24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now
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Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of human VCAM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
The human VCAM1 ELISA kit is a solid-phase immunoassay specially designed to quantitatively measure human VCAM1 in serum, plasma, cell culture supernates, or tissue homogenates. It is based on the Sandwich-ELISA mechanism. VCAM1 in the sample is bound to the capture antibody immobilized on the 96-well strip plate and then sandwiched with the biotinylated VCAM1 antibody. After the addition of HRP-avidin and TMB substrate, the solution in the wells turns blue. The color reaction is stopped by adding the stop solution into the wells, and the color changes from blue to yellow. The color intensity is positively proportional to the VCAM1 bound in the initial step. The VCAM1 concentration can be calculated according to the standard curve. This kit is tested with high sensitivity, strong specificity, good linearity, high precision and recovery, as well as lot-to-lot consistency.
VCAM1 (CD106) is inducibly and predominantly expressed in endothelial cells. Pro-inflammatory cytokines, such as TNFα, as well as ROS, oxidized low-density lipoprotein, elevated glucose levels, TLR agonists, and shear stress, all promote and increase VCAM1 expression. TNFα increases the expression of VCAM1 on the surface of endothelial cells during inflammation. VCAM1 on active endothelial cells interacts directly with α4β1 integrin on leukocytes, activating VCAM1 downstream signaling molecules such as Ca2+, Rac1, NOX2, ROS, MMPs, PKCα, and PTP1B. These signals eventually cause the affinity of junction adhesion molecules within endothelial cell junctions to relax, allowing leukocytes to pass through. VCAM1 is implicated in the pathogenesis of many immunological disorders, such as rheumatoid Arthritis (RA) and asthma. VCAM1 is also linked to angiogenesis and metastasis in cancer.
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