Fish Tumor Necrosis Factor α(TNF-α) ELISA KIT

Product Details

Citations

Target Data

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Fish Tumor Necrosis Factor α(TNF-α) ELISA KIT

Name: Fish Tumor Necrosis Factor α(TNF-α) ELISA KIT
Brand: CUSABIO
SKU: CSB-E13254Fh

^Instructions

Code	CSB-E13254Fh
Size	96T,5×96T,10×96T
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Product Details
Citations
Target Data

Target Name

Tumor Necrosis Factor α(TNF-α)

Abbreviation

TNF-α

Uniprot No.

Q8JFG3

Species

Fish

Sample Types

serum, plasma, tissue homogenates

Detection Range

125 pg/mL-5000 pg/mL

Sensitivity

125 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of fish TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	89-100
1:2	Average %	93
	Range %	87-99
1:4	Average %	95
	Range %	88-103
1:8	Average %	96
	Range %	90-102

Recovery

The recovery of fish TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	95-102
EDTA plasma (n=4)	95	91-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
5000	0.154	0.156	0.155
2500	0.351	0.328	0.340
1150	0.562	0.535	0.549
375	0.861	0.864	0.863
125	1.544	1.530	1.537
0	2.387	2.355	2.371

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Chronic stress of high dietary carbohydrate level causes inflammation and influences glucose transport through SOCS3 in Japanese flounder Paralichthys olivaceus Kangyu Deng.et al,SCI REP-UK,2018
Cytokine Responses in Gills of Capoeta umbla as Biomarkers of Environmental Pollution Danabas D.et al,Water Environ Res.,2016
Immunomodulation of Lactobacillus pentosus PL11 against Edwardsiella tarda infection in the head kidney cells of the Japanese eel (Anguilla japonica). Birhanu BT.et al,Fish Shellfish Immunol.,2016

Function	Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR.
Subcellular Location	Membrane, Single-pass type II membrane protein
Protein Families	Tumor necrosis factor family

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

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