Name: Recombinant Human Protein disulfide-isomerase(P4HB) (Active)
Brand: CUSABIO
SKU: CSB-AP000091HU

Product Details

Purity	>95% as determined by SDS-PAGE and HPLC.
Endotoxin	Less than 1.0 EU/μg as determined by LAL method.
Activity	Thiol Protein Reductase Activity is 0.001 Δ650nm/ min-2, determined by measuring the turbidityincrease at 650 nm due to insulin reduction.The activity is expressed as the ratio of the slope of a linear part of the turbidity curve to the lag time.
Target Names	P4HB
Uniprot No.	P07237
Research Area	Signal Transduction
Alternative Names	Cellular thyroid hormone binding protein; Cellular thyroid hormone-binding protein; Collagen prolyl 4 hydroxylase beta; Disulphide Isomerase; DSI; EC 5.3.4.1; Endoplasmic reticulum resident protein 59; ER protein 59; ERBA2L; ERp59; GIT; Gltathione insulin transhydrogenase; Glutathione insulin transhydrogenase; P4HB; P4Hbeta; p55; PDI; PDIA1; PDIA1_HUMAN; PDIR; PHDB; PO4DB; PO4HB; Procollagen proline 2 oxoglutarate 4 dioxygenase (proline 4 hydroxylase) beta polypeptide (protein disulfide isomerase associated 1); Procollagen proline 2 oxoglutarate 4 dioxygenase beta subunit; PROHB; Prolyl 4 hydroxylase beta polypeptide; Prolyl 4 hydroxylase beta subunit; Prolyl 4 hydroxylase subunit beta; Prolyl 4-hydroxylase subunit beta; Protein disulfide isomerase associated 1; Protein disulfide isomerase, family A, member 1; Protein disulfide isomerase/oxidoreductase; Protein disulfide-isomerase; Protocollagen hydroxylase; Thbp; Thyroid hormone binding protein p55 ; Thyroid hormone binding protein p55 cellular; V erb a avian erythroblastic leukemia viral oncogene homolog 2 like
Species	Homo sapiens (Human)
Source	E.Coli
Expression Region	19-508aa
Complete Sequence	MRGSGSHHHHHH+ APEEEDHVLVLRKSNFAEALAAHKYLLVEFYAPWCGHCKALAPEYAKAAGKLKAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFFRNGDTASPKEYTAGREADDIVNWLKKRTGPAATTLPDGAAAESLVESSEVAVIGFFKDVESDSAKQFLQAAEAIDDIPFGITSNSDVFSKYQLDKDGVVLFKKFDEGRNNFEGEVTKENLLDFIKHNQLPLVIEFTEQTAPKIFGGEIKTHILLFLPKSVSDYDGKLSNFKTAAESFKGKILFIFIDSDHTDNQRILEFFGLKKEECPAVRLITLEEEMTKYKPESEELTAERITEFCHRFLEGKIKPHLMSQELPEDWDKQPVKVLVGKNFEDVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIVIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNGERTLDGFKKFLESGGQDGAGDDDDLEDLEEAEEPDMEEDDDQKAVKDEL
Mol. Weight	56.6 kDa
Protein Length	Partial
Tag Info	N-terminal 6xHis-tagged
Form	Lyophilized powder
Buffer	Lyophilized from a 0.2 µm filtered PBS, pH 7.0
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs	Protein FAQs
Storage Condition	Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time	Basically, we can dispatch the products out in 5-10 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA	Please contact us to get it.

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Target Data

Function	This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration
Gene References into Functions	Peroxynitrite preferentially oxidizes the dithiol redox motifs of protein-disulfide isomerase. PMID: 29191937 Overexpression of HIF-1alpha and P4HB is associated with poor prognosis in patients with gastric cancer. PMID: 29904245 Study demonstrated that the expression of P4HB is frequently upregulated at the mRNA and protein levels in diffuse gliomas. Its high expression was significantly correlated with high Ki-67, more TP53 mutations and poor survival outcome. These findings imply that high expression of P4HB plays an important role in diffuse glioma progression. PMID: 29207176 identify a potent and selective PDIA1 inhibitor, KSC-34, with 30-fold selectivity for the a site over the a' site. KSC-34 displays time-dependent inhibition of PDIA1 reductase activity in vitro with a kinact/ KI of 9.66 x 10(3) M(-1) s(-1) and is selective for PDIA1 over other members of the PDI family, and other cellular cysteine-containing proteins. PMID: 29521097 P4HB promotes hepatocellular carcinoma progression by down-regulating GRP78 expression and subsequently promoting epithelial-to-mesenchymal transition. PMID: 28052026 analysis of antiplatelet activity of CxxC through binding to Cys400 in the PDI a0 domain, which can be further exploited as a model for sitedriven antithrombotic agent development PMID: 28109047 Current findings indicate that thiol isomerase-mediated disulfide bond modification in receptors and plasma proteins is an important layer of control of thrombosis and vascular function more generally. PMID: 28598864 DIA1 was robustly secreted by physiological levels of arterial laminar shear in endothelial cells and supported alpha 5 integrin thiol oxidation. PMID: 28034831 Kinetic-based trapping by intervening sequence variants of the active sites of protein-disulfide isomerase identifies platelet protein substrates. PMID: 28364042 a mechanism of dual Ero1alpha regulation by dynamic redox interactions between PDI and the two Ero1alpha flexible loops that harbor the regulatory cysteines. PMID: 27703014 analysis of how redox affects human protein disulfide isomerase regulate binding affinity of 17 beta-estradiol PMID: 28257787 These findings improve our understanding of PDI-protected aggregation of wild-type alpha-Syn and its H50Q familial mutant. PMID: 27142583 Association of P4HB polymorphisms with sporadic amyotrophic lateral sclerosis susceptibility in the Chinese Han population. PMID: 26000911 the effect of the endoplasmic reticulum chaperone protein disulfide isomerase (PDI) on beta-cell dysfunction, was examined. PMID: 26607804 Amyotrophic lateral sclerosis-linked PDIA1 mutations disrupt motor neuron connectivity. PMID: 26869642 direct binding of PDIA1 to VWF, is reported. PMID: 26670633 Selective sequestration of PDI1A in a calcium depletion-mediated complex with the abundant chaperone calreticulin attenuates the effective concentration of this major lumenal thiol oxidant. PMID: 25575667 Cole-Carpenter syndrome is caused by a specific de novo mutation in P4HB that impairs the disulfide isomerase activity of protein disulfide isomerase. PMID: 25683117 PDI has a role as a competent regulator and a specific substrate of Ero1alpha govern efficient and faithful oxidative protein folding and maintain the ER redox homeostasis PMID: 25258311 The crystal structure of the dimeric form of noncatalytic bb' domains of human PDIA1 determined to 2.3 A resolution revealed that the formation of dimers occludes the substrate binding site. PMID: 24549644 Elevated P4HB expression is associated with temozolomide resistance in malignant glioma. PMID: 23444257 ). The results suggest that P4HB is a modifier gene inamyotrophic lateral sclerosis susceptibility and may represent a potential therapeutic target for amyotrophic lateral sclerosis . PMID: 23337974 A mixed disulfide complex was formed with the catalytic domain A1 from human PDI consistent with a model for cotranslational oxidative protein folding wherein PDI acts as a placeholder that is relieved by the pairing of cysteines caused by substrate folding. PMID: 23141538 TPM4, PDIA and SRC8 were also localized to the trophoblast cells, further highlighting the importance of these cytoskeletal remodelling proteins in early pregnancy PMID: 21373848 these data revealed a redox-regulated chaperone function of PDI in delivering antigenic peptides from TAP to MHC-I. PMID: 21299467 Functional PDI is rapidly secreted from human umbilical vein endothelial cells in culture upon activation with thrombin or after laser-induced stimulation. PMID: 20668226 domain c is required for the stabilization and maintenance of the chaperone function of PDI under extreme conditions PMID: 15358778 data indicate that binding sites in three PDI domains, a, b', and a', contribute to efficient C-P4H tetramer assembly PMID: 15590633 PDI can be S-nitrosated and PDI-SNO can be denitrosated by PDI suggesting that this enzyme could be intimately involved in the transport of intracellular NO equivalents to the cell surface. PMID: 15611098 Data show that protein disulfide isomerase can switch its conformation from dimer to tetramer in its functions as a foldase. PMID: 15695804 Show More Hide All
Involvement in disease	Cole-Carpenter syndrome 1 (CLCRP1)
Subcellular Location	Endoplasmic reticulum, Endoplasmic reticulum lumen, Melanosome, Cell membrane, Peripheral membrane protein
Protein Families	Protein disulfide isomerase family
Database Links	HGNC: 8548 OMIM: 112240 KEGG: hsa:5034 STRING: 9606.ENSP00000327801 UniGene: Hs.464336

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Code	CSB-AP000091HU
Size	US$1448Purchase it in Cusabio online store (only available for customers from the US)
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Recombinant Human Protein disulfide-isomerase(P4HB) (Active)

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