Product Details

Target Name

glypican 3

Alternative Names

Gpc3 ELISA Kit; Glypican-3 [Cleaved into: Glypican-3 alpha subunit; Glypican-3 beta subunit] ELISA Kit

Abbreviation

GPC3

Uniprot No.

Q8CFZ4

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, cell culture supernates

Detection Range

3.12 ng/mL-200 ng/mL

Sensitivity

0.78 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse GPC-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	84-95
1:2	Average %	94
	Range %	84-98
1:4	Average %	94
	Range %	85-97
1:8	Average %	90
	Range %	84-94

Recovery

The recovery of mouse GPC-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	91-103
EDTA plasma (n=4)	95	90-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
200	2.173	2.202	2.188	2.050
100	1.648	1.683	1.666	1.528
50	0.936	0.911	0.924	0.786
25	0.549	0.540	0.545	0.407
12.5	0.301	0.323	0.312	0.174
6.25	0.245	0.251	0.248	0.110
3.12	0.178	0.181	0.180	0.042
0	0.137	0.139	0.138

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Mouse GPC3 ELISA Kit was designed for the quantitative measurement of Mouse GPC3 protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 3.12 ng/mL-200 ng/mL and the sensitivity is 0.78 ng/mL.

Target Background

Function
(From Uniprot)

Cell surface proteoglycan that bears heparan sulfate. Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins. Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation. Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands. Positively regulates the non-canonical Wnt signaling pathway. Binds to CD81 which decreases the availability of free CD81 for binding to the transcriptional repressor HHEX, resulting in nuclear translocation of HHEX and transcriptional repression. Inhibits the dipeptidyl peptidase activity of DPP4. Plays a role in limb patterning and skeletal development by controlling the cellular response to BMP4. Modulates the effects of growth factors BMP2, BMP7 and FGF7 on renal branching morphogenesis. Required for coronary vascular development. Plays a role in regulating cell movements during gastrulation.

Gene References into Functions

Coupling of pGPC3 to liposomes was essential for effective priming of GPC3-specific CTLs. PMID: 26616051
The expression of GPC-3 was altered by DEN treatment. PMID: 24377529
Finding represents a rare four layer genomic overlap consisting of growth associated quantitative trait locus (QTL), body mass associated Gpc3 gene, highly conserved miRNA gene and mature miRNA seed SNP identified in the lean mouse. PMID: 21152117
Hepatocyte overexpression of GPC3 suppresses hepatocyte proliferation and liver regeneration and alters gene expression profiles. PMID: 20812357
glypican-3 is involved in the recruitment of M2-polarized tumor-associated macrophages in hepatocellular carcinoma PMID: 19838081
GPC3 inhibits the PI3K/Akt anti-apoptotic pathway while it stimulates the p38MAPK stress-activated one in murine mammary adenocarcinoma LM3 cells PMID: 19288189
Gpc3 function in development of Simpson-Golabi-Behmel syndrome is IGF-independent PMID: 11846487
GPC3 knockout mice exhibit alterations in the Wnt signaling pathway, which is also associated with the regulation of cell proliferation PMID: 15537637
Polymorphisms in the 3' untranslated region of Gpc3 are strong candidates for the causal sequence variation and loss-of-function mutations can cause overgrowth and developmental abnormalities. PMID: 15799711
Altered hematopoiesis in glypican-3-deficient mice results in decreased osteoclast differentiation and a delay in endochondral ossification. PMID: 15936336
Zhx2 acts as a repressor of Gpc3 in the adult liver. Gpc3 is activated in the regenerating liver in an Afr2-dependent manner. PMID: 17668883
A novel glycosyl phosphatidyl inositol (GPI)-linked proteoglycan, Glypican3 (GPC3, binding to GLUT4 was identified. PMID: 18343214
Glypican-3 regulates migration, adhesion and actin cytoskeleton organization in mammary tumor cells through Wnt signaling modulation. PMID: 18404367
GPC3 acts as a negative regulator of Hedgehog signaling during mammalian development. PMID: 18477453
The heparan sulfate proteoglycan (HSPG) glypican-3 mediates commitment of MC3T3-E1 cells toward osteogenesis. PMID: 19479939
The Gpc3-null mice show increased levels of Ihh protein and signalling, but similar levels of Ihh messenger RNA. PMID: 19590577
Data show that glypican-3-deficient mice display a high incidence of congenital cardiac malformations like ventricular septal defects, common atrioventricular canal and double outlet right ventricle. PMID: 19733558

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Subcellular Location

Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side.

Protein Families

Glypican family

Tissue Specificity

In the developing limb, absent from the apical epidermal ridge at 11 dpc but highly expressed in the underlying mesenchyme. Expression in the mesenchyme at this stage is asymmetric with highest levels in the regions of the distal mesenchyme within the pro

Database Links

KEGG: mmu:14734

STRING: 10090.ENSMUSP00000064131

UniGene: Mm.22515

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Code	CSB-E15877m
Size	96T,5×96T,10×96T
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Mouse Glypican-3(GPC-3)ELISA Kit

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