Horse hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A) ELISA kit

Instructions
Code CSB-EL010351HO
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A)
Abbreviation HIF1A
Uniprot No. F6VG41
Species Equus caballus (Horse)
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.81 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Epigenetics and Nuclear Signaling
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of horse HIF1A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 89
Range % 85-94
1:2 Average % 102
Range % 96-107
1:4 Average % 92
Range % 87-98
1:8 Average % 94
Range % 89-98
Recovery
The recovery of horse HIF1A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 97 93-102
EDTA plasma (n=4) 103 92-107
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
2000 1.899 1.957 1.928 1.819
1000 1.417 1.496 1.457 1.348
500 0.926 0.978 0.952 0.843
250 0.583 0.572 0.578 0.469
125 0.345 0.353 0.349 0.240
62.5 0.246 0.252 0.249 0.140
31.25 0.171 0.178 0.175 0.066
0 0.108 0.109 0.109  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
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