Chicken cortisol (COR) ELISA kit

Instructions
Code CSB-EQ027342CH
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name cortisol (COR)
Abbreviation COR
Species Gallus gallus (Chicken)
Sample Types serum, plasma
Detection Range 0.049 ng/mL-200 ng/mL
Sensitivity 0.049 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of chicken cortisol in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 84    
Range % 80-88    
1:2 Average % 92    
Range % 88-96    
1:4 Average % 106    
Range % 101-112    
1:8 Average % 95    
Range % 90-100    
Recovery
The recovery of chicken cortisol spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 97 92-101    
EDTA plasma (n=4) 88 84-92    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
ng/ml OD1 OD2 Average      
200 0.369 0.381 0.375      
50 0.673 0.694 0.684      
12.5 0.993 1.009 1.001      
3.125 1.566 1.617 1.592      
0.781 2.094 2.025 2.060      
0.195 2.396 2.308 2.352      
0.049 2.551 2.520 2.536      
0 2.898 2.832 2.865      
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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