BMP4 Antibody

Datasheet
Code CSB-PA05799A0Rb
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Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
Uniprot No. P12644
Image
  • Immunohistochemistry of paraffin-embedded human prostate cancer using CSB-PA05799A0Rb at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human lung tissue using CSB-PA05799A0Rb at dilution of 1:100

  • Western Blot
    Positive WB detected in: Mouse brain tissue, Mouse skeletal muscle tissue, Zebrafish tissue
    All lanes: BMP4 antibody at 3µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 47 kDa
    Observed band size: 47 kDa

  • IHC image of CSB-PA05799A0Rb diluted at 1:600 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-PA05799A0Rb diluted at 1:600 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunogen Recombinant Human Bone morphogenetic protein 4 protein (303-408AA)
Raised in Rabbit
Species Reactivity Human, Mouse, Zebrafish
Tested Applications ELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:100-1:1000
Relevance Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Bone morphogenetic protein 4 (BMP-4) (Bone morphogenetic protein 2B) (BMP-2B), BMP4, BMP2B DVR4
Immunogen Species Human
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Research Area Cardiovascular
Target Names BMP4
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
References
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Function Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction (By similarity).
Involvement in disease Microphthalmia, syndromic, 6 (MCOPS6); Non-syndromic orofacial cleft 11 (OFC11)
Subcellular Location Secreted, extracellular space, extracellular matrix
Protein Families TGF-beta family
Tissue Specificity Expressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines.
Database Links

HGNC: 1071

OMIM: 112262

KEGG: hsa:652

STRING: 9606.ENSP00000245451

UniGene: Hs.68879

Pathway Hippo signaling pathway
TGF-beta signaling pathway
Signaling pathways regulating pluripotency of stem cells

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