Mouse Anti-Cyclic Citrullinated Peptide Antibody (Anti-CCP-antibody) ELISA kit

Code CSB-EQ027743MO
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
Anti-Cyclic Citrullinated Peptide Antibody (Anti-CCP-antibody)
Abbreviation
Anti-CCP-Ab
Species
Mus musculus (Mouse)
Sample Types
serum, plasma
Detection Range
6.25 U/mL-400 U/mL
Sensitivity
1.56 U/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse anti-CCP-antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 95  
Range % 88-101  
1:2 Average % 97  
Range % 91-106  
1:4 Average % 95  
Range % 90-106  
1:8 Average % 96  
Range % 89-104  
Recovery
The recovery of mouse anti-CCP-antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 97 90-110  
EDTA plasma (n=4) 95 83-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
U/ml OD1 OD2 Average Corrected  
400 2.697 2.741 2.719 2.573  
200 2.201 2.224 2.213 2.067  
100 1.507 1.483 1.495 1.349  
50 0.834 0.849 0.842 0.696  
25 0.492 0.511 0.502 0.356  
12.5 0.297 0.310 0.304 0.158  
6.25 0.226 0.238 0.232 0.086  
0 0.148 0.144 0.146    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse Anti-CCP-Ab ELISA Kit was designed for the quantitative measurement of Mouse Anti-CCP-Ab protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 6.25 U/mL-400 U/mL and the sensitivity is 1.56 U/mL.

Customer Reviews and Q&A

 Customer Reviews
Average Rating:
5.0 - 2 reviews

Submit a Review here

Sample type: Cell culture supernatant

Sample species: Cells

Review: The kits have nice quality, the tests are stable and the markers are good.

By Anonymous

Sample type: Cell culture supernatant

Sample species: Cells

Review: I used the anti-CCP kit to test serum samples from normal, model and drug administration groups of mice. The data were relatively stable with little intra-group variation. The differences in the retest values of individual samples are also very small, and the quality of the kit is stable. Previously bought the kit remaining a few holes. Probably, after more than six months and then use, it performs very good, praise! I hope CUSABIO continues to maintain!

By Anonymous

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