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| Code | CSB-E09003h |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of human PDI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 95 | ||||
| Range % | 89-99 | |||||
| 1:10 | Average % | 98 | ||||
| Range % | 93-103 | |||||
| 1:20 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:40 | Average % | 95 | ||||
| Range % | 87-99 | |||||
| The recovery of human PDI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 94-101 | ||||
| EDTA plasma (n=4) | 88 | 82-92 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The Human Protein Disulfide Isomerase (PDI) ELISA Kit is an essential tool for quantitative detection of PDI in human serum, plasma, and tissue homogenates. This ELISA kit is specifically designed for research in the field of cancer and cardiovascular, allowing researchers to accurately measure PDI levels with a high level of sensitivity and a wide detection range.
The PDI protein, also known as protein disulfide-isomerase, plays a crucial role in protein folding and disulfide bond formation, making it a key player in various cellular processes. Our PDI ELISA kit is based on a sandwich assay principle, providing reliable and reproducible results with minimal interference from other proteins.
With a sensitivity of 0.078 ng/mL and a detection range of 0.312 ng/mL-20 ng/mL, this ELISA kit can accurately measure PDI levels in a variety of samples. The assay time ranges from 1-5 hours, and the sample volume required is 50-100ul. The detection wavelength is 450 nm, ensuring accurate and precise measurement of PDI levels.
This ELISA kit is an excellent choice for researchers studying cancer and cardiovascular. By providing accurate and sensitive measurements of PDI levels, our ELISA kit can help researchers gain a deeper understanding of the role of PDI in cancer and potentially identify new therapeutic targets.
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