Human Interleukin 8,IL-8 ELISA KIT

Instructions
Code CSB-E04641h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

The Human Interleukin 8 (IL-8) ELISA Kit is engineered for accurate measurement of IL-8 levels from samples including serum, cell culture supernates, saliva, urine, cerebrospinal fluid (CSF), tissue homogenates, and cell lysates. It uses the Sandwich-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the IL-8 content in the sample. IL-8, also known as CXCL8, is a multi-functional pro-inflammatory chemokine. It exerts its biological effects by binding to the receptors CXCR1/IL-8R-A and CXCR2, eliciting a wide range of signaling pathways including the PI3K-Akt pathways. Studies showed that IL-8 could contribute to host defense but also to inflammatory diseases and neutrophil-mediated tissue damage. IL-8 has angiogenic activity on endothelial cells as well.

The kit has been validated against standards of sensitivity, specificity, precision, linearity, recovery, and lot-to-lot consistency. See more details through the product instructions.

Target Name interleukin 8
Alternative Names (Ala-IL-8)77 ELISA Kit; (Ser-IL-8)72 ELISA Kit; 9E3 ELISA Kit; Beta thromboglobulin like protein ELISA Kit; C-X-C motif chemokine 8 ELISA Kit; CEF-4 ELISA Kit; chemokine; CXC motif; ligand 8 ELISA Kit; CXCL8 ELISA Kit; Emoctakin ELISA Kit; GCP-1 ELISA Kit; GCP/IL-8 protein I ELISA Kit; GCP/IL-8 protein II ELISA Kit; GCP/IL-8 protein III ELISA Kit; GCP/IL-8 protein IV ELISA Kit; GCP/IL-8 protein V ELISA Kit; GCP/IL-8 protein VI ELISA Kit; GCP1 ELISA Kit; Granulocyte chemotactic protein 1 ELISA Kit; IL-8 ELISA Kit; IL-8(1-77) ELISA Kit; IL-8(9-77) ELISA Kit; IL8 ELISA Kit; IL8/NAP1 form I ELISA Kit; IL8/NAP1 form II ELISA Kit; IL8/NAP1 form III ELISA Kit; IL8/NAP1 form IV ELISA Kit; IL8/NAP1 form V ELISA Kit; IL8/NAP1 form VI ELISA Kit; IL8_HUMAN ELISA Kit; Inteleukin 8 ELISA Kit; LECT ELISA Kit; LUCT ELISA Kit; Lymphocyte-derived neutrophil-activating factor ELISA Kit; LYNAP ELISA Kit; MDNCF ELISA Kit; MDNCF-b ELISA Kit; MDNCF-c ELISA Kit; MONAP ELISA Kit; Monocyte derived neutrophil activating peptide ELISA Kit; Monocyte derived neutrophil chemotactic factor ELISA Kit; Monocyte-derived neutrophil chemotactic factor ELISA Kit; Monocyte-derived neutrophil-activating peptide ELISA Kit; NAF ELISA Kit; NAP 1 ELISA Kit; NAP-1 ELISA Kit; NAP1 ELISA Kit; Neutrophil activating peptide 1 ELISA Kit; Neutrophil activating protein 1 ELISA Kit; Neutrophil-activating factor ELISA Kit; Neutrophil-activating protein 1 ELISA Kit; Protein 3 10C ELISA Kit; Protein 3-10C ELISA Kit; SCYB 8 ELISA Kit; SCYB8 ELISA Kit; Small inducible cytokine subfamily B member 8 ELISA Kit; T cell chemotactic factor ELISA Kit; T-cell chemotactic factor ELISA Kit; TSG 1 ELISA Kit; TSG1 ELISA Kit
Abbreviation IL8
Uniprot No. P10145
Species Homo sapiens (Human)
Sample Types serum, cell culture supernates, saliva, urine, cerebrospinal fluid (CSF), tissue homogenates, cell lysates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.110 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision

 Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess. 

Linearity

 To assess the linearity of the assay, samples were spiked with high concentrations of human IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Typical Data

 These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Materials provided
  • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human IL-8 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
  • Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
  • One vial Biotin-labeled IL-8 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
  • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
  • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the high concentration Biotin-antibody to an appropriate working solution.
  • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the high concentration HRP-avidin solution to an appropriate solution.
  • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
  • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
  • One vial TMB Substrate (10 ml/bottle) --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
  • One vial Stop Solution (10 ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
  • An instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
  • An incubator that can provide stable incubation conditions up to 37°C±5°C.
  • Squirt bottle, manifold dispenser, or automated microplate washer.
  • Absorbent paper for blotting the microtiter plate.
  • 100ml and 500ml graduated cylinders.
  • Deionized or distilled water.
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL and pipette tips.
  • Timer
  • Test tubes for dilution.
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Customer Reviews and Q&A

 Customer Reviews
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4.7 - 3 reviews

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Sample type: Cell culture supernatant

Sample species: Human

Review: The mRNA level expressions of pro-inflammatory cytokines, IL-1α, IL-6, IL-8, and TNF-α were all assessed by qRT-PCR. * P<0.05, ** P<0.01, ***P<0.001 compared with the indicated group.

By Anonymous

Sample type: Cell culture supernatant

Sample species: Human

Review: IL-8 in the conditioned medium has no significant differences among these groups.

By Anonymous

Sample type: Cell culture supernatant

Sample species: Human

Review: Serum Effect of various molecular weights of HA (30, 300, and 1300 kDa) on P.gingivalis-stimulated cytokine expression in HGFs. IL-8 production was determined by ELISA. Each value represents the mean ± S.D. from three separate experiments.

By Anonymous

Target Background

Function
(From Uniprot)
IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
Gene References into Functions
  1. VEGF and IL-8 play a prominent role in the pathogenesis of early forms of rosacea and the hemostasis system. PMID: 29578433
  2. High levels of LIFR in colorectal cancer (CRC) facilitated proliferation and migration of endothelial cells, resulting in an increase in angiogenic activity. Moreover, IL-8 was found to play a role in the LIFR induced angiogenesis. IL-8 levels were correlated with LIFR levels in CRC tissues, whereas depletion of IL-8 led to a reduced angiogenic activity of LIFR in CRC cells. PMID: 29751081
  3. PKC-delta isoform plays a crucial role in Tat-TLR4 signaling pathway to activate NF-kappaB and CXCL8 production. PMID: 28539656
  4. CXCL8 was a target of miR-204, and miR-204 suppression could not increase cell viability, migration, invasion, and EMT procedure when CXCL8 was silenced. PMID: 29402343
  5. Interleukin 8 - 845 T/C and + 781 C/T polymorphisms were analyzed. For + 781C/T locus, in the dominant genetic model, significant difference between TT vs. CC + CT genotypes that significantly had a protective role against periodontitis disease. Positive association between distribution of IL8 - 845 T/C alleles and risk of periodontitis disease. C allele of IL-8 - 845 increased the risk of periodontitis disease. PMID: 30078118
  6. These results suggest a direct involvement of IL-8-CXCR1/2 axes in GBM progression by promoting both cell proliferation and invasion and indirectly by promoting neovascularization in the form of vascular mimicry. PMID: 30086759
  7. These results show that AKIP1 is crucial in cervical cancer angiogenesis and growth by elevating the levels of the NF-kappaB-dependent chemokines CXCL1, CXCL2, and CXCL8. PMID: 29520695
  8. The extramembranous domain of HofQ (emHofQ) was shown to interact with various cytokines, of which IL-8 exhibited the strongest interaction. PMID: 30088437
  9. he protein expression levels of IL-8 were significantly decreased in SZ patients, but no significant difference in the mRNA levels of IL-8 was observed between SZ patients and NC subjects. PMID: 28476335
  10. immune system process is indispensable in the progression of disease in colon, and identifies that IL-8 and MMP-9 play potential critical roles for the progression. PMID: 30074183
  11. IL-8 production was significantly enhanced following treatment with both IL-17A and CSE, while treatment with either IL-17A or CSE alone caused only a slight increase in IL-8 production. PMID: 29463070
  12. Work identified IL-8 as a positive regulator of homotypic CIC formation via enhancing intercellular adhesion. PMID: 30021676
  13. V2O5 induction of CXCL8 and CXCL11 chemokines may lead to the appearance and perpetuation of an inflammatory reaction into the dermal tissue. Further studies are required to evaluate dermal integrity and manifestations in subjects occupationally exposed, or living in polluted areas. PMID: 29901202
  14. Study findings point out that PAR2 could play an essential role in gastroesophageal reflux disease (GERD) pathogenesis - even repeated short-term exposure to weakly acidic conditions lead to the upregulation of PAR2 and subsequent activation of the intense IL-8 release in the esophageal mucosa and initiation of mucosal immune response in GERD. PMID: 29672302
  15. Given that IL-8, MIP-1beta, and MCP-1 are chemokines that play important roles in recruitment of immunocompetent cells for immune defense and tumor cell clearance, the observed lower levels of these markers with increasing PM2.5 exposure may provide insight into the mechanism by which DEE promotes lung cancer. PMID: 29023999
  16. These results suggested that stemness induction in SKOV3 cells by macrophages co-cultured with SKOV3-derived OCSLCs involved IL-8/STAT3 signaling. PMID: 29656182
  17. IL-8-251T>A (rs4073) Polymorphism is associated with gastric Cancer. PMID: 30275190
  18. expression level of CXCL8 had a positive relationship with recurrence probability in Acute myeloid leukemia. PMID: 29596823
  19. These data were in close agreement with the reduced cell migration and colony formation. Results from the present study suggested that reparixin and SCH527123 may be promising therapeutic agents for the treatment of pancreatic cancer by inhibiting the IL8/CXCR1/2 signaling cascade. PMID: 29749433
  20. A urinary IL-8 level of less than 61.25 pg/ml is more sensitive for prediction of complete remission in idiopathic membranous nephropathy patients. PMID: 29415357
  21. berberine inhibited the expression of MCP-1 and IL-8 induced by LPS. PMID: 28852897
  22. Regarding the IL-8 promoter T - 251A, the TA and AA genotypes were associated with significantly decreased risks of nasopharyngeal carcinoma (NPC) in a Taiwanese population compared with the wild-type TT genotype. The mRNA and protein expression levels for NPC tissues revealed no significant associations among the 20 NPC samples with different genotypes. PMID: 30200105
  23. IL-8 +781 T/C polymorphism is associated with the severe Clostridium difficile infection PMID: 29203364
  24. ShRNA mediated down-regulation of CXCL8 resulted in inhibition of cell proliferation, viability and invasion in vitro and a near complete growth reduction of tumor in vivo. PMID: 29679563
  25. CSF IL-8 concentrations were significantly elevated in CNS tumor patients as compared to non-tumoral individuals. AUC for CSF IL-8 was higher than for its index (CSF IL-8/serum IL-8). PMID: 29086194
  26. High IL8 expression is associated with melanoma. PMID: 29286146
  27. Lipo-CPFX, but not CPFX, retained the anti-IL-8 releasing activity. PMID: 29337216
  28. The results indicate significant contribution of IL8 on survival of hormonal dependent early-stage breast cancer patients and association with established parameters such as estrogen receptors/progesterone receptor and HER2. PMID: 28569250
  29. the frequency of non-classical monocytes expressing CXCL8 was increased in systemic sclerosis patient and monocytes expressing CXCL8 PMID: 29127442
  30. ntermediate Molecular Mass Hyaluronan and CD44 interactions enhanced normal PMN phagocytosis and IL-8 production PMID: 28730511
  31. serum levels in active vitiligo significantly elevated compared to those in stable vitiligo patients PMID: 29115683
  32. High IL8 expression is associated with pancreatic adenocarcinoma. PMID: 29205349
  33. Compared with controls, the interleukin (IL)-8 A/A genotype was more common in acute pancreatitis (AP). PMID: 29215544
  34. The presence of neither the first transmembrane helix of the receptor nor the lipid bilayer significantly affected the interactions of IL-8 with Binding Site-I of CXCR1. PMID: 29143165
  35. CXCL8 is highly expressed in cervical cancer tissues and cell lines, and correlated with malignant status and prognosis in cervical cancer patients. PMID: 28883082
  36. In conclusion, to our knowledge, this is the first study in the association of rs4073 and rs2227306 polymorphisms with childhood asthma risk in the Tunisian population. PMID: 28993876
  37. Results show that IL8 expression level is regulated by APE1 which activates NF-KB. PMID: 27388124
  38. aberrant miR-520c-3p expression may lead to reduced IL-8 expression and promote the mesenchymal phenotype in breast cancer cells, thereby increasing invasive growth. PMID: 29048659
  39. increased levels of IL-8 are associated with factors of worse prognosis in ovarian cancer PMID: 28872976
  40. Significantly elevated blood levels of IL-8 in myelodysplastic syndrome patients. PMID: 28856536
  41. the elevated concentrations of CXCL13, CXCL8, and CXCL10 or their increasing CSF/serum ratios may be potential biomarkers of neurosyphilis PMID: 27650493
  42. Results implicated the important role of PRL-3 in glycolysis metabolism through improving IL-8 secretion in colorectal cancer cells, and PRL-3 mediated glycolysis contributed to the promotion of cancer metastasis. PMID: 28791350
  43. Changes in serum IL-8 levels could be used to monitor and predict clinical benefit from immune checkpoint blockade in melanoma and NSCLC patients PMID: 28595336
  44. Lung cancer patients showed significantly lower levels of serum VEGF (1.9 fold) and IL-8 (~9 fold) than COPD patients. VEGF level was significantly higher (2.6 fold) in metastatic than non-metastatic cancer patients. An increase in MMP-9 (~1.6 fold) levels was observed in lung cancer patients. PMID: 27811960
  45. CXCL1/8 secreted by adipose-derived mesenchymal stem cells could promote breast cancer angiogenesis. PMID: 28514506
  46. our meta-analysis suggests that the IL-8 rs4073, A2767T, T11722T2, rs2234671, rs2230054, rs1126579, rs2227306, rs2227307, rs2227532, and T-738A polymorphisms are not associated with periodontitis while the IL-8 C1633T and rs1126580 polymorphisms may elevate the susceptibility of periodontitis based on the currently available evidences. PMID: 28446725
  47. An analogue of human CXCL8, CXCL8(3-72)K11R/G31P (hG31P) has been developed. PMID: 28754019
  48. inflammation triggered property of Microcystin-LR via IL-8/CXCR2 signaling PMID: 29197248
  49. A high IL-8 content in urine sampled on day 1 after renal transplantation was positively correlated with the activity of metalloproteinase-9 in urine. This proves that both of these chemokines cooperate in ischaemia-reperfusion injuries in transplanted kidneys. PMID: 28494217
  50. We discovered that IL-8 secreted from decidual stromal cells is a key cytokine enhancing the invasiveness of trophoblasts PMID: 28328096

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Subcellular Location Secreted
Protein Families Intercrine alpha (chemokine CxC) family
Database Links

HGNC: 6025

OMIM: 146930

KEGG: hsa:3576

STRING: 9606.ENSP00000306512

UniGene: Hs.624

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