Human C-X-C motif chemokine 3(CXCL3) ELISA kit

Code CSB-EL006249HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Human CXCL3 ELISA Kit was designed for the quantitative measurement of Human CXCL3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 9.37 pg/mL-600 pg/mL and the sensitivity is 2.34 pg/mL .

Target Name chemokine (C-X-C motif) ligand 3
Alternative Names C-X-C motif chemokine 3 ELISA Kit; C-X-C motif chemokine ligand 3 ELISA Kit; Chemokine (C X C motif) ligand 3 ELISA Kit; Chemokine (CXC motif) ligand 3 ELISA Kit; Cinc 2 ELISA Kit; CINC 2b ELISA Kit; Cinc2 ELISA Kit; CINC2b ELISA Kit; CXCL 3 ELISA Kit; Cxcl3 ELISA Kit; CXCL3_HUMAN ELISA Kit; Cytokine induced neutrophil chemoattractant 2 ELISA Kit; Dcip1 ELISA Kit; Dendritic cell inflammatory protein 1 ELISA Kit; Gm1960 ELISA Kit; GRO protein gamma ELISA Kit; GRO-gamma ELISA Kit; GRO-gamma(1-73) ELISA Kit; GRO-gamma(5-73) ELISA Kit; GRO3 ELISA Kit; GRO3 oncogene ELISA Kit; GROG ELISA Kit; Growth regulated protein gamma ELISA Kit; Growth-regulated protein gamma ELISA Kit; Macrophage inflammatory protein 2 beta precursor ELISA Kit; Macrophage inflammatory protein 2-beta ELISA Kit; Melanoma growth stimulatory activity gamma ELISA Kit; Member 3 ELISA Kit; MGSA gamma ELISA Kit; MIP 2b ELISA Kit; MIP2-beta ELISA Kit; MIP2B ELISA Kit; SCYB3 ELISA Kit; Small inducible cytokine subfamily B ELISA Kit
Abbreviation CXCL3
Uniprot No. P19876
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 9.37 pg/mL-600 pg/mL
Sensitivity 2.34 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CXCL3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %90
Range %87-93
1:2Average %100
Range %97-103
1:4Average %95
Range %92-97
1:8Average %99
Range %95-102
Recovery
The recovery of human CXCL3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9491-97
EDTA plasma (n=4)9793-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
6002.943 2.888 2.916 2.764
3002.188 2.299 2.244 2.092
1501.458 1.392 1.425 1.273
750.778 0.790 0.784 0.632
37.50.392 0.382 0.387 0.235
18.750.288 0.269 0.279 0.127
9.370.240 0.234 0.237 0.085
00.153 0.151 0.152  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Ligand for CXCR2. Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. In vitro, the processed form GRO-gamma(5-73) shows a fivefold higher chemotactic activity for neutrophilic granulocytes.
Gene References into Functions
  1. CXCL3 exerts its carcinogenic potential by directly and/or indirectly regulating the downstream signaling pathways and the expression of transcription factors in PCa. PMID: 29524043
  2. Exogenous CXCL3 induced Erk1/2 and ETS1 phosphorylation and promoted CD133 expression. PMID: 27255419
  3. Our findings suggest that CXCL3 and its receptor CXCR2 are overexpressed in prostate cancer cells, prostate epithelial cells and prostate cancer tissues, which may play multiple roles in prostate cancer progression and metastasis. PMID: 26837773
  4. results support a functional role of CXCL3 in breast cancer metastasis and as a viable target for cancer therapy PMID: 24605943
  5. CXCL3 displays antimicrobial activity against E. coli and S. aureus. PMID: 12949249
  6. secreted growth-regulated oncogene chemokines, specifically GRO-gamma, in human Mesenchymal stromal cell-conditioned media have an effect on the differentiation and the function of human monocyte-derived dendritic cells. PMID: 23589610
  7. Data show that mesenchymal stem cells (MSCs) directly regulate T cell proliferation by induction of CXCL3 chemokine and its receptor, CXCR2 on the surface in T cells. PMID: 23023221
  8. demonstrates, for the first time, that BIRC3 (anti-apoptotic protein), COL3A1 (matrix protein synthesis), and CXCL3 (chemokine) were up-regulated in the thrombin-stimulated human umbilical vein endothelial cells PMID: 16356540
  9. GRO-gamma is a promising candidate for Th2-associated glomerular permeability factor in minimal change disease. PMID: 17389786
  10. Inhibition of ERK phosphorylation decreased expression of GRO3. PMID: 17466952
  11. Report gonadotropin-releasing hormone-regulated CXCL3 expression in human placentation. PMID: 19369450
  12. propose that chemokines belonging to the CXC family could play an important role in the etiology of tendon xanthoma (TX), with CXCL3 being a possible biological marker of onset and development of TX PMID: 19448742
  13. overexpression of CXCL13 in the intestine during inflammatory conditions favors mobilization of B cells and of LTi and NK cells with immunomodulatory and reparative functions. PMID: 19741597

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Subcellular Location Secreted.
Protein Families Intercrine alpha (chemokine CxC) family
Database Links

HGNC: 4604

OMIM: 139111

KEGG: hsa:2921

STRING: 9606.ENSP00000296026

UniGene: Hs.89690

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