Mouse CD81 antigen(CD81) ELISA kit

Instructions
Code CSB-EL004960MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name CD81 molecule
Alternative Names Cd81 ELISA Kit; Tapa1CD81 antigen ELISA Kit; 26 kDa cell surface protein TAPA-1 ELISA Kit; Target of the antiproliferative ELISA Kit 1 ELISA Kit; CD antigen CD81 ELISA Kit
Abbreviation CD81
Uniprot No. P35762
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 47 pg/mL-3000 pg/mL
Sensitivity 11.75 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse CD81 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 97
Range % 92-101
1:2 Average % 91
Range % 85-96
1:4 Average % 84
Range % 80-88
1:8 Average % 95
Range % 95-99
Recovery
The recovery of mouse CD81 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 93 89-98
EDTA plasma (n=4) 104 100-113
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
3000 2.692 2.600 2.646 2.497
1500 2.057 1.938 1.998 1.849
750 1.376 1.428 1.402 1.253
375 0.909 0.854 0.882 0.733
187.5 0.589 0.609 0.599 0.450
94 0.365 0.371 0.368 0.219
47 0.239 0.247 0.243 0.094
0 0.152 0.145 0.149  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Background

Function
(From Uniprot)
May play an important role in the regulation of lymphoma cell growth. May be involved in the acrosome reaction.
Gene References into Functions
  1. The species-specific traits in CD9 and CD81 distribution during sperm maturation were compared between mice and humans. A mutual position of CD9/CD81 is shown in human spermatozoa in the acrosomal cap, however in mice, CD9 and CD81 occupy a distinct area. PMID: 29671763
  2. adoptive transfer of wild-type regulatory T cells into CD81-deficient mice was sufficient to promote tumor growth and metastasis; these findings suggested that CD81 modulates adaptive and innate immune responses PMID: 26329536
  3. CD81-Rac interaction exerts an important regulatory role on the innate and adaptive immunity against bacterial infection and suggests a role for CD81 in the development of novel therapeutic targets during infectious diseases. PMID: 25972472
  4. Plasmodium yoelii sporozoite rhoptry discharge occurs only in the presence of CD81, providing the first direct evidence for a role of CD81 during sporozoite productive invasion. PMID: 24798694
  5. In vitro myotubes lacking CD9P-1 or both CD9 and CD81 fuse with a higher frequency than normal myotubes PMID: 23575678
  6. Data suggest that GPC3 down-regulates hepatocyte proliferation by binding to hedgehog (HH) and down-regulating the HH signaling pathway and binding with CD81, thus making it unavailable to bind to Hhex and causing its nuclear translocation. PMID: 23665349
  7. CD81 interacts with the T cell receptor to suppress signaling. PMID: 23226274
  8. CD81 promotes the microvillus formation and/or extension while tetraspanin CD82 inhibits these events. In addition, CD81 enhances the outward bending of the plasma membrane while CD82 inhibits it. PMID: 22079629
  9. Self-renewing hematopoietic stem cells express CD81 during stress-induced proliferation. PMID: 21931533
  10. CD81 is required for the formation of actin membrane protrusions via Rac1 activation in adhesion-dependent immune cell migration. PMID: 21677313
  11. data indicate that CD81 plays a significant role in the final stages of RPE development, controlling cell number and overall developmental pattern PMID: 20882409
  12. CD81 gene is maternally imprinted, with preferential expression from the paternal allele. PMID: 20579434
  13. CD81 plays a negative role in B cell activation in vitro and in vivo. PMID: 19737782
  14. CD81 expression by T cells is critical for their induction of IL-4 synthesis by B cells PMID: 11978781
  15. Infertility of CD9-deficient mouse eggs is reversed by mouse CD81 PMID: 12086470
  16. the role of CD81 in the regulation of astrocyte and microglial number, perhaps by regulating cell proliferation by a contact inhibition-dependent mechanism. PMID: 12357429
  17. Data show that CD81, a putative receptor for hepatitis C virus, is required on hepatocytes for human Plasmodium falciparum and rodent Plasmodium yoelii sporozoite infectivity. PMID: 12483205
  18. CD81 affects expression of its associated partner CD19 beginning at a B-cell postendoplasmic reticulum compartment where CD81 is necessary for normal trafficking or for surface membrane stability of CD19. PMID: 14530327
  19. CD81 strengthens multivalent VLA-4 contacts within subsecond integrin occupancy without altering intrinsic adhesive properties to low density ligand. CD81 facilitates both VLA-4-mediated leukocyte rolling and arrest on VCAM-1 PMID: 14532283
  20. CD81 is required for the association of the B cell antigen receptor and the CD19/CD21 complex with lipid rafts and for enhanced signaling from rafts upon coligation. PMID: 14688345
  21. CD81 signaling events could be mediated by 14-3-3 adapter proteins, and these signals may be dependent on cellular redox PMID: 14966136
  22. CD9 and CD81 play complementary roles in sperm-egg fusion PMID: 16380109
  23. CD81 plays multiple roles in the processing, intracellular trafficking, and membrane functions of CD19. PMID: 16449649
  24. Murine CD81 does not mediate hepatitis C virus pseudoparticle entry when transfected into human HepG2 tumor cells. PMID: 16641285
  25. Disruption of the mouse CD81 gene led to a reduction in the fecundity of female mice, and CD81-/- eggs had impaired ability to fuse with sperm. PMID: 17290409
  26. We conclude that CD81 represents a potential therapeutic target to interfere with leukocyte infiltration and ameliorate inflammatory neurological damage in MS. PMID: 18586096
  27. tetraspanins CD9 and CD81 regulate cell motility and protease production of macrophages and that their dysfunction may underlie the progression of COPD PMID: 18662991
  28. CD81 is indispensable for an authentic in vivo hepatitis C virus infection. PMID: 19030166
  29. CD81 potentially plays a role in macrophage cell line growth regulation. PMID: 19184252
  30. Inactivation of CD81 by homologous recombination can increase astrocyte and microglial cell proliferation and can lead to anomalously large brain size (30% larger than normal). The effect depends on genetic background. PMID: 12357429

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Subcellular Location Basolateral cell membrane, Multi-pass membrane protein
Protein Families Tetraspanin (TM4SF) family
Tissue Specificity Expressed in oocytes. Highly expressed in granulosa cells.
Database Links

KEGG: mmu:12520

STRING: 10090.ENSMUSP00000043768

UniGene: Mm.806

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