Dog C-C motif chemokine 5(CCL5) ELISA kit

Instructions
Code CSB-EL004800DO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name chemokine (C-C motif) ligand 5
Alternative Names CCL5 ELISA kit; SCYA5C-C motif chemokine 5 ELISA kit; Small-inducible cytokine A5 ELISA kit; T-cell-specific protein RANTES ELISA kit
Abbreviation CCL5
Uniprot No. Q8HYS0
Species Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.081 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of dog CCL5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 105
Range % 97-110
1:2 Average % 101
Range % 95-106
1:4 Average % 96
Range % 87-101
1:8 Average % 102
Range % 97-107
Recovery
The recovery of dog CCL5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 104 96-108
EDTA plasma (n=4) 99 91-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected CV
10 2.658 2.600 2.629 2.510 1.56%
5 1.985 2.016 2.001 1.882 1.10%
2.5 1.348 1.369 1.359 1.240 1.09%
1.25 0.919 0.927 0.923 0.804 0.61%
0.625 0.502 0.523 0.513 0.394 2.89%
0.313 0.294 0.314 0.304 0.185 4.65%
0.156 0.212 0.219 0.216 0.097 2.29%
0 0.117 0.121 0.119   2.38%
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Data

Function Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. May also be an agonist of the G protein-coupled receptor GPR75. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells.
Gene References into Functions
  1. Data indicate that the Leishmania braziliensis antigens plus saponin (LBSap) vaccine induced high levels of IL-12, IL-10, CCL4, CCL5 and CXCL8 expression within 48 hours. PMID: 23911411
  2. Adipose tissue-derived mesenchymal stem cells expressed the mRNA of transforming growth factor beta (TGF-beta), IL-6, IL-8, CCL2, CCL5, VEGF,HGF, tissue inhibitor metalloproteinase-1/2, and cyclooxygenase-2 but not that of IL-10. PMID: 18717642
Subcellular Location Secreted
Protein Families Intercrine beta (chemokine CC) family
Database Links

KEGG: cfa:403522

STRING: 9615.ENSCAFP00000026835

UniGene: Cfa.21

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