24T ELISA Kit Trial Size (Only USD$150/ kit)
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Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of human TGF-β1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
The product CSB-E04725h is a ready-to-use microwave, strip plate ELISA kit designed to quantitatively analyze the TGFB1 in different biological samples, including serum, plasma, and cell culture supernates. This TGFB1 ELISA kit specifically recognizes the human TGFB1 in the sample. It adopts the Sandwich ELISA technique in conjunction with an enzyme colorimetric reaction to indicate the presence of human TGFB1 and quantitatively measures the level of TGFB1 in the samples. The intensity of the colored product is positively related to the amount of human TGFB1 in the sample.
Human TGFB1 is the most abundant TGFB isoform and was cloned from the human placenta. It is a pleiotropic growth factor that exerts multiple effects on inflammation, angiogenesis, fibrosis, tissue repair, and tumor progression. TGFB1 also exhibits various anti-inflammatory activities and contributes to immune homeostasis and inhibition of autoimmunity. TGFB1 acting as a very potent stimulator of chemotaxis stimulates monocyte, lymphocyte, neutrophil, and fibroblast migration. Dysregulated TGFB1 signaling has been associated with cellular proliferation, cancer development, and metastasis. TGFB1 upregulation mediated promotion of the epithelial-mesenchymal transition (EMT) enhances invasiveness, metastases development, angiogenesis, and immune suppression.
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