Monkey Interleukin-8,IL-8 ELISA Kit

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Monkey Interleukin-8,IL-8 ELISA Kit

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Protocol

Code	CSB-E08051Mo-IS
Size	96T,5×96T,10×96T
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Product Details
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Customer Reviews and Q&A
Target Background

Target Name

interleukin 8

Alternative Names

CXCL8 ELISA kit; IL8Interleukin-8 ELISA kit; IL-8 ELISA kit; C-X-C motif chemokine 8 ELISA kit; Chemokine ELISA kit; C-X-C motif) ligand 8 ELISA kit

Abbreviation

CXCL8

Uniprot No.

P67813

Species

Monkey

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

7.8pg/mL-500pg/mL

Sensitivity

3.9pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of monkey IL-8/CXCL8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:5	Average %	88
	Range %	84-95
1:10	Average %	92
	Range %	85-99
1:20	Average %	97
	Range %	90-104
1:40	Average %	103
	Range %	99-109

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
500	2.654	2.588	2.621	2.449
250	1.578	1.637	1.608	1.436
125	1.187	1.098	1.143	0.971
62.5	0.683	0.719	0.701	0.529
31.2	0.487	0.471	0.479	0.307
15.6	0.335	0.343	0.339	0.167
7.8	0.295	0.278	0.287	0.115
0	0.174	0.170	0.172

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function
(From Uniprot)

IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus.

Gene References into Functions

Stress decreased IL-8 gene expression in both CeA lesioned and non-lesioned animals. But, CeA lesions increased the tissue expression of IL-8 mRNA prior to and after stress exposure. PMID: 16574374

Subcellular Location

Secreted.

Protein Families

Intercrine alpha (chemokine CxC) family

Database Links

KEGG: mcc:613028

STRING: 9544.ENSMMUP00000005115

UniGene: Mmu.3363

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Related pathways

Chemokine signaling pathway NF-kappa B signaling pathway Cellular senescence IL-17 signaling pathway NOD-like receptor signaling pathway Toll-like receptor signaling pathway AGE-RAGE signaling pathway in diabetic complications Oxidative Phosphorylation Phospholipase D signaling pathway

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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