Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

VCAM1

Uniprot No.

P19320

Research Area

Cell Adhesion

Alternative Names

CD106; CD106 Antigen; INCAM 100; INCAM-100; L1CAM; MGC99561; V-CAM 1; Vascular Cell Adhesion Molecule 1; Vascular cell adhesion protein 1; VCAM 1; VCAM-1; VCAM1; VCAM1_HUMAN

Species

Homo sapiens (Human)

Source

Yeast

Expression Region

25-698aa

Target Protein Sequence

FKIETTPESRYLAQIGDSVSLTCSTTGCESPFFSWRTQIDSPLNGKVTNEGTTSTLTMNPVSFGNEHSYLCTATCESRKLEKGIQVEIYSFPKDPEIHLSGPLEAGKPITVKCSVADVYPFDRLEIDLLKGDHLMKSQEFLEDADRKSLETKSLEVTFTPVIEDIGKVLVCRAKLHIDEMDSVPTVRQAVKELQVYISPKNTVISVNPSTKLQEGGSVTMTCSSEGLPAPEIFWSKKLDNGNLQHLSGNATLTLIAMRMEDSGIYVCEGVNLIGKNRKEVELIVQEKPFTVEISPGPRIAAQIGDSVMLTCSVMGCESPSFSWRTQIDSPLSGKVRSEGTNSTLTLSPVSFENEHSYLCTVTCGHKKLEKGIQVELYSFPRDPEIEMSGGLVNGSSVTVSCKVPSVYPLDRLEIELLKGETILENIEFLEDTDMKSLENKSLEMTFIPTIEDTGKALVCQAKLHIDDMEFEPKQRQSTQTLYVNVAPRDTTVLVSPSSILEEGSSVNMTCLSQGFPAPKILWSRQLPNGELQPLSENATLTLISTKMEDSGVYLCEGINQAGRSRKEVELIIQVTPKDIKLTAFPSESVKEGDTVIISCTCGNVPETWIILKKKAETGDTVLKSIDGAYTIRKAQLKDAGVYECESKNKVGSQLRSLTLDVQGRENNKDYFSPE
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

76.2kDa

Protein Length

Extracellular Domain

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

A truncated version of Vascular cell adhesion protein 1 (VCAM1) encoding the extracellular domain and carrying a 6xHis-tag at the N-terminus was produced in the yeast. The resulting protein is a partial-length VCAM1 protein. It contains aa of 25-698 of the human VCAM1 protein. It was purified by SDS-PAGE and reached up to 90% in purity. On the reducing SDS-PAEG gel, this VCAM1 protein migrated on the molecular weight band of about 66-116 kDa. In addition to producing specific antibodies, the recombinant VCAM1 protein could also be used in the studies of VACM-related immunology or signal transduction.

VCAM-1, or called CD106, is a glycoprotein primarily expressed in endothelial cells. Pro-inflammatory cytokines, ROS, high glucose concentration, oxidized low-density lipoprotein, shear stress, and toll-like receptor agonists, can induce the expression of VCAM-1. VCAM-1 participates in the modulation of inflammation-associated vascular adhesion and the transendothelial migration of leukocytes. Recent studies show the close link of VCAM-1 to the progression of various immunological disorders, such as asthma, rheumatoid arthritis, transplant rejection, and cancer.

Target Background

Function

Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.

Gene References into Functions

High VCAM1 expression is associated with Cardiovascular Disease. PMID: 28496046
not a specific screening marker for pulmonary arterial hypertension in systemic sclerosis PMID: 29687288
We observed an increase of VCAM-1 urine levels in diabetic nephropathy-basal patients compared to diabetic controls and an increase of urinary neprilysin in DN-treated patients with persistent albuminuria PMID: 29854824
Urinary angiostatin and VCAM-1 are predictive of specific histological changes in concurrent lupus nephritis renal biopsies. PMID: 29076253
In summary, it could be concluded that the expression of gene VCAM-1 was down regulated by taurocholic acid through miR-590-3p and that gene VCAM-1 could participate in the regulation of cell growth, cell proliferation and cell apoptosis PMID: 28851649
VCAM-1 expression is upregulated by CD34+/CD133+-stem cells derived from septic patients PMID: 29601599
Circulating soluble vascular cell adhesion molecule (VCAM-1) level was a useful diagnostic parameter for understanding the difference between coronary artery disease (CAD) and cardiac syndrome X (CSX). PMID: 28675064
findings indicate a novel role for JAK2-V617F in activation of beta1 integrins and enhanced adhesion of granulocytes and 32D myeloid progenitors to VCAM1-coated surfaces PMID: 28096537
High VCAM1 expression is associated with Cardiovascular Disease Risk. PMID: 28202840
VCAM-1 levels are elevated in serum of cystic fibrosis patients. PMID: 28646244
VCAM-1 levels are able to accurately predict significant fibrosis in non-alcoholic fatty liver disease patients PMID: 28461687
These results revealed the effect of CD106 and NF-kappaB in bone marrow failure of Acquired aplastic anemia. PMID: 28764810
Overexpression of LINC00341 suppresses the expression of vascular cell adhesion molecule 1 (VCAM1) and the adhesion of monocytes induced by atheroprone flow and tumor necrosis factor-alpha. PMID: 28500253
Increased expression of PECAM-1, ICAM-3, and VCAM-1 in colonic biopsies from patients with inflammatory bowel disease (IBD) in clinical remission is associated with subsequent flares; this suggests that increases in the expression of these proteins may be early events that lead to flares in patients with IBD PMID: 27552332
Association with the efficiency of hematopoietic stem cells (HSC) mobilization to PB were found in the SNPs of the DGKB gene involved in cell transport and SDF-1-induced migration ability and of the VCAM1 gene which is essential for HSC homing, suggesting that SNPs involved in cell migration ability might be partly involved in HSC PMID: 27839966
sVCAM-1 reflects xerostomia in primary Sjogren's syndrome. sICAM-1 and sE-selectin may be additional parameters of secondary Sjogren's syndrome activity. PMID: 29068581
Augmented expression of endothelial adhesion molecules ICAM1/VCAM1 is involved in the pathophysiology of patients with antiphospholipid syndrome. PMID: 29096830
VCAM-1 knockdown reduced TNFalpha-induced HUVEC migration and tube formation. PMID: 28209985
High levels of serum ICAM-1 was associated with the development of multiple organ failure . High levels of VCAM-1 was associated with both multiple organ failure and in-hospital mortality. PMID: 27701021
VCAM1 protein expression increased in cartilage tissue from spinal tuberculosis patients. PMID: 28829887
This study provided direct evidence that increasing extracellular Ca(2+) enhanced TNF-alpha-induced VCAM-1 activation and monocytes adhesion. Moreover, we identified a novel TRPC1/ERK1/2/NFkappaB signaling pathway mediating VCAM-1 activation and monocyte adhesion in this pathological process. PMID: 28583863
Findings suggest that sVCAM-1 is related to affective temperaments, and it is a trait marker for liability to mood disorders. This relationship between alterations in cellular adhesion and affective temperament may be important for vulnerability to affective disorders. PMID: 27693464
patients with newly-diagnosed MM have increased serum levels of VCAM-1 and ICAM-1 that correlate with advanced disease features. VCAM-1 is also independently correlated with survival, suggesting an important role in the biology of the disease and supporting its targeting with novel anti-myeloma drugs. PMID: 27232930
VCAM-1 was statistically elevated in major depressive disorder. PMID: 27598970
Data show that podoplanin (PDPN), CD106 (VCAM-1) and CD248 protein were increased in diseased compared to healthy tendon cells. PMID: 28122639
Peptide nanoparticles targeting VCAM-1 are suggested as a theranostic carrier for drug delivery to inflamed endothelium. PMID: 27105996
IRF-5 targeted the expression of vascular cell adhesion molecule 1 (VCAM-1) at the transcriptional level by binding to its promoter. PMID: 28818665
In patients with optic neuritis that developed into multiple sclerosis, there was a decrease in serum VCAM-1. PMID: 27806869
There was a negative correlation of miR-126 with the PCI-induced markers of inflammation such as hs-CRP and VCAM-1 PMID: 27900989
this study shows that quercetin reduces the expression of VCAM-1 in oxidized LDL cholesterol-induced endothelial cells PMID: 27585526
Gastrin-releasing peptide (GRP) increased the expression of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) by activating nuclear factor-kappaB (NF-kappaB) in endothelial cells. PMID: 28093230
Mesothelium VCAM-1 expression is negatively associated with progression-free and overall survival in EOC. This is especially compelling in light of previous data suggesting that persistent VCAM-1 expression during treatment is an indicator of platinum resistance. PMID: 28263384
The results of this study suggest that VCAM-1 gene variants may be related to obesity and inflammatory markers in the Korean population PMID: 27853845
Panax notoginseng saponins and Ginsenoside protect aorta form atherosclerosis via Rb1 via activation of Nrf2 and suppression of p38 - VCAM-1 signal pathway. PMID: 27620662
Our results suggest that an impairment in adhesion exists in vitiligo skin, which is supported by the diminished immunohistochemical expression of laminin, beta1 integrin, ICAM-1 and VCAM-1. PMID: 28214208
Th1-induced CD106 expression mediates leukocytes adhesion on synovial fibroblasts from juvenile idiopathic arthritis patients via TNF-alpha. PMID: 27123929
Although the expression of VCAM-1 was higher in adult T-cell leukemia/lymphoma patients individuals, there was no significant difference between the studied groups. PMID: 28110427
Activin A and preeclamptic serum upregulate ET-1, ICAM-1, and VCAM-1 in human umbilical vein endothelial cells, consistent with activin A contributing to the pathophysiology of preeclampsia. PMID: 27155341
Statistically significant associations were identified between the chronic periodontal patients and the controls in the gene polymorphisms of rs5498 ICAM-1 (P=0.007) and rs1041163 VCAM-1 (P=0.029). The distribution of rs5498 (P=0.029) and rs1041163 (P=0.049) differed significantly across the mild, moderate, and severe groups of periodontitis. PMID: 27391418
VCAM-1 had a synergistic effect with coronary artery disease on the BDNF PMID: 28131673
findings suggest that the increased levels of IL-17,Hcy and sVCAM contributes contributes to the vascular inflammation and activation presented by sickle cell anemia patients PMID: 26334689
VCAM-1 plays an important role in the development and generation of gestational hypertension. PMID: 27706617
gLDL stimulate the expression of cellular VCAM-1, the secretion of soluble VCAM-1, and the adhesion of monocytes through mechanisms involving p38 MAP kinase and NF-kB signalling pathways activated by RAGE, ERS and oxidative stress, thus contributing to diabetic atherosclerosis. PMID: 27206739
High VCAM1 expression is associated with non-insulin resistant women with polycystic ovary syndrome. PMID: 27245110
Denbinobin inhibited IL-1beta-induced ICAM-1/VCAM-1 expression and monocyte adhesion to osteoarthritis fibroblast-like synoviocytes via mirn146-a PMID: 25052989
Theobroma cacao extract prohibits the increase in IL-6 and sVCAM-1 in endothelial cells induced by plasma from preeclamptic patients. PMID: 26955771
BD patients have a lower expression of ICAM-1, VCAM-1, and E-selectin in their tumor tissues. BD-HDL facilitates the adhesion of tumor cells to vascular endothelium by upregulating the expression of ICAM-1 and VCAM-1, thereby promoting the initial progression of breast cancer metastasis PMID: 26872904
Clopidogrel diminishes TNFalpha-stimulated VCAM-1 expression at least in part via HO-1 induction and CaMKKbeta/AMPK/Nrf2 pathway in endothelial cells. PMID: 26824050
Results show that VCAM1 expression is regulated by TSPO to activate endothelial cells. PMID: 26608360
Human placental, chorionic villi-derived mesenchymal stem cells expressing VCAM-1 promote angiogenesis in a mouse ischemia model. PMID: 27044487

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Subcellular Location

Membrane; Single-pass type I membrane protein.

Tissue Specificity

Expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue.

Database Links

HGNC: 12663

OMIM: 192225

KEGG: hsa:7412

STRING: 9606.ENSP00000294728

UniGene: Hs.109225

Code	CSB-YP025809HU
Abbreviation	Recombinant Human VCAM1 protein, partial
MSDS	MSDS Datasheet
Size	$250
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Vascular cell adhesion protein 1 (VCAM1), partial

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