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Dog C-C motif chemokine 5(CCL5) ELISA kit

Dog C-C motif chemokine 5(CCL5) ELISA kit

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Code	CSB-EL004800DO
Size	96T,5×96T,10×96T
Trial Size
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Target Data

Product Details

Target Name

chemokine (C-C motif) ligand 5

Alternative Names

CCL5 ELISA kit; SCYA5C-C motif chemokine 5 ELISA kit; Small-inducible cytokine A5 ELISA kit; T-cell-specific protein RANTES ELISA kit

Abbreviation

CCL5

Uniprot No.

Q8HYS0

Species

Canis lupus familiaris (Dog) (Canis familiaris)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.156 ng/mL-10 ng/mL

Sensitivity

0.081 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of dog CCL5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	105
	Range %	97-110
1:2	Average %	101
	Range %	95-106
1:4	Average %	96
	Range %	87-101
1:8	Average %	102
	Range %	97-107

Recovery

The recovery of dog CCL5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	96-108
EDTA plasma (n=4)	99	91-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected	CV
10	2.658	2.600	2.629	2.510	1.56%
5	1.985	2.016	2.001	1.882	1.10%
2.5	1.348	1.369	1.359	1.240	1.09%
1.25	0.919	0.927	0.923	0.804	0.61%
0.625	0.502	0.523	0.513	0.394	2.89%
0.313	0.294	0.314	0.304	0.185	4.65%
0.156	0.212	0.219	0.216	0.097	2.29%
0	0.117	0.121	0.119		2.38%

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

5-7 working days

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Target Data

Function	Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. May also be an agonist of the G protein-coupled receptor GPR75. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells.
Gene References into Functions	Data indicate that the Leishmania braziliensis antigens plus saponin (LBSap) vaccine induced high levels of IL-12, IL-10, CCL4, CCL5 and CXCL8 expression within 48 hours. PMID: 23911411 Adipose tissue-derived mesenchymal stem cells expressed the mRNA of transforming growth factor beta (TGF-beta), IL-6, IL-8, CCL2, CCL5, VEGF,HGF, tissue inhibitor metalloproteinase-1/2, and cyclooxygenase-2 but not that of IL-10. PMID: 18717642
Subcellular Location	Secreted
Protein Families	Intercrine beta (chemokine CC) family
Database Links	KEGG: cfa:403522 STRING: 9615.ENSCAFP00000026835 UniGene: Cfa.21

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Related pathways

Chemokine signaling pathway TNF signaling pathway NOD-like receptor signaling pathway Toll-like receptor signaling pathway

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Dog C-C motif chemokine 5(CCL5) ELISA kit

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