Fish Tumor Necrosis Factor α(TNF-α) ELISA KIT

Instructions
Code CSB-E13254Fh
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name Tumor Necrosis Factor α(TNF-α)
Abbreviation TNF-α
Uniprot No. Q8JFG3
Species Fish
Sample Types serum, plasma, tissue homogenates
Detection Range 125 pg/mL-5000 pg/mL
Sensitivity 125 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of fish TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 95  
Range % 89-100  
1:2 Average % 93  
Range % 87-99  
1:4 Average % 95  
Range % 88-103  
1:8 Average % 96  
Range % 90-102  
Recovery
The recovery of fish TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 99 95-102  
EDTA plasma (n=4) 95 91-101  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
5000 0.154 0.156 0.155    
2500 0.351 0.328 0.340    
1150 0.562 0.535 0.549    
375 0.861 0.864 0.863    
125 1.544 1.530 1.537    
0 2.387 2.355 2.371    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR.
Subcellular Location Membrane, Single-pass type II membrane protein
Protein Families Tumor necrosis factor family

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