Human Fibronectin,FN ELISA Kit

Code CSB-E04551h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P02751
Lead Time 3-5 working days
Abbreviation FN1
Protein Biological Process 1 Immunity
Target Name fibronectin 1
Alias CIG, DKFZp686F10164, DKFZp686H0342, DKFZp686I1370, DKFZp686O13149, ED-B, FINC, FN, FNZ, GFND, GFND2, LETS, MSF, OTTHUMP00000206762|OTTHUMP00000206767|cold-insoluble globulin|migration-stimulating fa
Species Homo sapiens (Human)
Protein Biological Process 3 Acute phase
Sample Types serum, plasma, tissue homogenates, cell culture supernates, cell lysates
Detection Range 12.5 ng/mL-800 ng/mL
Sensitivity 3.12 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined.
HGNC 3778
RGD 2624
MGI 95566
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human FN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:100Average %97
Range %92-104
1:200Average %95
Range %88-100
1:400Average %96
Range %89-103
1:800Average %93
Range %89-100
Recovery
The recovery of human FN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9589-99
EDTA plasma (n=4)9690-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
8002.119 2.126 2.123 2.010
4001.797 1.704 1.751 1.638
2001.274 1.285 1.280 1.167
1000.758 0.761 0.760 0.647
500.482 0.495 0.489 0.376
250.286 0.296 0.291 0.178
12.50.196 0.203 0.200 0.087
00.112 0.113 0.113  
References
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Function Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.; FUNCTION
Involvement in disease Glomerulopathy with fibronectin deposits 2 (GFND2)
Subcellular Location Secreted, extracellular space, extracellular matrix
Tissue Specificity Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are f
Database Links

HGNC: 3778

OMIM: 135600

KEGG: hsa:2335

UniGene: Hs.203717

Pathway PI3K-Akt signaling pathway
Focal adhesion
Regulation of actin cytoskeleton
AGE-RAGE signaling pathway in diabetic complications

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