Guinea pig Interleukin 8,IL-8 ELISA Kit

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Guinea pig Interleukin 8,IL-8 ELISA Kit

Name: Guinea pig Interleukin 8,IL-8 ELISA Kit
Brand: CUSABIO
SKU: CSB-E13096Gp

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Code	CSB-E13096Gp
Size	96T,5×96T,10×96T
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Target Data

Target Name

interleukin 8

Alternative Names

CXCL8 ELISA Kit; IL8 ELISA Kit; Interleukin-8 ELISA Kit; IL-8 ELISA Kit; C-X-C motif chemokine 8 ELISA Kit; Chemokine ELISA Kit; C-X-C motif) ligand 8 ELISA Kit; Neutrophil attractant protein 1 ELISA Kit; NAP-1 ELISA Kit

Abbreviation

IL8

Uniprot No.

P49113

Species

Cavia porcellus (Guinea pig)

Sample Types

serum, plasma, tissue homogenates

Detection Range

31.25 pg/mL-2000 pg/mL

Sensitivity

7.8 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-102
1:2	Average %	95
	Range %	85-100
1:4	Average %	95
	Range %	88-102
1:8	Average %	97
	Range %	90-104

Recovery

The recovery of guinea pig IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	95-102
EDTA plasma (n=4)	94	90-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.253	2.245	2.249	2.134
1000	1.631	1.696	1.664	1.549
500	1.111	1.135	1.123	1.008
250	0.717	0.752	0.735	0.620
125	0.434	0.423	0.429	0.314
62.5	0.343	0.354	0.349	0.234
31.25	0.228	0.241	0.235	0.120
0	0.116	0.113	0.115

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Up-regulation of microRNA-335-5p Reduces Inflammation via Negative Regulation of the TPX2-mediated AKT/GSK3β Signaling Pathway in a Chronic Rhinosinusitis Mouse Model X Gu,Cellular Signalling,2020
Effect of folate supplementation on immunological and autophagy markers in experimental nonalcoholic fatty liver disease T Minato,European Cytokine Network volume,2019
Effects of early protein restriction on the growth performance and gut development of pigs fed diets with or without antibiotic Zhao X,animal,2019
Maternal dietary resveratrol alleviates weaning-associated diarrhea and intestinal inflammation in pig offspring by changing intestinal gene expression and microbiota Qingwei Meng, et al,Food & Function,2019
Mycoplasma hyopneumoniae Mhp597 is a Cytotoxicity, Inflammation and Immunosuppression Associated Nuclease PengLi,Veterinary Microbiology,2019
Arginase inhibition prevents inflammation and remodeling in a guinea pig model of COPD Pera T et al,J Pharmacol Exp Ther,2014

Function	IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus (By similarity).
Subcellular Location	Secreted
Protein Families	Intercrine alpha (chemokine CxC) family
Database Links	STRING: 10141.ENSCPOP00000009984

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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