Human bone morphogenetic protein 4 (BMP4) ELISA Kit

Code CSB-E17298h
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P12644
Lead Time 3-5 working days
Abbreviation BMP4
Protein Biological Process 1 Developmental Protein
Target Name bone morphogenetic protein 4
Alias BMP2B, BMP2B1, MCOPS6, OFC11, ZYME, bone morphogenetic protein 2B
Species Homo sapiens (Human)
Protein Biological Process 3 Chondrogenesis
Sample Types serum, plasma, cell culture supernates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. This particular family member plays an important role in the onset of endochondral bone formation in humans, and a reduction in expression has been associated with a variety of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva. Alternative splicing in the 5 untranslated region of this gene has been described and three variants are described, all encoding an identical protein.
HGNC 1071
RGD 2213
MGI 88180
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human BMP4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %94
Range %89-97
1:2Average %103
Range %95-107
1:4Average %92
Range %82-94
1:8Average %95
Range %89-99
Recovery
The recovery of human BMP4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9490-98
EDTA plasma (n=4)8680-91
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.447 2.542 2.495 2.371
5001.524 1.628 1.576 1.452
2500.948 0.898 0.923 0.799
1250.501 0.512 0.507 0.383
62.50.307 0.314 0.311 0.187
31.20.256 0.245 0.251 0.127
15.60.197 0.191 0.194 0.070
00.123 0.124 0.124
References
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Function Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction (By similarity).
Involvement in disease Microphthalmia, syndromic, 6 (MCOPS6); Non-syndromic orofacial cleft 11 (OFC11)
Subcellular Location Secreted, extracellular space, extracellular matrix
Protein Families TGF-beta family
Tissue Specificity Expressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines.
Database Links

HGNC: 1071

OMIM: 112262

KEGG: hsa:652

STRING: 9606.ENSP00000245451

UniGene: Hs.68879

Pathway Hippo signaling pathway
TGF-beta signaling pathway
Signaling pathways regulating pluripotency of stem cells

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