BMP4 Antibody

Immunohistochemistry of paraffin-embedded human prostate cancer using CSB-PA05799A0Rb at dilution of 1:100

Immunohistochemistry of paraffin-embedded human lung tissue using CSB-PA05799A0Rb at dilution of 1:100

Western Blot
Positive WB detected in: Mouse brain tissue, Mouse skeletal muscle tissue, Zebrafish tissue
All lanes: BMP4 antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa

IHC image of CSB-PA05799A0Rb diluted at 1:600 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA05799A0Rb diluted at 1:600 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.