Human Interleukin 8,IL-8 ELISA KIT

Instructions
Code CSB-E04641h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name interleukin 8
Alternative Names CXCL8, GCP-1, GCP1, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1, T cell chemotactic factor|beta-thromboglobulin-like protein|chemokine (C-X-C motif) ligand 8|emoctakin|granulocyte chemotactic
Abbreviation IL8
Uniprot No. P10145
Species Homo sapiens (Human)
Sample Types serum, cell culture supernates, saliva, urine, cerebrospinal fluid (CSF), tissue homogenates, cell lysates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.110 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision

 Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess. 

Linearity

 To assess the linearity of the assay, samples were spiked with high concentrations of human IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Typical Data

 These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Citations

Q&A and Customer Reviews

 Customer Reviews
  • Sample type: Cell culture supernatant
    Sample species: Human
    Review: The mRNA level expressions of pro-inflammatory cytokines, IL-1α, IL-6, IL-8 and TNF-α were all assessed by qRT-PCR. * P<0.05, ** P<0.01, ***P<0.001 compared with the indicated group.
    PMID: 30026098
  • Sample type:Plasma
    Sample species: Human
    Review: Interleukin. Data are presented as the mean ± SD or n (%). Analysis performed using t-test or Student’s t-test.
    PMID: 30008611
  • Sample type: Cell culture supernatant
    Sample species: Human
    Review: IL-8 in the conditioned medium has no significant differences among these groups.
    PMID: 29755530
  • Sample type: Cell culture supernatant
    Sample species: Human
    Review: Serum Effect of various molecular weights of HA (30, 300 and 1300 kDa) on P.gingivalis-stimulated cytokine expression in HGFs. IL-8 production were determined by ELISA. Each value represents the mean ± S.D. from three separate experiments.
    PMID: 30465936

Target Data

Function IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
Subcellular Location Secreted
Protein Families Intercrine alpha (chemokine CxC) family
Database Links

HGNC: 6025

OMIM: 146930

KEGG: hsa:3576

STRING: 9606.ENSP00000306512

UniGene: Hs.624

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.