Chicken cortisol (COR) ELISA kit

Code CSB-EQ027342CH
Size 96T,5×96T,10×96T
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now
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Product Details

Target Name
cortisol (COR)
Abbreviation
COR
Species
Gallus gallus (Chicken)
Sample Types
serum, plasma
Detection Range
0.049 ng/mL-200 ng/mL
Sensitivity
0.049 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of chicken cortisol in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 84    
Range % 80-88    
1:2 Average % 92    
Range % 88-96    
1:4 Average % 106    
Range % 101-112    
1:8 Average % 95    
Range % 90-100    
Recovery
The recovery of chicken cortisol spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 97 92-101    
EDTA plasma (n=4) 88 84-92    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
ng/ml OD1 OD2 Average      
200 0.369 0.381 0.375      
50 0.673 0.694 0.684      
12.5 0.993 1.009 1.001      
3.125 1.566 1.617 1.592      
0.781 2.094 2.025 2.060      
0.195 2.396 2.308 2.352      
0.049 2.551 2.520 2.536      
0 2.898 2.832 2.865      
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days
Description

This Chicken COR ELISA Kit was designed for the quantitative measurement of Chicken COR protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 0.049 ng/mL-200 ng/mL and the sensitivity is 0.049 ng/mL.

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