Specification
Target

Code

CSB-E08549Ch

See More Details

Product Type

ELISA Kit

Size

96T,5×96T,10×96T

Uniprot No.

Q90YI0

Lead Time

3-5 working days

Abbreviation

IL6

Protein Biological Process 1

Immunity

Target Name

interleukin 6 (interferon, beta 2)

Alias

Species

Gallus gallus (Chicken)

Protein Biological Process 3

Acute phase

Sample Types

serum, plasma, tissue homogenates

Detection Range

15.6 pg/mL-1000 pg/mL

Sensitivity

3.9 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Protocol

Instructions

Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.

Assay Principle

quantitative

Measurement

Sandwich

Target Details

This gene encodes a cytokine that functions in inflammation and the maturation of B cells. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis.

HGNC

1834

RGD

2327

MGI

88373

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	85-97
1:2	Average %	92
	Range %	85-99
1:4	Average %	95
	Range %	89-100
1:8	Average %	92
	Range %	85-100

Recovery

The recovery of chicken IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	91-105
EDTA plasma (n=4)	96	88-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.487	2.570	2.529	2.454
500	2.204	2.230	2.217	2.142
250	1.800	1.764	1.782	1.707
125	1.390	1.394	1.392	1.317
62.5	0.917	0.950	0.934	0.859
31.2	0.584	0.568	0.576	0.501
15.6	0.348	0.362	0.355	0.280
0	0.074	0.075	0.075

References

Immune responses to oral vaccination with Salmonella-delivered avian pathogenic Escherichia coli antigens and protective efficacy against colibacillosis. Lee JH.et al,Can J Vet Res.,2015 79(3):229-34.
The antimicrobial peptide sublancin ameliorates necrotic enteritis induced by in broilers S Wang. et al,Journal of Animal Science,2015 Oct;93(10):4750-60
Visfatin and ghrelin: can they be forthcoming biomarkers or new drug targets for asthma? Toru Uuml. et al,Int J Clin Exp Med.,2015 Apr 15;8(4):6257-61. eCollection 2015 .
Comparative evaluation of therapeutic efficacy of sulfadiazine-trimethoprim, oxytetracycline, enrofloxacin and florfenicol on Staphylococcus aureus - induced arthritis in broilers N. Mosleh .et al,British Poultry Science,2016
Comparative evaluation of therapeutic efficacy of sulfadiazine-trimethoprim, oxytetracycline, enrofloxacin and florfenicol on Staphylococcus aureus-induced arthritis in broilers N. Mosleh.et al,Publishing models and article dates explained,2016
Immunization with avian metapneumovirus harboring chicken Fc induces higher immune responses. Paudel S.et al,Virus Res.,220:129-35
Dietary Supplementation of Purified Amino Acid Derived from Animal Blood on Immune Response and Growth Performance of Broiler Chicken T. G. Wandita.et al,Tropical Animal Science Journal,March 5
Effects of pre-hatch thermal manipulation and post-hatch acute heat stress on the mRNA expression of interleukin-6 and genes involved in its induction pathways in 2 broiler chicken breeds Al-Zghoul MB, et al,Poultry Science,Octobert 25
Grape Seed Proanthocyanidin Extract Alleviates AflatoxinB1-Induced Immunotoxicity and Oxidative Stress via Modulation of NF-κB and Nrf2 Signaling Pathways in Broilers Shahid Ali Rajput, et al,Toxins,January 7

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Function	Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response (By similarity).
Subcellular Location	Secreted
Protein Families	IL-6 superfamily
Database Links	KEGG: gga:395337 UniGene: Gga.2769
Pathway	HIF-1 signaling pathway Jak-STAT signaling pathway PI3K-Akt signaling pathway TNF signaling pathway Cellular senescence Hematopoietic cell lineage IL-17 signaling pathway NOD-like receptor signaling pathway Th17 cell differentiation Toll-like receptor signaling pathway AGE-RAGE signaling pathway in diabetic complications FoxO signaling pathway Inflammatory Pain

IL6 Protein

IL6 Proteins for Gallus gallus (Chicken)

Code	Product Name	Source
CSB-YP856206CH CSB-EP856206CH CSB-BP856206CH CSB-MP856206CH	Recombinant Chicken Interleukin-6(IL6)	Yeast E.coli Baculovirus Mammalian cell

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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