Target Name |
Tumor Necrosis Factor α(TNF-α) |
Abbreviation |
TNF-α |
Uniprot No. |
Q8JFG3 |
Species |
Fish |
Sample Types |
serum, plasma, tissue homogenates |
Detection Range |
125 pg/mL-5000 pg/mL |
Sensitivity |
125 pg/mL |
Assay Time |
1-5h |
Sample Volume |
50-100ul |
Detection Wavelength |
450 nm |
Research Area |
Immunology
|
Assay Principle |
quantitative |
Measurement |
Competitive |
Precision |
Intra-assay Precision (Precision within an assay): CV%<15% |
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Three samples of known concentration were tested twenty times on one plate to assess. |
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Inter-assay Precision (Precision between assays): CV%<15% |
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Three samples of known concentration were tested in twenty assays to assess. |
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Linearity |
To assess the linearity of the assay, samples were spiked with high concentrations of fish TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
|
Sample |
Serum(n=4) |
|
1:1 |
Average % |
95 |
|
Range % |
89-100 |
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1:2 |
Average % |
93 |
|
Range % |
87-99 |
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1:4 |
Average % |
95 |
|
Range % |
88-103 |
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1:8 |
Average % |
96 |
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Range % |
90-102 |
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Recovery |
The recovery of fish TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
Sample Type |
Average % Recovery |
Range |
|
Serum (n=5) |
99 |
95-102 |
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EDTA plasma (n=4) |
95 |
91-101 |
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Typical Data |
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
pg/ml |
OD1 |
OD2 |
Average |
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5000 |
0.154 |
0.156 |
0.155 |
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2500 |
0.351 |
0.328 |
0.340 |
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1150 |
0.562 |
0.535 |
0.549 |
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375 |
0.861 |
0.864 |
0.863 |
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125 |
1.544 |
1.530 |
1.537 |
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0 |
2.387 |
2.355 |
2.371 |
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Troubleshooting
and FAQs |
ELISA kit FAQs |
Storage |
Store at 2-8°C. Please refer to protocol. |
Lead Time |
3-5 working days |