GPC3

GPC3 is a glycoprotein with a specific protein structure, including an extracellular glycosylated portion and an intracellular portion. It can interact with various growth factors, signaling molecules, and receptors, thereby influencing cell signaling pathways. It can either promote or inhibit the activation of specific signaling pathways, thus playing a regulatory role in processes such as cell growth, proliferation, and differentiation. During embryonic development, GPC3 is crucial for the formation of normal organs and tissues. It is involved in establishing the embryonic body axis, limb development, and organ differentiation at specific stages of development. Aberrant expression of GPC3 can impact the onset and progression of diseases, and studying the function and regulatory mechanisms of GPC3 is of significant importance for understanding disease mechanisms and developing relevant therapeutic approaches.

More Interesting read related GPC3: The Next-Promising Target in Cancer Immunotherapy

Validated Data

SDS

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

ELISA

Activity: Measured by its binding ability in a functional ELISA. Immobilized human GPC3 (G537R) at 5 μg/ml can bind Anti-GPC3 recombinant antibody, the EC₅₀ is 4.739-7.092 ng/ml.

● GPC3 Recombinant Monoclonal Antibody CSB-RA009705A0HU

Validated Data

Western Blot
Positive WB detected in: 293 whole cell lysate, K562 whole cell lysate
All lanes: GPC3 antibody at 0.9μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 66, 60, 69 KDa
Observed band size: 66 KDa

Immunofluorescence staining of HepG2 cells with CSB-RA009705A0HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

● CT26/Human GPC3 Stable Cell Line CSB-SC009705HU2

Validated Data

Untransfected CT26 cells (green line) and transfected Human GPC3 CT26 Stable cells (red line) were stained with anti-GPC3 antibody (CSB-RA009705A1HU) (2µg/1*106cells), washed and then followed by APC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.

The following GPC3 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.

GPC3 Antibodies

GPC3 Proteins

GPC3 ELISA Kits

GPC3 Antibodies

GPC3 Antibodies for Homo sapiens (Human)

Code	Product Name	Species Reactivity	Application
CSB-PA009705GA01HU	GPC3 Antibody	Human,Mouse,Rat	ELISA,WB
CSB-PA005135	GPC3 Antibody	Human,Mouse,Rat	WB, ELISA
CSB-RA009705A0HU	GPC3 Recombinant Monoclonal Antibody	Human	ELISA, WB, IF
CSB-PA12749A0Rb	GPC3 Antibody	Human	ELISA, IF
CSB-PA12749B0Rb	GPC3 Antibody, HRP conjugated	Human	ELISA
CSB-PA12749C0Rb	GPC3 Antibody, FITC conjugated	Human
CSB-PA12749D0Rb	GPC3 Antibody, Biotin conjugated	Human	ELISA
CSB-MA009705A0m	GPC3 Monoclonal Antibody	Human	ELISA, WB, FC
CSB-RA009705A1HU	GPC3 Recombinant Monoclonal Antibody	Human	ELISA

GPC3 Proteins

GPC3 Proteins for Rattus norvegicus (Rat)

Code	Product Name	Source
CSB-YP009705RA	Recombinant Rat Glypican-3 (Gpc3), partial	Yeast
CSB-EP009705RA CSB-BP009705RA CSB-MP009705RA CSB-EP009705RA-B	Recombinant Rat Glypican-3 (Gpc3), partial	E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

GPC3 Proteins for Homo sapiens (Human)

Code	Product Name	Source
CSB-YP009705HU CSB-EP009705HU CSB-BP009705HU CSB-EP009705HU-B	Recombinant Human Glypican-3 (GPC3), partial	Yeast E.coli Baculovirus In Vivo Biotinylation in E.coli
CSB-AP005371HU	Recombinant Human Glypican-3 (GPC3), partial (Active)	Mammalian cell
CSB-MP009705HU(M)	Recombinant Human Glypican-3 (GPC3) (G537R), partial (Active)	Mammalian cell

GPC3 Proteins for Pan troglodytes (Chimpanzee)

Code	Product Name	Source
CSB-YP009705EQV CSB-EP009705EQV CSB-BP009705EQV CSB-EP009705EQV-B	Recombinant Pan troglodytes Glypican-3 (GPC3), partial	Yeast E.coli Baculovirus In Vivo Biotinylation in E.coli
CSB-MP009705EQV	Recombinant Pan troglodytes Glypican-3 (GPC3)	Mammalian cell

GPC3 Proteins for Mus musculus (Mouse)

Code	Product Name	Source
CSB-YP804039MO CSB-EP804039MO CSB-BP804039MO CSB-MP804039MO CSB-EP804039MO-B	Recombinant Mouse Glypican-3 (Gpc3), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli